The impact of blastomere survival rates on developmental competence of cryo-thawed Day 2 embryos

O'Shea, Lynne; Hughes, Caroline; Kirkham, Colin; Mocanu, Edgar

doi:10.1016/j.ejogrb.2015.11.040

Cited by 6 publications

(7 citation statements)

References 20 publications

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“…The present study showed that the D3 embryos with blastomere loss had an obvious decreased developmental potential than intact embryos. This finding is mostly in agreement with a series of previous studies [ 13 – 19 ]. The reason for the lower viability of embryos with blastomere loss remains not clearly clarified to date.…”

Section: Discussionsupporting

confidence: 94%

“…Despite so many supportive studies, this issue is still full of controversy. Some studies declared that blastomere loss was not associated with an elevated miscarriage rate [ 17 , 19 ]. Whereas in our study, the biochemical pregnancy rate, one of the embryonic factors putatively involved in the process of implantation [ 29 ], seemed to be higher in the blastomere loss groups (though without statistical significance).…”

Section: Discussionmentioning

confidence: 99%

“…However, other studies have revealed a deleterious effect of blastomere loss on the developmental capacity of embryos, including the in vitro process of mitosis or blastocyst formation and the post-transfer ability of conception [ 13 – 16 ]. Of note, there are also several studies that reported negative influences of blastomere loss in terms of some pregnancy-related indicators, while claiming blastomere loss as harmless regarding the other indices [ 17 – 19 ].…”

Section: Introductionmentioning

confidence: 99%

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The impact of blastomere loss on pregnancy and neonatal outcomes of vitrified-warmed Day3 embryos in single embryo transfer cycles

et al. 2022

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Background Blastomere loss is a common phenomenon that occurs following cryopreservation. To date, studies have drawn conflicting conclusions regarding the impact of blastomere loss on pregnancy outcomes. Besides, limited information is available concerning the neonatal safety of embryos with blastomere loss. In the present study, we aimed to investigate the impact of blastomere loss on pregnancy and neonatal outcomes of vitrified/warmed Day3 cleavage-stage embryos in single embryo transfer cycles. Methods This retrospective cohort study included all vitrified/warmed D3 cleavage-stage single frozen-thawed embryo transfer (FET) cycles between April 2015 and February 2021. We compared pregnancy and subsequent neonatal outcomes between the intact embryos group and the blastomere loss group in single FET cycles. Results A total of 6287 single FET cycles were included in the study, in which 5873 cycles were classified into the intact embryo group and 414 cycles were classified into the blastomere loss group. The outcomes of the blastomere loss group were significantly inferior to those of the intact embryo group, in terms of implantation/biochemical pregnancy/clinical pregnancy/ongoing pregnancy rate and live birth rate per embryo transfer cycle/per clinical pregnancy. Further binary logistic regression confirmed that blastomere loss was negatively associated with live birth. Moreover, the blastomere loss group presented with an elevated early miscarriage rate. The neonatal conditions were broadly similar between the two groups. Additionally, multiple binary logistic regression analysis demonstrated that primary infertility and intracytoplasmic sperm injection (ICSI) were common influencing factors of blastomere loss (aOR 1.447, 95% CI 1.038–2.019, P = 0.029; aOR: 1.388, 95% CI: 1.044–51.846, P = 0.024). Conclusions The transfer of vitrified/warmed D3 embryos with blastomere loss is related to impaired embryo developmental potentials and reduced probabilities of conception. Moreover, even if the embryos with blastomere loss have implanted and reached clinical pregnancies, they present with a lower possibility of developing to live birth owing to a higher early miscarriage rate. However, once the embryos with blastomere loss result in a live birth, no adverse neonatal outcomes are observed. Primary infertility and ICSI were found to be risk factors for blastomere loss.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The impact of blastomere loss on pregnancy and neonatal outcomes of vitrified-warmed Day3 embryos in single embryo transfer cycles

et al. 2022

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“…The modification of the protocol for the new formulation excludes one step which makes the procedure more time-efficient. The impact of blastomere loss on success rates after slow freezing have been well established [15]. However, with loss of a single blastomere, viability is not significantly affected.…”

Section: Discussionmentioning

confidence: 99%

Clinical evaluation of two formulations of slow-freezing solutions for cleavage stage embryos

Jin

et al. 2016

J Assist Reprod Genet

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Purpose The aim was to investigate if improved survival rates could be achieved using a new formulation of solutions for slow freezing of human cleavage stage embryos. Methods The evaluation was divided into two parts. The first part was a retrospective analysis of results obtained after freezing and thawing of day 3 embryos from 400 women using an old formulation of cryopreservation solutions compared to results from 108 women for which cryopreservation had been performed using new compositions of solutions. The second part was prospective, adding cycles until similar numbers of patients had been included in both groups. In total, 2274 embryos from 897 patients were thawed using the old formulation of solutions while 1273 embryos from 542 patients were frozen and thawed using the new solutions. The primary endpoint was survival rate. Results With the new solutions, the survival rate increased from 82.1 to 94.4 % and the complete embryo survival rate increased from 54.9 to 81.3 %. The implantation rate, clinical pregnancy rate per embryo transfer, and per cycle were 28.2, 45.2, and 43.7 %, respectively, using the old formulations of cryosolutions. With the new solutions, the results reached 33.7, 54.1, and 54.1 %, respectively. All differences in results were statistically significant. The number of cancelled embryo transfers due to no survived embryos was 18 with the old solutions and 0 using the new solutions.Conclusion With the new composition of solutions for slow freezing and thawing of embryos, significantly improved results were obtained. Additionally, the number of cancelled embryo transfers was reduced.

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“…Findings are conflicting regarding perinatal outcomes following transfer cycles of cleavage-stage embryos with blastomere loss. Several studies have revealed that the transfer of embryos with blastomere loss results in implantation rates that are comparable to those following the transfer of embryos with intact blastomeres [ 6 – 8 ]. However, other studies have reported that blastomere loss might be associated with a reduction in the implantation rate, impaired in vitro development after thawing and impaired embryo survival following embryo transfer [ 9 – 13 ].…”

Section: Introductionmentioning

confidence: 99%

Outcomes of neonates born following transfers of frozen-thawed cleavage-stage embryos with blastomere loss: a prospective, multicenter, cohort study

Zhu

et al. 2018

BMC Med

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BackgroundDespite limited information on neonatal safety, the transfer of frozen-thawed cleavage-stage embryos with blastomere loss is common in women undergoing in vitro fertilization. We aimed to evaluate the pregnancy outcomes and safety of frozen-thawed cleavage-stage embryos with blastomere loss.MethodsThis prospective, multicenter, cohort study included all frozen-thawed cleavage-stage embryo transfer (FET) cycles between 2002 and 2012. Pregnancy outcomes and subsequent neonatal outcomes were compared between FET cycles with intact embryos and those with blastomere loss.ResultsA total of 12,105 FET cycles were included in the analysis (2259 cycles in the blastomere loss group and 9846 cycles in the intact embryo group). The blastomere loss group showed significantly poorer outcomes with respect to implantation, pregnancy, and live birth rates than the intact embryo group. However, following embryo implantation, the two groups were similar with respect to live birth rates per clinical pregnancy. Among multiple pregnancies (4229 neonates), neonates from the blastomere loss group were at an increased risk of being small for gestational age (aOR = 1.50, 95% CI 1.00–2.25) compared to those from the intact group. A similar trend was observed among singletons (aOR = 1.84, 95% CI 0.99–3.37). No associations were found between blastomere loss and the subsequent occurrence of congenital anomalies or neonatal mortality. However, neonates from the blastomere loss group were at an increased risk of transient tachypnea of the newborn (aOR = 5.21, 95% CI 2.42–11.22).ConclusionsThe transfer of embryos with blastomere loss is associated with reduced conception rates. Once the damaged embryos have implanted, pregnancies appear to have the same probability of progressing to live birth but with an increased risk of small for gestational age neonates and transient tachypnea of the newborn.Study registrationThis study was retrospectively registered at Chinese Clinical Trial Registry. Registration number: ChiCTR-OOC-16007753. Registration date: 13 January 2016.Electronic supplementary materialThe online version of this article (10.1186/s12916-018-1077-8) contains supplementary material, which is available to authorized users.

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The impact of blastomere survival rates on developmental competence of cryo-thawed Day 2 embryos

Cited by 6 publications

References 20 publications

The impact of blastomere loss on pregnancy and neonatal outcomes of vitrified-warmed Day3 embryos in single embryo transfer cycles

The impact of blastomere loss on pregnancy and neonatal outcomes of vitrified-warmed Day3 embryos in single embryo transfer cycles

Clinical evaluation of two formulations of slow-freezing solutions for cleavage stage embryos

Outcomes of neonates born following transfers of frozen-thawed cleavage-stage embryos with blastomere loss: a prospective, multicenter, cohort study

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