The Impact of Black Seeds and Sidr Honey on paracetamol Induced Nephropathy in Adult Male Albino Rats, a Histological, Immunohistochemical & Ultrastructural study

Abstract

Aim of work:The kidneys play an important role in body homeostasis by conserving fluids and electrolytes and by disposing metabolic wastes. Kidney diseases are considered a worldwide public health issue. They contribute to approximately 850,000 deaths each year. Honey is a natural product which is widely used for its therapeutic effects. Also, black seeds have been used in traditional medicine for centuries as it is effective against many diseases. Aim of work:To study the possible protective effect of Sidr honey and black seeds on experimentally induced acute nephropathy in adult male albino rat. Material & Methods:In the present study, twenty -four adult male albino rats were used. They were divided into four groups, 6 rats each. (GI): Control group, (GII) Paracetamol injected group by single intraperitoneal high dose of paracetamol then sacrificed, (GIII): treated by Nigella Sativa and Sidr Honey at the same time for 28 days followed by single intraperitoneal high dose of paracetamol (protective group), (GIV): injected by single intraperitoneal high dose of paracetamol then treated by Nigella Sativa and Sidr Honey at the same time for 28 days (curative group). Blood Samples were collected, serum creatinine, albumin and blood urea nitrogen were estimated. The kidney dissected out and divided into two parts; a part processed and examined by light microscope, and the other processed and examined by electron microscope. Results: Application of paracetamol caused destructive changes in the renal corpuscles and tubules, these changes were ameliorated by application of Sidr honey and black seeds in both protective and curative groups. Conclusion: Sidr honey and black seeds are effective agents in protection and treatment of kidney insults.