The impact of adsorbed cellulase inactivation on enzymatic hydrolysis kinetics.

Ye, Zhuoliang

doi:10.18297/etd/1623

Cited by 2 publications

(1 citation statement)

References 90 publications

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“…In the present work, we optimized the culture conditions to obtain a CBH-enriched supernatant given that specific activities of CBHs are lower than other enzyme components which hydrolyse the crystalline cellulose (Ye, 2012). Unlike the ascomycetes CBHs, the CBHs from basidiomycetes have been poorly explored for cellulose saccharification.…”

Section: Discussionmentioning

confidence: 99%

Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion

Coniglio

Fonseca

Díaz

et al. 2019

Arab Journal of Basic and Applied Sciences

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The production of bioethanol from lignocellulosic biomass comprises the enzymatic hydrolysis of lignocellulosic structures by three major cellulases. Among them, cellobiohydrolases are considered to be key enzymes playing a significant role on cellulose degradation. The ability to produce lignocellulolytic enzymes by fungi such as Trametes villosa makes them appropriate degraders for large-scale applications. In this context, the aim of this study was to obtain and characterize a cellobiohydrolase-enriched extracellular extract of T. villosa LBM 033 (Misiones, Argentina), which is suitable for the enzymatic hydrolysis of lignocellulosic residues. The effect of carbon and nitrogen sources on cellobiohydrolase activity was evaluated using experimental designs and a culture medium was optimized to obtain a cellobiohydrolase-enriched extract suitable for the hydrolysis of lignocellulosic biomass. Moreover, by secretome analysis, nine enzymes involved in lignocellulosic biomass degradation were identified under the optimized conditions; among them is a cellobiohydrolase II from the glycosil-hydrolase 6 family. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion

Coniglio

Fonseca

Díaz

et al. 2019

Arab Journal of Basic and Applied Sciences

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Strategic adsorption/desorption of cellulases NS 50013 onto/from AVICEL PH 101 and protobind 1000

Baig¹

2021

Preprint

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Desorption of active cellulases from lignocellulosic substrates is a potential technique to reuse cellulases for the production of bioethanol. For desorption studies, adsorption of cellulases had to be performed first. Adsorption of cellulases NS 50013 onto microcrystalline cellulose (Avicel PH 101) and wheat straw lignin (Protobind 1000) was studied. It was found that Protobind adsorbed twice the amount of cellulases than Avicel did. An adsorption strategy developed was to work at pH 5 and a temperature less than 323 K to get maximum adsorption on the cellulose component, less adsorption on the lignin component of lignocellulosic materials, and to harmonize adsorption temperature with the industrial hydrolysis situation. Desorption of cellulases from Avicel and Protobind over a range of 298 K to 343 K and a pH of 6 to 9 was studied. Desorption obtained at pH 9 and 333K was optimum for both Avicel and Protobind. Hence, desorption was enhanced by 21 % and 11% for Avicel and Protobind respectively. The cellulases activity for Avicel was 48 FPU mL-1 at pH 9, 333 K, 5% glycerol, representing 91 % of the initial activity and for Protobind, the activity was 33 FPU mL-1 which represents about 66 % of the initial activity. All of these values were higher than ever reported in literature. At pH 5 and 298 K the amount of cellulases desorbed from untreated wheat straw (WS) was 33 % of those initially used for the adsorption step. It was increased to 42 % when 30 % delignified WS was used, and was further increased to 48 % for 60 % delignified WS. Desorption obtained for 60 % delignified WS was 75 % at pH 9, 333K and 5% glycerol. The desorption strategy recommended for bioethanol producing industries, is: 1) removal of lignin; 2) adsorption of cellulases at pH 5 and lower than 323K; 3) hydrolysis of lignocellulosic material; and 4) desorption of cellulases from non-hydrolyzed material at 333 K, pH 9, with 5-10 % glycerol. The proposed strategic desorption of cellulases may reduce the cost of Canadian bioethanol production by 26.5 % due to 75 % recyclability of active cellulases.

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The impact of adsorbed cellulase inactivation on enzymatic hydrolysis kinetics.

Cited by 2 publications

References 90 publications

Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion

Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion

Strategic adsorption/desorption of cellulases NS 50013 onto/from AVICEL PH 101 and protobind 1000

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