The immunomodulatory effects of tachykinins and their receptors

Khorasani, Sahar; Boroumand, Nadia; Arab, Fahimeh Lavi; Hashemy, Seyed Isaac

doi:10.1002/jcb.29668

Cited by 16 publications

(8 citation statements)

References 76 publications

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“…Several studies have recently demonstrated that SP induces a series of signaling pathways through NK1R that mediate cellular excitability in several human tumor cells. It has important roles in forming and spreading various tumor cells via migration, angiogenesis, and metastasis [11][12][13][14][15][16]. is is important since the prevention of metastasis is a major goal in treating tumors.…”

Section: Introductionmentioning

confidence: 99%

The Effect of SP/NK1R on the Expression and Activity of Catalase and Superoxide Dismutase in Glioblastoma Cancer Cells

Korfi

Javid

Darban

et al. 2021

Biochemistry Research International

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Introduction. Glioblastoma is the most malignant brain tumor with different therapeutic protocols, including surgery, radiotherapy, and chemotherapy. Substance P (SP), a peptide released by sensory nerves, increases cellular excitability by activating the neurokinin-1 receptor (NK1R) in several human tumor cells. Aprepitant is a potent and long-lasting NK1R antagonist, considered a new agent for inhibiting proliferation and induction of apoptosis in malignant cells. This study aimed to evaluate the effects of the SP/NK1R system on the expression and activity of catalase and superoxide dismutase (SOD) in the glioblastoma U87 cancer cell line. Methods. Cytotoxicity was measured by the resazurin test, 24 hours after treatment, with increasing aprepitant concentrations. The production of reactive oxygen species (ROS) was also measured 24 hours after treatment with SP and aprepitant. Enzymes activity of catalase and SOD was measured using the corresponding assay kits. Real-time PCR also measured their expression. Results. Aprepitant significantly reduced the viability of U87 cells in a concentration-dependent manner. ROS production was significantly reduced, and the activity of catalase and SOD increased after treatment with aprepitant. The expression of catalase and SOD enzymes also increased significantly in the presence of aprepitant. Conclusion. The present study showed that aprepitant inhibited SP’s oxidizing effects via inducing the antioxidant effects of catalase and SOD in the U87 cell line. Therefore, this drug might be introduced as a potential candidate for controlling glioblastoma cancer in animal models and clinical trials.

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Section: Introductionmentioning

confidence: 99%

The Effect of SP/NK1R on the Expression and Activity of Catalase and Superoxide Dismutase in Glioblastoma Cancer Cells

Korfi

Javid

Darban

et al. 2021

Biochemistry Research International

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“…In mammals, TAC4 gene products were various in different species. TAC4 in mice and rats can encode one hemokinin mature peptide with 11 amino acid length [ 25 ]. In human, TAC4 encodes for hemokinin-1 (hHK-1), but its sequence is different from its murine counterpart.…”

Section: Discussionmentioning

confidence: 99%

Novel Pituitary Actions of TAC4 Gene Products in Teleost

Shi

Cheng

Qin

et al. 2021

IJMS

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Tachykinin 4 (TAC4) is the latest member of the tachykinin family involved in several physiological functions in mammals. However, little information is available about TAC4 in teleost. In the present study, we firstly isolated TAC4 and six neurokinin receptors (NKRs) from grass carp brain and pituitary. Sequence analysis showed that grass carp TAC4 could encode two mature peptides (namely hemokinin 1 (HK1) and hemokinin 2 (HK2)), in which HK2 retained the typical FXGLM motif in C-terminal of tachyinin, while HK1 contained a mutant VFGLM motif. The ligand-receptor selectivity showed that HK2 could activate all 6 NKRs but with the highest activity for the neurokinin receptor 2 (NK2R). Interestingly, HK1 displayed a very weak activation for each NKR isoform. In grass carp pituitary cells, HK2 could induce prolactin (PRL), somatolactin α (SLα), urotensin 1 (UTS1), neuromedin-B 1 (NMB1), cocaine- and amphetamine-regulated transcript 2 (CART2) mRNA expression mediated by NK2R and neurokinin receptor 3 (NK3R) via activation cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA), phospholipase C (PLC)/inositol 1,4,5-triphosphate (IP3)/protein kinase C (PKC) and calcium2+ (Ca2+)/calmodulin (CaM)/calmodulin kinase-II (CaMK II) cascades. However, the corresponding stimulatory effects triggered by HK1 were found to be notably weaker. Furthermore, based on the structural base for HK1, our data suggested that a phenylalanine (F) to valine (V) substitution in the signature motif of HK1 might have contributed to its weak agonistic actions on NKRs and pituitary genes regulation.

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“…Recent studies showed that tachykinins and their receptors regulate the development, activation, and function of critical immune cells and the immune system. Tachykinins are also involved in immune cell migration, immune cell proliferation, and inflammation regulation with cytokines [ 26 , 27 ]. We analyzed the correlation between expression levels of TACR2 and the content of immune cells and found that expression levels of TACR2 positively correlated with mast cells and negatively correlated with M2 macrophages.…”

Section: Discussionmentioning

confidence: 99%

TACR2 is associated with the immune microenvironment and inhibits migration and proliferation via the Wnt/β-catenin signaling pathway in prostate cancer

Wang

2021

Cancer Cell Int

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Background The tachykinin receptor 2 (TACR2) is encoded by the tachykinin receptor correlation gene. Recent microarray analysis for prostate cancer suggests that TACR2 expression is associated with clinical phenotype and disease-free survival among patients with prostate cancer. Results TACR2 protein levels were lower in prostate cancer tissues than in adjacent normal prostate tissue. TACR2 expression significantly correlated with clinical stage, Gleason scores, and survival outcomes. TACR2 expression positively correlated with mast cells and negatively correlated with M2 macrophages. Overexpression of TACR2 promoted the migration and proliferation of prostate cancer cells by regulating the Wnt signaling pathway. Conclusions The TACR2-Wnt/β-catenin signaling pathway is critical in prostate cancer. TACR2 may affect tumor cells’ occurrence and development by changing the content of immune cells in the tumor microenvironment. These findings suggest that TACR2 may be a candidate molecular biomarker for prostate cancer therapy.

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The immunomodulatory effects of tachykinins and their receptors

Cited by 16 publications

References 76 publications

The Effect of SP/NK1R on the Expression and Activity of Catalase and Superoxide Dismutase in Glioblastoma Cancer Cells

The Effect of SP/NK1R on the Expression and Activity of Catalase and Superoxide Dismutase in Glioblastoma Cancer Cells

Novel Pituitary Actions of TAC4 Gene Products in Teleost

TACR2 is associated with the immune microenvironment and inhibits migration and proliferation via the Wnt/β-catenin signaling pathway in prostate cancer

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