The immunological response of pigs and Guinea pigs to antigens of African swine fever virus

Forman, A. J.; Wardley, R.C.; Wilkinson, Philip

doi:10.1007/bf01314703

Cited by 50 publications

(27 citation statements)

References 9 publications

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“…Attempts to vaccinate animals using infected cell extracts, supernatants of infected pig peripheral blood leukocytes, purified and inactivated virions, infected glutaraldehyde-fixed macrophages, or detergenttreated infected alveolar macrophages failed to induce protective immunity (Coggins 1974;Forman et al 1982;Kihm et al 1987;Mebus 1988). Homologous protective immunity does develop in pigs surviving viral infection.…”

Section: Asfv-host Interactionsmentioning

confidence: 95%

African Swine Fever Virus

Tulman

Delhon

et al. 2009

Current Topics in Microbiology and Immunology

132

117

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African swine fever virus (ASFV) is a large, intracytoplasmicallyreplicating DNA arbovirus and the sole member of the family Asfarviridae . It is the etiologic agent of a highly lethal hemorrhagic disease of domestic swine and therefore extensively studied to elucidate the structures, genes, and mechanisms affecting viral replication in the host, virus-host interactions, and viral virulence. Increasingly apparent is the complexity with which ASFV replicates and interacts with the host cell during infection. ASFV encodes novel genes

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Section: Asfv-host Interactionsmentioning

confidence: 95%

African Swine Fever Virus

Tulman

Delhon

et al. 2009

Current Topics in Microbiology and Immunology

132

117

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“…Although traditional neutralizing antibodies against ASFV have not been demonstrated (Costa, 1990;Forman et al, 1982;Vifiuela, 1987), it is evident that antibodies and cytotoxic T lymphocytes are involved in the immune protection of pigs from homologous ASFV challenge or reinfection (Onisk et al, 1994;Martins et al, 1993). The jI3L gene product was immunogenic as recovered pig sera contained antibodies to the predicted antigenic peptide epitope.…”

Section: Immune Potentials Of Jl3l Gene Productmentioning

confidence: 99%

“…The most striking aspect of the immune response against ASFV is the lack of production of traditional virus-neutralizing antibodies, both by pigs recovered from natural infection and by laboratory animals inoculated with virus preparations (Costa, 1990;Forman et al, 1982). However, some immune protection does occur since pigs that have recovered from infection are resistant to homologous virus challenge.…”

Section: Introductionmentioning

confidence: 99%

African swine fever virus gene j13L encodes a 25-27 kDa virion protein with variable numbers of amino acid repeats

Sun

Jacobs

Smith

et al. 1995

Journal of General Virology

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The African swine fever virus (ASFV)j 13L gene encodes a 177 amino acid protein (19.0 kDa) with a putative transmembrane domain between residues 32 and 52. There is a potential signal peptide cleavage site at residue 54 and several possible motifs for phosphorylation and myristylation. Rabbit antisera raised against a synthetic peptide from the C terminus of the j 13L ORF identified proteins of 25-27 kDa in cells infected with a recombinant vaccinia virus expressing the j 13L ORF, in ASFV-infected cells and in purified extracellular ASF virions. In ASFV-infected cells the jl3L protein was expressed late during infection and exhibited size variation (25-27 kDa) between the different ASFV strains. Nucleotide sequence analysis of the gene in these strains showed that these size differences were due to variation in the number and sequence of tandemly repeated amino acid repeats. Although ASFV-infected animals made antibodies to the jl3L protein, no protection was observed when pigs were vaccinated with a recombinant vaccinia virus expressing the j 13L ORF.

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“…Experimental vaccines based on the use of an inactivated virus or virus subunits have failed to induce solid protective immunity [3,4,5,6]. Homologous protective immunity does develop in swine surviving infections with moderately virulent or attenuated ASFV isolates [7,8,9,10].…”

Section: Introductionmentioning

confidence: 99%

Association of the Host Immune Response with Protection Using a Live Attenuated African Swine Fever Virus Model

Carlson

O’Donnell

Alfano

et al. 2016

Viruses

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African swine fever (ASF) is a lethal hemorrhagic disease of swine caused by a double-stranded DNA virus, ASF virus (ASFV). There is no vaccine to prevent the disease and current control measures are limited to culling and restricting animal movement. Swine infected with attenuated strains are protected against challenge with a homologous virulent virus, but there is limited knowledge of the host immune mechanisms generating that protection. Swine infected with Pretoriuskop/96/4 (Pret4) virus develop a fatal severe disease, while a derivative strain lacking virulence-associated gene 9GL (Pret4Δ9GL virus) is completely attenuated. Swine infected with Pret4Δ9GL virus and challenged with the virulent parental virus at 7, 10, 14, 21, and 28 days post infection (dpi) showed a progressive acquisition of protection (from 40% at 7 dpi to 80% at 21 and 28 dpi). This animal model was used to associate the presence of host immune response (ASFV-specific antibody and interferon (IFN)-γ responses, or specific cytokine profiles) and protection against challenge. With the exception of ASFV-specific antibodies in survivors challenged at 21 and 28 dpi, no association between the parameters assessed and protection could be established. These results, encompassing data from 65 immunized swine, underscore the complexity of the system under study, suggesting that protection relies on the concurrence of different host immune mechanisms.

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The immunological response of pigs and Guinea pigs to antigens of African swine fever virus

Cited by 50 publications

References 9 publications

African Swine Fever Virus

African Swine Fever Virus

African swine fever virus gene j13L encodes a 25-27 kDa virion protein with variable numbers of amino acid repeats

Association of the Host Immune Response with Protection Using a Live Attenuated African Swine Fever Virus Model

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