The Immunohistopathology of Glomerular Antigens THE GLOMERULAR BASEMENT MEMBRANE, COLLAGEN, AND ACTOMYOSIN ANTIGENS IN NORMAL AND DISEASED KIDNEYS

Scheinman, Jon I.; Fish, Alfred J.; Michael, Alfred F.

doi:10.1172/jci107858

Cited by 60 publications

(38 citation statements)

References 24 publications

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“…Mesangial regions of human glomeruli also stain with an antimyosin antibody (44,45), although there is not any evidence that this is specific for smooth muscle cells. Our studies demonstrate that under pathological conditions (i.e., with mesangial proliferation) mesangial cells express a-smooth muscle actin and desmin, both muscle specific markers, thus providing additional evidence for a phenotypic similarity between smooth muscle cells and mesangial cells.…”

Section: Resultsmentioning

confidence: 99%

Expression of smooth muscle cell phenotype by rat mesangial cells in immune complex nephritis. Alpha-smooth muscle actin is a marker of mesangial cell proliferation.

Johnson¹,

Iida²,

Alpers³

et al. 1991

J. Clin. Invest.

417

263

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Mesangial cell proliferation is common in glomerulonephritis but it is unclear if proliferation is associated with any in vivo alteration in phenotype. We investigated whether mesangial cells acquire smooth muscle-like characteristics in a rat model of mesangial proliferative nephritis induced with antibody to the Thy-i antigen present on mesangial cells. At day 3 glomeruli displayed de novo immunostaining for a-smooth muscle actin in a mesangial pattern, correlating with the onset of proliferation, and persisting until day 14. An increase in desmin and vimentin in mesangial regions was also noted. Immunoelectron microscopy confirmed that the actin-positive cells were mesangial cells, and double immunolabeling demonstrated that the smooth muscle actin-positive cells were actively proliferating. Northern analysis of isolated glomerular RNA confirmed an increase in a and #/-y actin mRNA at days 3 and 5. Complement depletion or platelet depletion prevented or reduced proliferation, respectively; these maneuvers also prevented smooth muscle actin and actin gene expression. Studies of five other experimental models of nephritis confirmed that smooth muscle actin expression is a marker for mesangial cell injury. Thus, mesangial cell proliferation in glomerulonephritis in the rat is associated with a distinct phenotypic change in which mesangial cells assume smooth muscle cell characteristics. (J. Clin. Invest. 1991. 87:847-858.)

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Section: Resultsmentioning

confidence: 99%

Expression of smooth muscle cell phenotype by rat mesangial cells in immune complex nephritis. Alpha-smooth muscle actin is a marker of mesangial cell proliferation.

Johnson¹,

Iida²,

Alpers³

et al. 1991

J. Clin. Invest.

417

263

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“…It is recently speculated that monocytes can also present the phagocytic activity against immune complexes and/or macromolecules even after monocytes have entered mesangium. Sheinman et al (1978) reported the findings of marked decrease in both actomyosin and fibronectin in mesangium in progressive mesangial proliferative glomerulonephritis, indicating that the glomerular mesangium function has close relation to the progression of renal disease. These findings and speculation reported in the recent literature well consist with the results observed in the present study.…”

mentioning

confidence: 97%

Monocyte function in idiopathic nephrotic syndrome in childhood.

Nagata

TAKARASHI

Waga

et al. 1981

Tohoku J. Exp. Med.

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“…Recently, discrete collagen and glycoprotein antigens have been demonstrated in site-specific arrays along the glomerular capillary wall and within the mesangium (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18). It has not been clear whether these antigens are intrinsic components of the GBM that correspond to isolated moieties or are altered and perhaps lost during isolation by either sonication or detergent extraction.…”

mentioning

confidence: 99%

“…Uterine smooth muscle actomyosin was characterized by ATPase activity and gel electrophoresis and the antiserum by immunodiffusion and absorption (9,25). These antibodies and antisera were localized with fluorescein-isothiocyanatelabeled (FITC) goat anti-rabbit IgG (N. L. Cappel Laboratories, Cochranville, PA).…”

mentioning

confidence: 99%

Preservation of mesangium and immunohistochemically defined antigens in glomerular basement membrane isolated by detergent extraction.

Houser¹,

Scheinman²,

Basgen³

et al. 1982

J. Clin. Invest.

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A B S T R A C T To define the characteristics of isolated glomerular basement membrane (GBM), immunohistochemical and morphometric analyses have been carried out on rat and human tissues. Site-specific arrays of antigens were identified in detergent-isolated GBM in a distribution similar to that observed in intact kidney. In the human, fibronectin, procollagen IV, and collagen V were observed along the internal aspect of GBM continuous with antigenic sites in the mesangium. Another array of antigens was identified in the GBM but not within the mesangium-Goodpasture's antigen, bovine lens capsule type IV collagen, and amyloid P component. In addition, sites reactive with rabbit antiserum to laminin were present on both sides of the lamina densa as well as within the mesangial region. Actomyosin, a presumed mesangial cell antigen persisted in the mesangium of isolated GBM. Mesangial matrix was identified in detergent-isolated GBM in an amount equivalent to that present in intact glomeruli. Sonicated GBM contained the same antigens but it was not possible to quantitate the amount of mesangial material by immunofluorescence or morphometric analysis. The thickness of the lamina densa was greater in sonicated and detergent-treated rat GBM preparations than in native rat kidney. These studies demonstrated that isolated GBM is heterogeneous with respect to its antigenic constituents and in addition contains mesangial matrix, which is morphologically and immunohistochemically distinct from peripheral GBM.

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The Immunohistopathology of Glomerular Antigens THE GLOMERULAR BASEMENT MEMBRANE, COLLAGEN, AND ACTOMYOSIN ANTIGENS IN NORMAL AND DISEASED KIDNEYS

Cited by 60 publications

References 24 publications

Expression of smooth muscle cell phenotype by rat mesangial cells in immune complex nephritis. Alpha-smooth muscle actin is a marker of mesangial cell proliferation.

Expression of smooth muscle cell phenotype by rat mesangial cells in immune complex nephritis. Alpha-smooth muscle actin is a marker of mesangial cell proliferation.

Monocyte function in idiopathic nephrotic syndrome in childhood.

Preservation of mesangium and immunohistochemically defined antigens in glomerular basement membrane isolated by detergent extraction.

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