The immunohistochemical localization of pregnancy-specific β1-glycoprotein in postimplantation rat trophoblast

Macpherson, Trevor A.; Zheng, Shiyu; Kunz, Heinz W.; Ghani, Ahmed M.; Gill, Thomas J.

doi:10.1016/s0143-4004(85)80020-5

Cited by 8 publications

(2 citation statements)

References 17 publications

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“…Whereas Hau and his colleagues (1980) developed homologous murine models, other authors reported heterologous primate (Inaba et al 1987) and non-primate models (MacPherson et al 1985). Frequently, immunohistochemical heterologous studies were based on application of concentrated rabbit anti-htmaan placental proteins (1/10-1/20 dilution), that is, conditions conducive to high background staining (Inaba et al 1987).…”

Section: Discussionmentioning

confidence: 99%

“…By contrast, distribution of human SP1 (Bohn 1986) and PAPP-A immunoreactivity was restricted to mammals (Sinosich 1986). However, the presence or absence of immunologically related human SP1 analogs in rodents remains a contentious issue (Lin and Halbert 1978, Bohn and Ronnebeger 1973, Tatarinov et al 1980, MacPherson et al 1985. These studies clearly demonstrate the limitations of reporting apparent immunoreactivity in antibody excesses.…”

Section: Introductionmentioning

confidence: 91%

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A baboon model for pregnancy-associated antigens (PAPP-A, PP5, PP14)

Sinosich

Pope²,

Pope³

et al. 1990

Arch Gynecol Obstet

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By radioimmunoassays established on human derived antigens, PAPP-A, PP5 and PP14 immunoreactivity was detected in placental extracts and blood of pregnant baboons. None of the serial dilution curves suggested parallelism between respective human and baboon samples. Based on slopes of regressed logit-log transformed binding data, PAPP-A demonstrated the greatest degree of interspecies immunological crossreactivity. PP14 showed the least conservation of antigenic determinants. Physicochemical characterization on heparin, zinc chelate and bovine thrombin affinity matrices could not distinguish human from baboon-derived antigens. As in the human, baboon PAPP-A and PP5 were not detected in blood of male or non-pregnant animals. PP14 was detected in baboon follicular fluid, and only PP5 immunoreactivity was measured in culture media of baboon embryos. Of the three antigens, PAPP-A was detected in pregnant baboons at about 61 days gestation, that is, 4 weeks before PP5 and PP14. With the exception of PP14 which attained peak concentration at 118 days of pregnancy, PAPP-A and PP5 concentrations were greatest at term. In conjunction with physicochemical and immunological criteria, these physiological kinetics clearly support a role for developing a baboon model to serve for further studies into feto-maternal signals, particularly antigens such as PAPP-A and PP5.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 91%

A baboon model for pregnancy-associated antigens (PAPP-A, PP5, PP14)

Sinosich

Pope²,

Pope³

et al. 1990

Arch Gynecol Obstet

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Spatiotemporal expression of pregnancy‐specific glycoprotein gene rnCGM1 in rat placenta

Rebstock

Lucas

Weiß

et al. 1993

Developmental Dynamics

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As a basis towards a better understanding of the role of the pregnancy-specific glycoprotein (PSG) family in the maintenance of pregnancy, detailed investigations are described on the expression of a recently identified rat PSG gene (rnCGMl) at the mRNA and protein levels. Using specific oligonucleotide primers, rnCGMl transcripts were identified after reverse transcription, polymerase chain reaction, and hybridization with a radiolabelled, internal oligonucleotide. Transcripts were only found in significant amounts in placenta. In situ hybridization visualized rnCGM1 transcripts at day 14 post coitum (P.c.), in secondary trophoblast giant cells and in the spongiotrophoblast. Only those secondary giant cells lining the maternal decidua were positive. In contrast, primary giant cells did not contain rnCGMl mRNA. At day 18 P.c., rnCGMl transcripts were almost exclusively detectable in the spongiotrophoblast. No rnCGMl transcripts were found in rat embryos of these two developmental stages. Rabbit antisera were generated against the amino-terminal immunoglobulin variable-like domain and against a synthetic peptide containing the last 13 carboxy-terminal amino acids of rnCGMI. Both antisera recognized a 124 kDa protein in day 18 rat placental extracts as identified by Western blot analysis. The anti-peptide antiserum recognized a 116 kDa protein in the serum of a 14 day p.c. pregnant rat that is absent from the sera of non-pregnant females. Taken together, these results confirm exclusive expression of rnCGMl in the rat trophoblast, but unlike human PSG, negligible or no expression is found in other organs, such as fetal liver or salivary glands, indicating a more specialized function of rnCGM1. Its spatiotemporal expression pattern is conducive with a potential role of PSG in protecting the fetus against the maternal immune system andlor in regulating the invasive growth of trophoblast cells. 0 1993 Wiley-Liss, Inc.

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