The immunohistochemical identification of hormone‐producing cells in formalin‐fixed, paraffin‐embedded human pituitary tissue

Ac, Kruseman; Gt, Bots; Lindeman, E

doi:10.1002/path.1711170306

Cited by 18 publications

(2 citation statements)

References 19 publications

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“…Evidence is already avail able that these methods may have a similar impact in relation to the diagnosis of other neoplasms (e.g. glial specific proteins in gliomas [4], polypeptide hormones in pituitary adenomas [7], carcinoembryonic antigen in colonic carcinomas [6]), and that we stand at the threshold of a new era in the histological diagnosis of neoplasia.…”

Section: The Immunohistological Approach: Immunoperoxidase Methods Apmentioning

confidence: 99%

Immunohistological Approach to Tumor Diagnosis

Cr¹

1978

Oncology

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Immunoperoxidase methods permit the demonstration of a variety of antigens in routinely processed tissues. The morphological detail obtained by this procedure is comparable to orthodox haematoxylin and eosin stained sections, and so offers the diagnostic pathologist the opportunity of simultaneously identifying normal and neoplastic cells both by their morphological characteristics and by their antigenic constitution. The application of this method to a study of malignant lymphomas has contributed to current re-appraisal of classification criteria for this group of neoplasms. It is anticipated that, as specific antisera against a range of different tissue antigens become available, immunoperoxidase methods will have a similar impact upon the re-definition of morphologic criteria related to other neoplasms.

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Section: The Immunohistological Approach: Immunoperoxidase Methods Apmentioning

confidence: 99%

Immunohistological Approach to Tumor Diagnosis

Cr¹

1978

Oncology

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“…Human postmortem pituitary tissue contains immunocytochemically identifiable somatotrophs [21]. To deter mine if such tissue was capable of promoting growth of the hypox rat, glands from 2 postmortem cases were individual ly minced and six ~ I mm' pieces loaded/hollow fiber.…”

Section: Other Applications Of Pituitary Hollow Fiber Unitsmentioning

confidence: 99%

Pituitary Hollow Fiber Units in vivo and in vitro

Hymer¹,

Wilbur

Page

et al. 1981

Neuroendocrinology

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A new technique is described for the study of mammalian pituitary cells in vivo and in vitro. The method involves encapsulation of freshly trypsinized rat, sheep or human pituitary cells in XM-50 Amicon hollow fibers followed by their intracranial implantation into hypophysectomized rats. These pituitary fiber units promoted recipient growth for ∼3 weeks before weight gains plateaued. Body composition analyses showed that significant quantities of protein, fat and ash accounted for the weight gain. Morphological study of the capsule contents 7–39 days postimplantation revealed the presence of intact somatotrophs and corticotrophs. The hollow fibers may provide an immunologically privileged site by virtue of the fact that the 50,000 dalton pores making up the lumen surface permit pituitary hormones to diffuse from the capsule, but theoretically do not permit immunoglobulin molecules to penetrate the capsule. Growth of hypox rats receiving capsules containing allogeneic rat pituitary cells, sheep cells or pieces of human postmortem pituitary support this concept. Furthermore, rats implanted with human PRL adenoma cells had detectable quantities of circulating hPRL 100 days post-implantation. It is suggested that the pituitary hollow fiber units function when they come in contact with a ventricular surface of a hypox animal. With these units, it will be possible to study function of the same group of pituitary cells in vitro and in vivo.

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Use of immunohistochemical and morphologic methods for the identification of human growth hormone-producing pituitary adenomas

Kruseman

Bots

Lindeman

et al. 1976

Cancer

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Indirect immunoperoxidase and immunofluorescent techniques were used for the detection of growth hormone in routinely processed human pituitary adenomas. With the immunoperoxidase method, distinct immunoreactive cells could be demonstrated in 15 adenomas; all except for one were associated with acromegaly. Immunofluorescence was less useful because of autofluorescence due to the fixation. In histologic stainings, the immunoreactive cells were acidophyl. With immunohistochemistry and electron microscopy, densely granulated and sparsely granulated adenomas could be distinguished. Sparse granulation was associated with an expanded rough-surfaced endoplasmic reticulum and Golgi complex. Clinically, the sparsely granulated adenomas were characterized both by active hormone secretion and aggressive local growth. It is concluded that the immunoperosidase method is of value for functional classification of pituitary adenomas. For information about secretory activity we suggest that electron micrographs of adenoma cells be examined.

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The immunohistochemical identification of hormone‐producing cells in formalin‐fixed, paraffin‐embedded human pituitary tissue

Cited by 18 publications

References 19 publications

Immunohistological Approach to Tumor Diagnosis

Immunohistological Approach to Tumor Diagnosis

Pituitary Hollow Fiber Units in vivo and in vitro

Use of immunohistochemical and morphologic methods for the identification of human growth hormone-producing pituitary adenomas

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