The immunoglobulin of the brown trout, Salmo trutta and its concentration in the serum of antigen‐stimulated and non‐stimulated fish

Abstract

Immunoglobulin production in the primary and secondary immune response of brown trout to keyhole limpet haemocyanin has been investigated including the effect of dose size, route and number of injections, and the use of adjuvant. Antibody activity was found in the first fraction from Sephadex G200 and in the second from Sepharose 6B. Trout immunoglobulin had pa-yl electrophoretic mobility, and S,,, of 16.7 and an approximate molecular weight of 670 000 daltons. It was sensitive to dithiothreitol and stable at 56" C for 30 min. Immunoglobulin concentrations were measured by single radial immunodiffusion with a specific rabbit antiserum. Sera from non-injected trout had a mean immunoglobulin level of 7.3k0.3 mg ml-l which accounted for 10% of the total serum protein. Phosphate buffered saline-injected controls contained 6.7+ 0.2. In fish given a single injection the mean concentration ranged from 7.5 to 12.9 and in those given more than one injection from 12.6 to 16.8. The use of adjuvant resulted in higher immunoglobulin concentrations. Neither dose nor route had any significant effect on the primary response. However, in the secondary response the intramuscular route resulted in significantly increased immunoglobulin production.

Changes in agglutinating antibody titers and serum proteins of immunized eels

“…The activity was lost after treatment with 2-ME, a substance used to reduce the disulfide bounds of immunoglobulins. This result agrees with reports on other fish where the activity of the immunoglobulins was completely abolished by 2-ME treatment (Evelyn 1971, Clem & McLean 1975, Ingram & Alexander 1979. Studies on the separation and purification of other bony fish serum antibodies demonstrated that only the IgM class occurs (Ambrosius et al 1982).…”

Association of plasma IgM with body size, histopathologic changes, and plasma chemistries in adult Pacific herring Clupea pallas

“…This is the first report of plasma IgM levels in Pacific herring. Serum IgM concentrations have been reported for several other species: Oncorhynchus mykiss, 0.6 to 9.3 mg rnl-' ) and 3.3 mg ml-' (Olesen & Jsrgensen 1986); Salmo trutta, 7.3 mg ml-' (Ingram & Alexander 1979); Oncorhynchus keta, 0.63 to 0.72 mg rnl-' (Kobayashi et al 1982); and Scophthalmus maximus, 3.35 to 11.14 mg ml-' (Estevez et al 1995). The IgM component of total serum protein in fish has been reported as 6.4 % (Mochida et al 1994), 10 % (Ingram & Alexander 1979), and 7.5 to hoferi infection, a variety of nonspecific histologic 18% (Estevez et al 1995).…”

The immunoglobulin of colored carp, Cyprinus carpio, against Aeromonas hydrophila.

“…1 (KusuDA et al, 1981). Furthermore, in immunized brown trout the immunoglobulin concentration increased and ranged from 7.5 to 12.9 % after one injection, and from 12.6 to 16.8% after two or three injections (INGRAM et al, 1979).…”