The immunoglobulin constant region contributes to affinity and specificity

Selective intrathecal enrichment of G1m1‐positive B cells in multiple sclerosis

“…Another possibility is that yet unidentified MS‐associated polymorphisms in the variable heavy chain (VH) region are inherited together with the G1m polymorphisms. Alternatively, the G1m1 and/or G1m17 polymorphisms could indirectly cause allosteric changes in the VH region influencing the binding affinity of the IgG1 BCR 17. Finally, it has been shown that the G1m allotypes could affect the binding affinities of soluble IgG1 for the neonatal Fc receptor (FcRn) 18.…”

Combined glyco- and protein-Fc engineering simultaneously enhance cytotoxicity and half-life of a therapeutic antibody

“…60,61 Conversely, the Fc region has been shown to contribute to affinity and specificity of mAbs. 62 For all these reasons, we didn't focus our study on only one variant but several combinations that are stored and documented in a database, to be able to propose Fc variants that fit best for a given mAb. Preliminary results on two other specificities confirm that several combinations are directly transferrable to other mAbs, resulting in increased half-life in hFcRn mice (data not shown).…”

Thermodynamic Analysis of the Binding of 2F5 (Fab and Immunoglobulin G Forms) to Its gp41 Epitope Reveals a Strong Influence of the Immunoglobulin Fc Region on Affinity

“…In contrast, numerous authors have reported that both variable (V) and constant (C) Ig regions contribute to the binding affinity and specificity of antibodies (11,(17)(18)(19)(20)(21)(22)(23). Most of these studies have focused on affinity differences between distinct Ig isotypes.…”