The Immunoexpression of Heparanase 2 in Normal Epithelium, Intraepithelial, and Invasive Squamous Neoplasia of the Cervix

Marques, Renato Moretti; Focchi, Gustavo Rubino de Azevedo; Theodoro, Therésè Rachell; Castelo, Adauto; Pinhal, Maria Aparecida da Silva; Nicolau, Sérgio Mancini

doi:10.1097/lgt.0b013e3182422c69

Cited by 11 publications

(9 citation statements)

References 19 publications

(10 reference statements)

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“…Likewise, HPSE2 was suggested as a good tumor marker, better than HPSE, for the first time only in 2008 in colorectal carcinoma in comparison with normal mucosa with sensitivity of 98% and specificity of 100% using immunohistochemistry technique[ 18 ]. Moreover, HPSE2 was also associated with the diagnosis of ovarian cancer with accuracy of 78%[ 41 , 42 ] and recently with better outcomes in patients with gastric cancer[ 43 ].…”

Section: Discussionmentioning

confidence: 99%

The Profile of Heparanase Expression Distinguishes Differentiated Thyroid Carcinoma from Benign Neoplasms

et al. 2015

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IntroductionThe search for a specific marker that could help to distinguish between differentiated thyroid carcinoma and benign lesions remains elusive in clinical practice. Heparanase (HPSE) is an endo-beta-glucoronidase implicated in the process of tumor invasion, and the heparanase-2 (HPSE2) modulates HPSE activity. The aim of this study was to evaluate the role of heparanases in the development and differential diagnosis of follicular pattern thyroid lesions.MethodsHPSE and HPSE2 expression by qRT-PCR, immunohistochemistry evaluation, western blot analysis and HPSE enzymatic activity were evaluated.ResultsThe expression of heparanases by qRT-PCR showed an increase of HPSE2 in thyroid carcinoma (P = 0.001). HPSE activity was found to be higher in the malignant neoplasms than in the benign tumors (P<0.0001). On Western blot analysis, HPSE2 isoforms were detected only in malignant tumors. The immunohistochemical assay allowed us to establish a distinct pattern for malignant and benign tumors. Carcinomas showed a typical combination of positive labeling for neoplastic cells and negative immunostaining in colloid, when compared to benign tumors (P<0.0001). The proposed diagnostic test presents sensitivity and negative predictive value of around 100%, showing itself to be an accurate test for distinguishing between malignant and benign lesions.ConclusionsThis study shows, for the first time, a distinct profile of HPSE expression in thyroid carcinoma suggesting its role in carcinogenesis.

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Section: Discussionmentioning

confidence: 99%

The Profile of Heparanase Expression Distinguishes Differentiated Thyroid Carcinoma from Benign Neoplasms

et al. 2015

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“…The recent work identifying homozygosity at the Pv11 microsatellite as a risk factor for disease in CSL subsequently identified HPSE2 as a potential gene of interest. Increased labelling of HPA2 with increasing severity of disease is noted in human cervical tissues [73]. Preliminary studies in tissues from CSL identified differential labelling of HPA2 according to genotype but did not find an association between quantity of labelling and severity of disease [11].…”

Section: Urogenital Carcinoma In the California Sea Lion Exhibits Simmentioning

confidence: 99%

Common cancer in a wild animal: the California sea lion ( Zalophus californianus ) as an emerging model for carcinogenesis

Browning

Gulland

Hammond

et al. 2015

Phil. Trans. R. Soc. B

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Naturally occurring cancers in non-laboratory species have great potential in helping to decipher the often complex causes of neoplasia. Wild animal models could add substantially to our understanding of carcinogenesis, particularly of genetic and environmental interactions, but they are currently underutilized. Studying neoplasia in wild animals is difficult and especially challenging in marine mammals owing to their inaccessibility, lack of exposure history, and ethical, logistical and legal limits on experimentation. Despite this, California sea lions ( Zalophus californianus ) offer an opportunity to investigate risk factors for neoplasia development that have implications for terrestrial mammals and humans who share much of their environment and diet. A relatively accessible California sea lion population on the west coast of the USA has a high prevalence of urogenital carcinoma and is regularly sampled during veterinary care in wildlife rehabilitation centres. Collaborative studies have revealed that genotype, persistent organic pollutants and a herpesvirus are all associated with this cancer. This paper reviews research to date on the epidemiology and pathogenesis of urogenital carcinoma in this species, and presents the California sea lion as an important and currently underexploited wild animal model of carcinogenesis.

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“…26 In ovarian neoplasms, the intensity of HPSE2 expression was not different, comparing benign and malignant tumors, but all nonneoplastic samples were negative for HPSE2. 27 In gastric cancer, the overexpression of HPSE2 was associated with better prognoses, and it was considered a possible biomarker for a favorable evolution.…”

Section: Discussionmentioning

confidence: 90%

Immunohistochemical Expression of Heparanases 1 and 2 in Benign Tissue and in Invasive Neoplasia of the Endometrium: A Case-Control Study

Filho¹,

Focchi²,

Theodoro

et al. 2015

Int J Gynecol Cancer

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Heparanase 1 is more intensely expressed in the glandular tissue of high-grade compared with type I carcinomas. Heparanase 2 is more intensely expressed in the glandular tissue of cancer than in nonneoplastic endometrium, whereas the HPSE2 expression in the stromal tissue is higher in the nonneoplastic controls compared with the group of patients with cancer mainly in the secretory endometrium. This suggests that HPSE2 might be stimulated by progesterone, with a possible antineoplastic role, antagonist to HPSE1, to be further investigated.

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The Immunoexpression of Heparanase 2 in Normal Epithelium, Intraepithelial, and Invasive Squamous Neoplasia of the Cervix

Cited by 11 publications

References 19 publications

The Profile of Heparanase Expression Distinguishes Differentiated Thyroid Carcinoma from Benign Neoplasms

The Profile of Heparanase Expression Distinguishes Differentiated Thyroid Carcinoma from Benign Neoplasms

Common cancer in a wild animal: the California sea lion ( Zalophus californianus ) as an emerging model for carcinogenesis

Immunohistochemical Expression of Heparanases 1 and 2 in Benign Tissue and in Invasive Neoplasia of the Endometrium: A Case-Control Study

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