The Immunity Protection of Central Nervous System Induced by Pseudorabies Virus DelgI/gE/TK in Mice

Xu, Lei; Wei, Jianfeng; Zhao, Jun; Lee, Feng-Qin; Nie, Mincai; Xu, Zhiwen; Zhou, Yuancheng; Zhu, Ling

doi:10.3389/fmicb.2022.862907

Cited by 10 publications

(4 citation statements)

References 23 publications

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“…The vaccine demonstrated promising results in preliminary studies conducted in mice, effectively shielding them from the lethal assault of the PRV XJ variant strain. It inhibited the neural damage and inflammation induced by the PRV XJ variant strain, activating innate immunity in the brain and intestinal tract (Xu et al, 2022). The results of this study showed that PRV XJ del gI/gE/ TK vaccine effectively protects piglets from PRV XJ variant strain by activating intestinal immunity and has a strong protective effect.…”

Section: Introductionmentioning

confidence: 69%

“…Several studies have demonstrated the efficacy of PRV genedeleted vaccines in protecting piglets from variant PRV strains and preventing neurological and respiratory symptoms (Xu et al, 2022;Jiang et al, 2023). However, little research has focused on the effects of PRV on the gut and whether gene-deleted vaccines can counteract these negative effects.…”

Section: Discussionmentioning

confidence: 99%

“…MW893682), identified as a variant strain, was isolated and characterized by our laboratory. Subsequently, our laboratory successfully constructed a PRV XJ del gI/gE/TK variant, in which the gI, gE, and TK genes were deleted (Xu et al, 2022). To propagate all viruses, the PK-15 cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% (v/v) fetal bovine serum.…”

Section: Virus and Cellsmentioning

confidence: 99%

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The immunity protection of intestine induced by pseudorabies virus del gI/gE/TK in piglets

Zhang,

Xu,

Tao

et al. 2024

Front. Microbiol.

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Compared to the classical strain of Pseudorabies virus (PRV), the PRV variant exhibits stronger transmissibility and pathogenicity, causing immense disasters for the global pig industry. Based on this variant, our laboratory has preliminarily constructed a modified pseudorabies virus with deletions in the gE/gI/TK genes. In this study, the protective efficacy of PRV XJ del gI/gE/TK against piglet intestinal damage was evaluated. The results demonstrated that piglets immunized with PRV XJ del gI/gE/TK exhibited alleviated intestinal damage caused by the PRV XJ variant strain. This included reduced viral load, suppressed inflammation, and maintenance of intestinal structure and function. Additionally, PRV XJ del gI/gE/TK also strongly activated the innate immune response in the intestines, increasing the expression of antiviral factor mRNA and the secretion of SIgA to counteract the attack of the PRV XJ variant strain. Our study indicates that PRV XJ del gI/gE/TK can inhibit intestinal damage caused by PRV XJ variant strain and activate the innate immune response in the intestines.

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Section: Introductionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Section: Virus and Cellsmentioning

confidence: 99%

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The immunity protection of intestine induced by pseudorabies virus del gI/gE/TK in piglets

Zhang,

Xu,

Tao

et al. 2024

Front. Microbiol.

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“…This results in high fever, increased intracranial pressure, cerebral edema, and eventually coma or death. Pathological manifestations include vacuolar degeneration, neuronophagy, and inflammatory cell infiltration ( Xu et al, 2022 ). With the exception of pigs, almost all mammals infected with PRV develop severe neurological symptoms and die.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome and metabolome analysis reveals PRV XJ delgE/gI/TK protects intracranially infected mice from death by regulating the inflammation

Xu,

Zhang,

Tao

et al. 2024

Front. Microbiol.

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Pseudorabies virus can cause inflammation in the central nervous system and neurological symptoms. To further investigate the protective mechanism of PRV XJ delgE/gI/TK in the central nervous system, an intracranial PRV-infection mice model was developed. The results demonstrated that immunization with PRV XJ delgE/gI/TK successfully prevented death caused by PRV-intracranial infection. Subsequently, the brains were collected for transcriptome and metabolome analysis. GO and KEGG enrichment analysis indicated that the differentially expressed genes were primarily enriched in pathways such as TNF, NOD-like receptor, JAK–STAT, MAPK, IL-17 and apoptosis signaling. Metabolomics analysis revealed that the differential metabolites were mainly associated with pathways such as fatty acid degradation, arachidonic acid metabolism, linoleic acid metabolism and unsaturated fatty acid biosynthesis. The combined analysis of metabolites and differentially expressed genes revealed a strong correlation between the differential metabolites and TNF, PI3K, and MAPK signaling pathways. Anti-inflammatory metabolites have been shown to inhibit the inflammatory response and prevent mouse death caused by PRV infection. Notably, when glutathione was injected intracranially and dihydroartemisinin was injected intraperitoneally, complete protection against PRV-induced death in mice was observed. Moreover, PRV activates the PI3K/AKT signaling pathway. In conclusion, our study demonstrates that PRV XJ delgE/gI/TK can protects intracranially infected mice from death by regulating various metabolites with anti-inflammatory functions post-immunization.

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Characterization and Evaluation of the Pathogenicity of a Natural Gene-Deleted Transmissible Gastroenteritis Virus in China

Dai

Luo

et al. 2023

Transboundary and Emerging Diseases

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Porcine transmissible gastroenteritis virus is the major pathogen that causes fatal diarrhea in newborn piglets. In this study, a TGEV strain was isolated from the small intestine of diarrhea piglets in Sichuan Province, China, and designated SC2021. The complete genomic sequence of TGEV SC2021 was 28561 bp, revealing a new natural deletion TGEV strain. Based on phylogenetic analyses, TGEV SC2021 belonged to the Miller cluster and was closely related to CN strains. The newborn piglets orally challenged with TGEV SC2021 showed typical watery diarrhea. In addition, macro and micropathological changes in the lungs and intestines were observed. In conclusion, we isolated a new natural deletion virus strain and confirmed that the virus strain has high pathogenicity in newborn piglets. Moreover, macroscopic and microscopic lesions were observed in the lungs and intestines of all TGEV SC2021-infected piglets. In summary, we isolated a new natural deletion TGEV strain and demonstrated that the natural deletion strain showed high pathogenicity in newborn piglets. These data enrich the diversity of TGEV strains and help us to understand the genetic evolution and molecular pathogenesis of TGEV.

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The Immunity Protection of Central Nervous System Induced by Pseudorabies Virus DelgI/gE/TK in Mice

Cited by 10 publications

References 23 publications

The immunity protection of intestine induced by pseudorabies virus del gI/gE/TK in piglets

The immunity protection of intestine induced by pseudorabies virus del gI/gE/TK in piglets

Transcriptome and metabolome analysis reveals PRV XJ delgE/gI/TK protects intracranially infected mice from death by regulating the inflammation

Characterization and Evaluation of the Pathogenicity of a Natural Gene-Deleted Transmissible Gastroenteritis Virus in China

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