The immune response of a warm water fish orange-spotted grouper (Epinephelus coioides) infected with a typical cold water bacterial pathogen Aeromonas salmonicida is AhR dependent

Huang, Lixing; Qi, Weilu; Zuo, Yanfei; Alias, Siti Aisyah; Xü, Wei

doi:10.1016/j.dci.2020.103779

Cited by 15 publications

(21 citation statements)

References 40 publications

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“…Finally, the slides were dyed with 1% crystal violet for 3 min and then observed with a microscope and imaged with a digital camera (magnification, ×1,000). The number of bacteria was quantified from the image using IPwin software (3 slides with 20 visual fields per slide) ( Ryckaert et al., 2010 ; Isla et al., 2014 ; Zhang et al., 2018b ; Huang et al., 2020a ).…”

Section: Methodsmentioning

confidence: 99%

“…TRIzol reagent (Invitrogen, Carlsbad, CA, USA) was used for total RNA extraction from bacterial cells, as directed by the manufacturer. Reverse transcription was carried out with A Reverse Aid Mu-MLV cDNA synthesis kit (TransGen Biotech Co., Ltd., Beijing, China) from 2.0 mg total RNA, as instructed by the manufacturer ( Ge et al., 2020 ; Huang et al., 2020a ; Xu et al., 2021 ).…”

Section: Methodsmentioning

confidence: 99%

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Effect of Ferredoxin Receptor FusA on the Virulence Mechanism of Pseudomonas plecoglossicida

Wang

Lin

et al. 2022

Front. Cell. Infect. Microbiol.

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Pseudomonas plecoglossicida is an aerobic Gram-negative bacterium, which is the pathogen of “Visceral white spot disease” in large yellow croaker. P. plecoglossicida is a temperature-dependent bacterial pathogen in fish, which not only reduces the yield of large yellow croaker but also causes continuous transmission of the disease, seriously endangering the healthy development of fisheries. In this study, a mutant strain of fusA was constructed using homologous recombination technology. The results showed that knockout of P. plecoglossicida fusA significantly affected the ability of growth, adhesion, and biofilm formation. Temperature, pH, H2O2, heavy metals, and the iron-chelating agent were used to treat the wild type of P. plecoglossicida; the results showed that the expression of fusA was significantly reduced at 4°C, 12°C, and 37°C. The expression of fusA was significantly increased at pH 4 and 5. Cu2+ has a significant inducing effect on the expression of fusA, but Pb2+ has no obvious effect; the expression of fusA was significantly upregulated under different concentrations of H2O2. The expression of the fusA gene was significantly upregulated in the 0.5~4-μmol/l iron-chelating agent. The expression level of the fusA gene was significantly upregulated after the logarithmic phase. It was suggested that fusA included in the TBDR family not only was involved in the transport of ferredoxin but also played important roles in the pathogenicity and environment adaptation of P. plecoglossicida.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Effect of Ferredoxin Receptor FusA on the Virulence Mechanism of Pseudomonas plecoglossicida

Wang

Lin

et al. 2022

Front. Cell. Infect. Microbiol.

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“…A. salmonicida (SRW-OG1) was isolated from naturally infected Epinephelus coioides in our laboratory ( Huang et al., 2020a ). After artificial infection, the strain was identified as a pathogenic strain and confirmed as A. salmonicida by biochemical identification and 16S rRNA sequencing.…”

Section: Methodsmentioning

confidence: 99%

“…To investigate the mechanism underlying the virulence regulation at different temperatures, the genomics and transcriptomics analysis on A. salmonicida SRW-OG1 have been carried out. Type II secretion system was found in the genome of A. salmonicida SRW-OG1 ( Huang et al., 2020a ), while the expressions of tatA , tatB , and tatC , which belong to Type II secretion system (T2SS), were greatly affected by temperature stress.…”

Section: Introductionmentioning

confidence: 99%

The temperature-dependent expression of type II secretion system controls extracellular product secretion and virulence in mesophilic Aeromonas salmonida SRW-OG1

Chen

Cai

et al. 2022

Front. Cell. Infect. Microbiol.

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Aeromonas salmonicida is a typical cold water bacterial pathogen that causes furunculosis in many freshwater and marine fish species worldwide. In our previous study, the pathogenic A. salmonicida (SRW-OG1) was isolated from a warm water fish, Epinephelus coioides was genomics and transcriptomics analyzed. Type II secretion system was found in the genome of A. salmonicida SRW-OG1, while the expressions of tatA, tatB, and tatC were significantly affected by temperature stress. Also, sequence alignment analysis, homology analysis and protein secondary structure function analysis showed that tatA, tatB, and tatC were highly conservative, indicating their biological significance. In this study, by constructing the mutants of tatA, tatB, and tatC, we investigated the mechanisms underlying temperature-dependent virulence regulation in mesophilic A. salmonida SRW-OG1. According to our results, tatA, tatB, and tatC mutants presented a distinct reduction in adhesion, hemolysis, biofilm formation and motility. Compared to wild-type strain, inhibition of the expression of tatA, tatB, and tatC resulted in a decrease in biofilm formation by about 23.66%, 19.63% and 40.13%, and a decrease in adhesion ability by approximately 77.69%, 80.41% and 62.14% compared with that of the wild-type strain. Furthermore, tatA, tatB, and tatC mutants also showed evidently reduced extracellular enzymatic activities, including amylase, protease, lipase, hemolysis and lecithinase. The genes affecting amylase, protease, lipase, hemolysis, and lecithinase of A. salmonicida SRW-OG1 were identified as cyoE, ahhh1, lipA, lipB, pulA, HED66_RS01350, HED66_RS19960, aspA, fabD, and gpsA, which were notably affected by temperature stress and mutant of tatA, tatB, and tatC. All above, tatA, tatB and tatC regulate the virulence of A. salmonicida SRW-OG1 by affecting biofilm formation, adhesion, and enzymatic activity of extracellular products, and are simultaneously engaged in temperature-dependent pathogenicity.

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“…E. fasciatus which is adaptable to conditions the environment of the lagoon waters to coral reef areas (Astuti et al, 2016). E. coioides, large and medium sized warmwater marine fish which are well known in tropical and subtropical waters, is an economically important and widely cultivated fish in South China (Huang et al, 2020). Fish the mud grouper (Epinephelus coioides) has a large head, wide mouth, and body small scales.…”

Section: Figure 1 Grouper Phylogenetics Serranidae In Indonesia Collected From Local Fish Marketsmentioning

confidence: 99%

Phylogenetic analysis of the grouper family (Serranidae) from various local markets in Indonesian waters using COI (cytochrome oxidase I)

et al. 2021

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Grouper fish (Serranidae) is a type of fish found in Indonesian waters. However, not many people have conducted further research on phylogenetics based on COI (Cytochrome Oxidase I). This study aims to explain the phylogeny of grouper fish from the Serranidae family based on COI (Cytochrome Oxidase I). This research is a literature study. The research samples were grouper fish from Lombok, Karimunjawa, Lampung, Kendari, Madura, Tanakeke, and Numfor. The research instrument was a grouper-type observation sheet and an observation sheet for the results of the MEGA 7 application test. The data were analyzed using qualitative descriptive analysis. The results showed that the level of kinship between the species tested was very close, including Epinephelus areolatus, E. merra, E. fasciatus, E. longispinis, E. coioides, E. ongus, and E. coeruleopunctatus with all genetic distance averages type. 0.02. The conclusion of this study is that in general the species relationships found are several species found in the same location with similar morphology and diet.

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The immune response of a warm water fish orange-spotted grouper (Epinephelus coioides) infected with a typical cold water bacterial pathogen Aeromonas salmonicida is AhR dependent

Cited by 15 publications

References 40 publications

Effect of Ferredoxin Receptor FusA on the Virulence Mechanism of Pseudomonas plecoglossicida

Effect of Ferredoxin Receptor FusA on the Virulence Mechanism of Pseudomonas plecoglossicida

The temperature-dependent expression of type II secretion system controls extracellular product secretion and virulence in mesophilic Aeromonas salmonida SRW-OG1

Phylogenetic analysis of the grouper family (Serranidae) from various local markets in Indonesian waters using COI (cytochrome oxidase I)

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