The immune response in patients with cutaneous leishmaniasis and the influence of zinc supplementation

Vitamins A and D and Zinc Affect the Leshmanicidal Activity of Canine Spleen Leukocytes

“…Vitamin D deficiency has been associated with the progression of CanL [ 19 ], and low serum levels of vitamin A [ 20 ] and Zn [ 27 ] have been detected in patients with leishmaniasis. On this basis, we quantitated the serum levels of these nutrients in the study dogs and observed low levels of retinol ( Figure 1 A) and Zn ( Figure 1 C) in the CanL group compared to healthy dogs, while there were higher levels of 25(OH)VD 3 in the CanL group ( Figure 1 B).…”

“…After reconstitution, the nutrients were successively diluted in the RPMI-1640 medium, from the order of milligrams to nanograms. Three different concentrations for each nutrient were tested in a previous pilot study, based on the literature [ 20 , 26 , 27 ] and including variations of ±50% of the reference values, and the percentage of cell death by apoptosis was titrated by flow cytometry using the Guava Nexin kit (Millipore) according to the manufacturer’s instructions. In the cytometry analysis, we considered the percentage of cells in late apoptosis that were positive for PE-conjugated annexin and 7AA-D collected from the FL2 and FL3 channels of the flow cytometer, respectively.…”

An appraisal of the scientific current situation and new perspectives in the treatment of cutaneous leishmaniasis

“…Another interesting approach includes the use of adjuvants to help the immune system. In this regard, Guzman-Rivero et al found that zinc supplementation improved the immune response in patients with CL treated with antimony (Guzman-Rivero et al, 2015). Farajzadeh et al compared the efficacy of intralesional injection of zinc sulphate 2 % solution with intralesional Glucantime® in the treatment of acute CL, concluding that both treatments were equally effective (Farajzadeh et al, 2016a).…”