The immune enhancement of a novel soy lecithin/β-glucans based adjuvant on native Neospora caninum tachyzoite extract vaccine in mice

Mansilla, Florencia Celeste; Franco-Mahecha, Olga Lucía; Lavoria, María Ángeles; Moore, D.P.; Giraldez, Adrian Nicolas; Iglesias, Marcela; Wilda, Maximiliano; Capozzo, Alejandra Victoria

doi:10.1016/j.vaccine.2011.12.007

Cited by 16 publications

(30 citation statements)

References 44 publications

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“…The other adjuvant was aluminium hydroxide (“ALUM”, Rehydragel HPA. Chemtrade, Toronto, Ontario, Canada, Reheis ® , New York, USA) and was used at 4%, as previously reported …”

Section: Methodsmentioning

confidence: 99%

Fusion of foreign T‐cell epitopes and addition of TLR agonists enhance immunity against Neospora caninum profilin in cattle

Mansilla¹,

Quintana

Cardoso

et al. 2016

Parasite Immunology

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We demonstrated recently that immunization with recombinant Neospora caninum profilin (rNcPRO) induces limited protection and a regulatory T-cell response in mice. The aim of this study was to evaluate the immune response elicited by rNcPRO in cattle and assess a strategy to enhance its immunogenicity, combining the addition of T-cell epitopes and immune modulators. We developed a chimeric recombinant profilin fused to functional T-cell epitopes present in the N-terminal sequence of vesicular stomatitis virus (VSV) glycoprotein G (rNcPRO/G). Groups of three cattle were immunized with two doses (2 weeks apart) of rNcPRO or rNcPRO/G formulated with alum hydroxide or a nanoparticulated soya-based adjuvant enriched with Toll-like receptor (TLR) 2 and TLR9 agonists, aimed to tackle the MyD88 pathway (AVECplus). rNcPRO induced only a primary immune response (IgM mediated), while antibodies in rNcPRO/G-vaccinated animals switched to IgG1 after the booster. The vaccine formulated with rNcPRO/G and AVECplus improved the production of systemic IFN-γ and induced long-term recall B-cell responses. Overall, our study provides data supporting the use of T-cell epitopes from VSV glycoprotein G and TLR agonists to enhance and modulate immunity to peptide antigens in bovines, particularly when using small proteins from parasites for which immune responses are usually feeble.

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“…The other adjuvant was aluminium hydroxide (“ALUM”, Rehydragel HPA. Chemtrade, Toronto, Ontario, Canada, Reheis ® , New York, USA) and was used at 4%, as previously reported …”

Section: Methodsmentioning

confidence: 99%

Fusion of foreign T‐cell epitopes and addition of TLR agonists enhance immunity against Neospora caninum profilin in cattle

Mansilla¹,

Quintana

Cardoso

et al. 2016

Parasite Immunology

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“…A new soy-lecithin adjuvant, Providean-AVEC®, was used by Mansilla et al (77) in the development of a vaccine against neosporosis. This adjuvant, which contains β-glucans from barley and yeast and a soluble extract from Chenopodium quinoa whole seeds, has demonstrated to completely limit the multiplication of the parasite causing the pathogenesis, to activate DCs and to enhance cellular responses when formulated with soluble N. caninum antigens (SNcAg) and s.c. administered to mice (77), encouraging its use against this parasite. In a follow-up study, Mansilla et al (78) demonstrated that the vaccine formulation containing Providean-AVEC® + SNcAg stimulated broad cellular and humoral immune responses against N. caninum in cattle.…”

Section: Adjuvants: the Black Box Of Immunology Being Openedmentioning

confidence: 99%

Promising Plant-Derived Adjuvants in the Development of Coccidial Vaccines

2019

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Coccidial parasites cause medical and veterinary diseases worldwide, frequently leading to severe illness and important economic losses. At present, drugs, chemotherapeutics and prophylactic vaccines are still missing for most of the coccidial infections. Moreover, the development and administration of drugs and chemotherapeutics against these diseases would not be adequate in livestock, since they may generate unacceptable residues in milk and meat that would avoid their commercialization. In this scenario, prophylactic vaccines emerge as the most suitable approach. Subunit vaccines have proven to be biologically safe and economically viable, allowing researchers to choose among the best antigens against each pathogen. However, they are generally poorly immunogenic and require the addition of adjuvant compounds to the vaccine formulation. During the last decades, research involving plant immunomodulatory compounds has become an important field of study based on their potential pharmaceutical applications. Some plant molecules such as saponins, polysaccharides, lectins and heat shock proteins are being explored as candidates for adjuvant/carriers formulations. Moreover, plant-derived immune stimulatory compounds open the possibility to attain the main goal in adjuvant research: a safe and non-toxic adjuvant capable of strongly boosting and directing immune responses that could be incorporated into different vaccine formulations, including mucosal vaccines. Here, we review the immunomodulatory properties of several plant molecules and discuss their application and future perspective as adjuvants in the development of vaccines against coccidial infections.

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“…The majority of recent work on the control of N. caninum has focused on vaccine approaches, including inactivated whole vaccines (Rojo-Montejo et al 2011), the use of sub-unit native (Moore et al 2011;Mansilla et al 2012) and recombinant N. caninum antigens (Hemphill et al 2013;Uchida et al 2013) and live vaccine approaches with a naturally attenuated isolate of N. caninum (Williams et al 2007;Rojo-Montejo et al 2013;Weber et al 2013). Prophylactic treatment with monensin (Vanleeuwen et al 2010(Vanleeuwen et al , 2011 to reduce the risk of abortion has also been investigated.…”

Section: Introductionmentioning

confidence: 99%

Control options forNeospora caninum– is there anything new or are we going backwards?

et al. 2014

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Recent work has highlighted and enumerated the economic annual losses due to Neospora caninum abortions worldwide, which should provide strong motivation for the control of bovine neosporosis. However, with the recent withdrawal from sale of the only commercially available vaccine, control options for N. caninum have become more restricted. While researchers continue to work on developing alternative efficacious vaccines, what are the control options presently available for the cattle industries? At the practical level, recommendations for 'Test-and-cull', or 'not breeding from seropositive dams' stand diametrically opposed to alternative options put forward that suggest a primary producer is better advised to keep those cows in the herd that are already seropositive, i.e., assumed to be chronically infected, and indeed those that have already aborted once. Treatment with a coccidiostat has been recommended as the only economically viable option, yet no such treatment has gained official, regulatory approval. Dogs are central to the life cycle of N. caninum and have repeatedly been associated with infection and abortions in cattle by epidemiological studies. Knowledge and understanding of that pivotal role should be able to be put to use in control programmes. The present review canvasses the relevant literature for evidence for control options for N. caninum (some of them proven, many not) and assesses them in the light of the authors' knowledge and experience with control of N. caninum.

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The immune enhancement of a novel soy lecithin/β-glucans based adjuvant on native Neospora caninum tachyzoite extract vaccine in mice

Cited by 16 publications

References 44 publications

Fusion of foreign T‐cell epitopes and addition of TLR agonists enhance immunity against Neospora caninum profilin in cattle

Fusion of foreign T‐cell epitopes and addition of TLR agonists enhance immunity against Neospora caninum profilin in cattle

Promising Plant-Derived Adjuvants in the Development of Coccidial Vaccines

Control options forNeospora caninum– is there anything new or are we going backwards?

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