The immonological impairment of vasopressin (AVP) neurons into paraventricular nuclei modifies AVP expression in suprachiasmatic nuclei

Rafaï, H.; Chapleur-Chateau, M.; Haumont-Pellegri, B.; Fernette, Brigitte; Angel, E.; Burlet, C.; Nicolas, J; Burlet, Arlette

doi:10.1016/0304-3940(95)12005-o

Cited by 3 publications

(2 citation statements)

References 27 publications

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“…A similar phenomenon has already been observed when AVP-MAb was injected into the PVN (Rafai et al, 1995). This decreased the AVP content of both pPVN and SCN, but increased that of mPVN.…”

Section: Discussionsupporting

confidence: 68%

Arginine vasopressin (AVP) depletion in neurons of the suprachiasmatic nuclei affects the AVP content of the paraventricular neurons and stimulates adrenocorticotrophic hormone release

et al. 1997

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Arginine vasopressin (AVP) produced in the hypothalamic suprachiasmatic nuclei (SCN) plays a role in establishing neuroendocrine rhythms and, in particular, in regulating the corticotrope axis rhythm. It has recently been shown that AVP from SCN inhibits corticosteroid release. In order to investigate the influence of suprachiasmatic AVP on the different peptidergic systems through the hypothalamus, SCN neurons containing AVP were functionally lesioned by using toxins associated with a cytotoxic monoclonal antibody (MAb) raised against AVP. Six days later, the AVP contents and AVP mRNA were measured in different hypothalamic and extrahypothalamic sites. Adrenocorticotrophic hormone (ACTH) concentration was also measured in plasma. Microinjection of the AVP-MAb/toxin mixture into SCN brought about a significant decrease in the AVP expression in SCN. This is demonstrated by the decrease in the AVP immunoreactive content (24%, P < 0.01) and the decrease of AVP hybridized mRNA (33%, P < 0.01). This points to the efficiency of the microinjection in decreasing the production of AVP in the injection area. Modifications of the AVP contents in the two subdivisions of the hypothalamic paraventricular nucleus (PVN) were also observed. AVP contents decreased in the parvocellular subdivision (pPVN); this is coherent with the AVP depletion in SCN since pPVN is the major site of the SCN hypothalamic efferences. AVP content and AVP mRNA increased in the magnocellular subdivision (mPVN); this also confirms the difference in AVP synthesis regulation according to the PVN subdivisions. The microinjection did not modify AVP expression in supraoptic nuclei or oxytocin (OT) immunoreactive content in the main hypothalamic OT containing sites. Plasma ACTH values were double (P < 0.02) the values measured under non-specific IgG treatment 10 hr after lights on. This probably resulted from the stimulation of the hypothalamo-pituitary-adrenal system since corticotrophin-releasing hormone (CRH) mRNA increased simultaneously by 24% (P < 0.05) in the PVN and the immunoreactive CRH content of the median eminence significantly decreased (26%, P < 0.05). Overall, our data confirm that AVP produced in the SCN inhibits the CRH-adrenocorticotrope axis in normal conditions, probably because of SCN projections of AVP neurons on the PVN.

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“…A similar phenomenon has already been observed when AVP-MAb was injected into the PVN (Rafai et al, 1995). This decreased the AVP content of both pPVN and SCN, but increased that of mPVN.…”

Section: Discussionsupporting

confidence: 68%

Arginine vasopressin (AVP) depletion in neurons of the suprachiasmatic nuclei affects the AVP content of the paraventricular neurons and stimulates adrenocorticotrophic hormone release

et al. 1997

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“…Rat brain was removed after decapitation 60 min after icv administration of PTHrP(1-34), PTH (1-34), PTHrP(7-37) (200 pmol/rat) or saline (1 l/rat) as previously described (24). Then, coronal hypothalamic slices containing the SON (400 m in thickness) were cut with a vibrating slicer.…”

Section: Northern Blot Analysesmentioning

confidence: 99%

Centrally Administered Parathyroid Hormone (PTH)-Related Protein(1–34) But Not PTH(1–34) Stimulates Arginine-Vasopressin Secretion and Its Messenger Ribonucleic Acid Expression in Supraoptic Nucleus of the Conscious Rats

et al. 1998

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It has been suggested that PTH-related protein (PTHrP) is an endogenous modulator of cardiovascular systems. We have reported that PTHrP(1-34), but not PTH(1-34), causes the release of argininevasopressin (AVP) from the supraoptic nucleus (SON) of the hypothalamus in vitro through a novel receptor distinct from the PTH/ PTHrP receptors (type I or type II) described previously. In this study, we have investigated the in vivo effects of PTHrP(1-34) on AVP secretion and its messenger RNA (mRNA) expression in the SON in conscious rats. Intracerebroventricular (icv) administration of PTHrP(1-34) resulted in an increase in plasma AVP concentration in a dose-dependent manner (0 -400 pmol/rat). The maximal effect was obtained at 15 min after icv administration of PTHrP(1-34). Neither PTHrP(7-34) nor PTH(1-34) had any effect on plasma AVP levels. PTHrP(1-34)-induced AVP secretion was antagonized by pretreatment with PTHrP(7-34) but not by that with PTH(1-34). In addition, in situ hybridization study revealed that AVP mRNA expression in the SON and paraventricular nucleus was significantly increased 30 min after icv administration of PTHrP(1-34) and reached a maximum at 180 min. Furthermore, in Northern blot analyses, AVP mRNA expression in the SON was increased to approximately a 2-fold of basal level by PTHrP(1-34). On the other hand, neither PTHrP(7-34) or PTH(1-34) had any effect on the mRNA expression. The PTHrP(1-34)-stimulated AVP mRNA expression was eliminated by pretreatment with PTHrP(7-34) but not with PTH(1-34). These results suggest that, in the central nervous system, PTHrP(1-34) is involved in AVP secretion through a novel receptor distinct from the PTH/PTHrP receptors reported previously, playing a role in the body water and electrolyte homeostasis. (Endocrinology 139: 383-388, 1998) P TH-RELATED protein (PTHrP) was identified in tumors associated with humoral hypercalcemia of malignancy (HHM) (1-3). Though PTHrP and PTH are expressed by different genes (1), the amino-terminal fragment of PTHrP and PTH interacts with a classical PTH/PTHrP receptor (type I) on bone and kidney because of the amino acid homology (2). These peptides also interact with a nonclassical PTH/PTHrP receptor (type II) on lymphocytes, insulinoma cells, keretinocytes, and squamous carcinoma cells.Since the demonstration of PTHrP protein and messenger RNA (mRNA) and its receptor protein in a variety of tissues associated with the cardiovascular system, it has been suggested that PTHrP functions as an autocrine/paracrine modulator in the cardiovascular tissues (4 -7). PTHrP acts on vascular smooth muscle, where it is produced (8, 9), as a vasorelaxant (10 -12). Iv administration of the amino-terminal PTHrP peptides induces hypotension in a specific manner (7,13,14). PTHrP also acts on heart muscle by increasing heart rate and contractility (4,10,15,16). Furthermore, vasoconstrictor substances, such as angiotensin II, endotheline, and catecholamines stimulate PTHrP mRNA expression in vascular smooth muscle cells (7, 13). These res...

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Role of arginine vasopressin and corticotropin-releasing factor in mediating alcohol-induced adrenocorticotropin and vasopressin secretion in male rats bearing lesions of the paraventricular nuclei

1997

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The immonological impairment of vasopressin (AVP) neurons into paraventricular nuclei modifies AVP expression in suprachiasmatic nuclei

Cited by 3 publications

References 27 publications

Arginine vasopressin (AVP) depletion in neurons of the suprachiasmatic nuclei affects the AVP content of the paraventricular neurons and stimulates adrenocorticotrophic hormone release

Arginine vasopressin (AVP) depletion in neurons of the suprachiasmatic nuclei affects the AVP content of the paraventricular neurons and stimulates adrenocorticotrophic hormone release

Centrally Administered Parathyroid Hormone (PTH)-Related Protein(1–34) But Not PTH(1–34) Stimulates Arginine-Vasopressin Secretion and Its Messenger Ribonucleic Acid Expression in Supraoptic Nucleus of the Conscious Rats

Role of arginine vasopressin and corticotropin-releasing factor in mediating alcohol-induced adrenocorticotropin and vasopressin secretion in male rats bearing lesions of the paraventricular nuclei

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