The immediate wound‐induced oxidative burst of <i>Saccharina latissima</i> depends on light via photosynthetic electron transport

McDowell, Ruth E.; Amsler, Margaret O.; Li, Qian; Lancaster, Jack R.; Amsler, Charles D.

doi:10.1111/jpy.12302

Cited by 19 publications

(8 citation statements)

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“…Local responses includes oxidative burst linked with cell wall reorganization or cell death 7 , 8 while systemic response, imply activation of defense related genes 9 , deposition of callose, accumulation of defensive proteins (mostly with enzymatic activity) and lectins 10 , 11 . These damage-induced changes are mediated by complex signaling networks, which include receptors, calcium (Ca 2+ ) influx, ATP release, kinase cascades, reactive oxygen species (ROS), reactive nitrogen species 12 and oxylipin signaling pathways 8 , 13 , 14 . Detailed studies revealed that mechanical injury is tightly linked with variation in electric potential 15 , 16 and chemical signals such as terpenes, methyl salicylate, methyl benzoate, ethylene, and especially jasmonic acid 16 .…”

Section: Introductionmentioning

confidence: 99%

Lipoxygenase in singlet oxygen generation as a response to wounding: in vivo imaging in Arabidopsis thaliana

Prasad

Sedlářová

Kale

et al. 2017

Sci Rep

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Wounding, one of the most intensive stresses influencing plants ontogeny and lifespan, can be induced by herbivory as well as by physical factors. Reactive oxygen species play indispensable role both in the local and systemic defense reactions which enable “reprogramming” of metabolic pathways to set new boundaries and physiological equilibrium suitable for survival. In our current study, we provide experimental evidence on the formation of singlet oxygen (1O2) after wounding of Arabidopsis leaves. It is shown that 1O2 is formed by triplet-triplet energy transfer from triplet carbonyls to molecular oxygen. Using lipoxygenase inhibitor catechol, it is demonstrated that lipid peroxidation is initiated by lipoxygenase. Suppression of 1O2 formation in lox2 mutant which lacks chloroplast lipoxygenase indicates that lipoxygenase localized in chloroplast is predominantly responsible for 1O2 formation. Interestingly, 1O2 formation is solely restricted to chloroplasts localized at the wounding site. Data presented in this study might provide novel insight into wound-induced signaling in the local defense reaction.

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Section: Introductionmentioning

confidence: 99%

Lipoxygenase in singlet oxygen generation as a response to wounding: in vivo imaging in Arabidopsis thaliana

Prasad

Sedlářová

Kale

et al. 2017

Sci Rep

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“…The production of ROS by an oxidative burst is an imperative element of the wound response in algae, plants, and animals [19]. As a response to wounding, plants release oligosaccharide cell wall fragments, which play an important role in the signaling cascade that initiates an intense, localized production of ROS [20][21][22].…”

Section: Manuscript To Be Reviewedmentioning

confidence: 99%

Peer Review #3 of "Real-time monitoring of superoxide anion radical generation in response to wounding: electrochemical study (v0.1)"

2017

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Background: The growth and development of plants are deleteriously affected by various biotic and abiotic stress factors. Wounding in plants is caused by exposure to environmental stress, mechanical stress, and via herbivory. Typically, oxidative burst in response to wounding is associated with the formation of reactive oxygen species, such as the superoxide anion radical (O 2 •−), hydrogen peroxide (H 2 O 2) and singlet oxygen; however, few experimental studies have provided direct evidence of their detection in plants. Detection of O 2 •− formation in plant tissues have been performed using various techniques including electron paramagnetic resonance spin-trap spectroscopy, epinephrineadrenochrome acceptor methods, staining with dyes such as tetrazolium dye and nitro blue tetrazolium (NBT), however, kinetic measurements have not been performed. In the current study, we provide evidence of O 2 •− generation and its kinetics in the leaves of spinach (Spinacia oleracea) subjected to wounding. Methods: Real-time monitoring of O 2 •− generation was performed using catalytic amperometry. Changes in oxidation current for O 2 •− was monitored using polymeric iron-porphyrin-based modified carbon electrodes (φ=1 mm) as working electrode with Ag/AgCl as the reference electrode. Result: The results obtained show a continuous generation of generation of O 2 •− for minutes after wounding, followed by a decline. The exogenous addition of superoxide dismutase, which is known to dismutate O 2 •− to H 2 O 2 , significantly suppressed the oxidation current. Conclusion: Catalytic amperometric measurements were performed using polymeric iron-porphyrin based modified carbon electrode. It is claimed to be a useful tool and a direct method for real-time monitoring and precise detection of O 2 •− in biological samples. It is claimed to bear a potential for its wide application in plant research for specific and sensitive detection of O 2 •− .

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“…The production of ROS by an oxidative burst is an imperative element of the wound response in algae, plants, and animals (McDowell et al, 2015). As a response to wounding, plants release oligosaccharide cell wall fragments, which play an important role in the signaling cascade that initiates an intense, localized production of ROS (Legendre et al, 1993; John et al, 1997; Stennis et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Real-time monitoring of superoxide anion radical generation in response to wounding: electrochemical study

Prasad

Kumar

Matsuoka

et al. 2017

PeerJ

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BackgroundThe growth and development of plants is deleteriously affected by various biotic and abiotic stress factors. Wounding in plants is caused by exposure to environmental stress, mechanical stress, and via herbivory. Typically, oxidative burst in response to wounding is associated with the formation of reactive oxygen species, such as the superoxide anion radical (O2•−), hydrogen peroxide (H2O2) and singlet oxygen; however, few experimental studies have provided direct evidence of their detection in plants. Detection of O2•− formation in plant tissues have been performed using various techniques including electron paramagnetic resonance spin-trap spectroscopy, epinephrine-adrenochrome acceptor methods, staining with dyes such as tetrazolium dye and nitro blue tetrazolium (NBT); however, kinetic measurements have not been performed. In the current study, we provide evidence of O2•− generation and its kinetics in the leaves of spinach (Spinacia oleracea) subjected to wounding.MethodsReal-time monitoring of O2•− generation was performed using catalytic amperometry. Changes in oxidation current for O2•− was monitored using polymeric iron-porphyrin-based modified carbon electrodes (φ = 1 mm) as working electrode with Ag/AgCl as the reference electrode.ResultThe results obtained show continuous generation of O2•− for minutes after wounding, followed by a decline. The exogenous addition of superoxide dismutase, which is known to dismutate O2•− to H2O2, significantly suppressed the oxidation current.ConclusionCatalytic amperometric measurements were performed using polymeric iron-porphyrin based modified carbon electrode. We claim it to be a useful tool and a direct method for real-time monitoring and precise detection of O2•− in biological samples, with the potential for wide application in plant research for specific and sensitive detection of O2•−.

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The immediate wound‐induced oxidative burst of Saccharina latissima depends on light via photosynthetic electron transport

Cited by 19 publications

References 88 publications

Lipoxygenase in singlet oxygen generation as a response to wounding: in vivo imaging in Arabidopsis thaliana

Lipoxygenase in singlet oxygen generation as a response to wounding: in vivo imaging in Arabidopsis thaliana

Peer Review #3 of "Real-time monitoring of superoxide anion radical generation in response to wounding: electrochemical study (v0.1)"

Real-time monitoring of superoxide anion radical generation in response to wounding: electrochemical study

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