The IL‐2/IL‐2‐Receptor Complex in the Maturation of Rat T‐Cell Progenitors

Varas, Alberto; Romo, T; Jiménez, Eva; Alonso, Luis Martı́nez; Vicente, Ángeles; Zapata, A.

doi:10.1155/1998/95861

Cited by 1 publication

(2 citation statements)

References 36 publications

(31 reference statements)

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“…The thymus provides a specialized microenvironment for T-cell development (78). Previous studies have shown that many components are involved in thymocyte development (72)(73)(74)79), including ECM integrin (80) and laminin (81,82). Laminin-2, a ligand of ␣-dystroglycan (83), and its integrin receptor VLA-6 interaction play an active role in thymocyte development by delivering cell survival and differentiation signals at specific stages of development in young adult mice.…”

Section: Discussionmentioning

confidence: 99%

“…Then we examined whether ␣-dystroglycan would play a role in thymocyte development. FTOC offers a model system in which the cellular interactions involved in fetal thymocyte development can be readily monitored and manipulated and have been used widely to study thymocyte development (72)(73)(74). However, blocking Ab was routinely used to explore the function of ␣-dystroglycan in certain tissues or cells (44,(47)(48)(49).…”

Section: Blocking ␣-Dystroglycan Decreased the Number Of Dp Thymocytesmentioning

confidence: 99%

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α‐Dystroglycan is involved in positive selection of thymocytes by participating in immunological synapse formation

Gong

Zhang

et al. 2008

FASEB j.

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Alpha-dystroglycan has been proved to be involved in lymphocyte activation by participating in immunological synapse (IS) formation. Considering the existence of IS formation in thymic development, we questioned whether alpha-dystroglycan was expressed in thymus and influenced thymic development. In this study, we demonstrated that alpha-dystroglycan was expressed on fetal thymocytes, especially on double-positive (DP, CD4(+)CD8(+)) cells. Blocking alpha-dystroglycan by treatment of fetal thymus organ culture (FTOC) with anti-alpha-dystroglycan antibody IIH6C4 decreased the number of DP cells compared with nontreated or isotype antibody controls. Down-regulation of alpha-dystroglycan by retroviruses carrying antisense cDNA of dystroglycan in reaggregate thymus organ culture (RTOC) further confirmed these results. Enhanced apoptosis of DP cells was observed after blocking alpha-dystroglycan. Interestingly, we found that blocking alpha-dystroglycan reduced IS formation between DP cells and thymic epithelial cells. Furthermore, blocking alpha-dystroglycan up-regulated CD95/CD95L expression and reduced Bcl-2 expression on DP cells in the developing thymus. Finally, the increase in the apoptosis of DP cells was associated with a consequent decrease in the positive selection, as indicated by the reduction of both ERK phosphorylation in DP cells and single-positive (SP, CD4(+) or CD8(+)) cell outcome. Altogether, these results indicated that alpha-dystroglycan was involved in positive selection of thymocytes by participating in the IS formation.

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