The IgE and basophil responses to Ara h 2 and Ara h 6 are good predictors of peanut allergy in children

Erp, Francine C. van; Knol, Edward F.; Pontoppidan, Bo; Meijer, Y.; Ent, Cornelis K. van der; Knulst, André C.

doi:10.1016/j.jaci.2016.06.041

Cited by 50 publications

(47 citation statements)

References 12 publications

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“…13,14 In contrast, in a recent study in children from our associated children hospital, we found a higher AUC for Ara h 2 compared to Ara h 6. 1 In our previous study with adult subjects, partly overlapping with the one from this study, we found very similar results for Ara h 2 and 6. 10 In all studies, however, the differences in discriminative ability between Ara h 2 and 6 remain small.…”

Section: Discussionsupporting

confidence: 89%

Specific IgE to peanut 2S albumin Ara h 7 has a discriminative ability comparable to Ara h 2 and 6

Blankestijn

Otten

Suer

et al. 2017

Clin Experimental Allergy

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Section: Discussionsupporting

confidence: 89%

Specific IgE to peanut 2S albumin Ara h 7 has a discriminative ability comparable to Ara h 2 and 6

Blankestijn

Otten

Suer

et al. 2017

Clin Experimental Allergy

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“…Ara h 2 and Ara h 6 have proven to be 2 of the most informative peanut allergens in the diagnosis of peanut allergy, as most patients have specific IgE against 1 or both allergens . In addition, the current study shows that 80% of the 15 peanut‐allergic patients studied were sensitized to 1 or multiple isoforms of a third recombinant 2S albumin member Ara h 7, mostly in combination with recombinant Ara h 2.0201 or Ara h 6.01.…”

Section: Discussionmentioning

confidence: 58%

“…To date, 17 peanut allergens (Ara h 1‐17) are known and most of them have been sequenced and cloned . Previous research indicated that screening for specific IgE against 2S albumins Ara h 2 and 6 to date is most effective in diagnosing peanut allergy, as the majority of peanut‐allergic patients have specific IgE against these allergens . Ara h 2 and Ara h 6 are both proteins belonging to the conglutin family, and Ara h 6 has approximately 53% sequence identity with Ara h 2 …”

Section: Introductionmentioning

confidence: 99%

2S protein Ara h 7.0201 has unique epitopes compared to other Ara h 7 isoforms and is comparable to 2S proteins Ara h 2 and 6 in basophil degranulation capacity

Hayen

Ehlers

Jäger

et al. 2018

Clin Experimental Allergy

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Summary Background Screening for specific IgE against 2S albumin proteins Ara h 2 and 6 has good positive predictive value in diagnosing peanut allergy. From the third 2S member Ara h 7, 3 isoforms have been identified. Their allergenicity has not been elucidated. Objective This study investigated the allergenicity of Ara h 7 isoforms compared to Ara h 2 and 6. Methods Sensitization of 15 DBPCFC‐confirmed peanut‐allergic patients to recombinant Ara h 2.0201, Ara h 6.01 and isoforms of recombinant Ara h 7 was determined by IgE immunoblotting strips. A basophil activation test (BAT) was performed in 9 patients to determine IgE‐cross‐linking capacities of the allergens. Sensitivity to the allergens was tested in 5 patients who were sensitized to at least 1 Ara h 7 isoform, by a concentration range in the BAT. 3D prediction models and sequence alignments were used to visualize differences between isoforms and to predict allergenic epitope regions. Results Sensitization to Ara h 7.0201 was most frequent (80%) and showed to be equally potent as Ara h 2.0201 and 6.01 in inducing basophil degranulation. Sensitization to Ara h 7.0201 together with Ara h 2.0201 and/or 6.01 was observed, indicating the presence of unique epitopes compared to the other 2 isoforms. Differences between the 3 Ara h 7 isoforms were observed in C‐terminal cysteine residues, pepsin and trypsin cleavage sites and 3 single amino acid substitutions. Conclusion & clinical relevance The majority of peanut‐allergic patients are sensitized to isoform Ara h 7.0201, which is functionally as active as Ara h 2.0201 and 6.01. Unique epitopes are most likely located in the C‐terminus or an allergenic loop region which is a known allergenic epitope region for Ara h 2.0201 and 6.01. Due to its unique epitopes and allergenicity, it is an interesting candidate to improve the diagnostic accuracy for peanut allergy.

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“…Thus, the BAT might be useful if clinical history, SPT and/or sIgE measurement are inconsistent or in addition an oral food challenge is not possible [42]. Furthermore, the use of recombinant allergens in the BAT might have added value in discriminating between clinically relevant and mere sensitization [44, 45] and should be considered in future studies.…”

Section: Discussionmentioning

confidence: 99%

Exploratory analysis of CD63 and CD203c expression in basophils from hazelnut sensitized and allergic individuals

Lötzsch

Dölle

Vieths

et al. 2016

Clin Transl Allergy

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BackgroundSensitization to hazelnut (HN) is frequent and requires clarification to determine whether this sensitization is clinically relevant. The aim of this study was to investigate basophil activation profiles in HN-sensitized and allergic subjects.MethodsBasophil activation was determined by flow cytometric analyses of CD63 and CD203c expression using several HN allergen concentrations. Depending on their clinical reaction pattern, an oral allergy symptom group (OAS, n = 20), a systemic reaction group (n = 12) and a sensitized group without clinical symptoms (n = 20) were identified. Additionally, 10 non-allergic and non-sensitized individuals served as controls.ResultsCD63 and CD203c expression differed between allergic (OAS and systemic group) and sensitized subjects. The HN concentration required to activate 30% of CD203c+ basophils [effective concentration (EC)30] was significantly higher in sensitized versus the allergic group (p = 0.0089). This was more pronounced when the basophil allergen threshold sensitivity (CD-sens) was calculated (CD63: p = 0.018; CD203c: p = 0.009).ConclusionOur data indicate that the basophil activation test may provide information to better distinguish between sensitized and allergic subjects if several allergen concentrations are considered. CD203c expression displayed a better discrimination compared to CD63; therefore, its diagnostic value might be superior compared with CD63.Electronic supplementary materialThe online version of this article (doi:10.1186/s13601-016-0134-7) contains supplementary material, which is available to authorized users.

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The IgE and basophil responses to Ara h 2 and Ara h 6 are good predictors of peanut allergy in children

Abstract: Thymic stromal lymphopoietin downregulates filaggrin expression by signal transducer and activator of transcription 3 (STAT3) and extracellular signal-regulated kinase (ERK) phosphorylation in keratinocytes.

Cited by 50 publications

References 12 publications

Specific IgE to peanut 2S albumin Ara h 7 has a discriminative ability comparable to Ara h 2 and 6

Specific IgE to peanut 2S albumin Ara h 7 has a discriminative ability comparable to Ara h 2 and 6

2S protein Ara h 7.0201 has unique epitopes compared to other Ara h 7 isoforms and is comparable to 2S proteins Ara h 2 and 6 in basophil degranulation capacity

Exploratory analysis of CD63 and CD203c expression in basophils from hazelnut sensitized and allergic individuals

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