The identification of urogastrone in serum, saliva, and gastric juice

Gregory, H.; Walsh, S. E.; Hopkins, Colin R.

doi:10.1016/0016-5085(79)90284-1

Cited by 97 publications

(13 citation statements)

References 14 publications

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“…Gregory et al (2) found that ir-EGF in serum appeared almost totally as a 100-200K form, probably corresponding to our 140K component. Gregory et al (2) found that ir-EGF in serum appeared almost totally as a 100-200K form, probably corresponding to our 140K component.…”

Section: Discussionmentioning

confidence: 56%

Characterization of Material With Epidermal Growth Factor Immunoreactivity in Human Serum and Platelets*

Pesonen

Viinikka

Myllylä

et al. 1989

The Journal of Clinical Endocrinology & Metabolism

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We investigated the molecular nature of the epidermal growth factor immunoreactivity (ir-EGF) in serum and platelets of normal subjects. In serum, ir-EGF appeared and increased during spontaneous blood coagulation, reaching a plateau in 2 h. The mean plateau measured by time-resolved immunofluorometric assay was 778 pg/mL [130 pmol/L; range, 465-1352 pg/mL (78-225 pmol/L)] for men (n = 66), and 774 pg/mL (129 pmoL/L; range, 521-1114 pg/mL (87-186 pmol/L)] for women (n = 33). Serum samples (n = 9), when examined under nonreducing conditions by high performance liquid chromatography, contained three mol wt components. Their approximate proportions averaged 56 +/- 6% (+/- SD) for a 140K component, 22 +/- 9% for a 67K component, and 22 +/- 6% for a component coeluting with the 6K EGF standard. The molecular size distribution of ir-EGF released from isolated platelets varied with the treatment of the platelets. After stimulation with a Ca2+ ionophore, the proportions of the 140K, 67K, and 6K components were 61 +/- 11%, 20 +/- 8%, and 17 +/- 4% (n = 5), respectively, as in serum. In addition, we found a small amount, (approximately 1%) of a 17K component. When platelets (n = 6) were ruptured by sonication and repeated freeze-thawing, the 67K component formed 53 +/- 7% of the total; the proportions of the 140K, 17K, and 6K components were 14 +/- 7%, 2.1 +/- 0.7%, and 31 +/- 4%, respectively. Under reducing conditions the 17K and 6K components remained intact, but part of the 140K component and all of the 67K component were cleaved to a 35-37K form. After protease digestion of the higher mol wt components, the ir-EGF was exclusively of the 6K form. The 67K and 6K components bound to the EGF receptor, whereas the 140K component bound inconsistently. We conclude that treatment of platelets with Ca2+ ionophore produces ir-EGF components similar to those found in incubated serum but different from those obtained by freeze-thawing. The 67K mol wt component appears to be the main storage form of EGF in platelets; at least two independent mechanisms appear to exist for EGF release.

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Section: Discussionmentioning

confidence: 56%

Characterization of Material With Epidermal Growth Factor Immunoreactivity in Human Serum and Platelets*

Pesonen

Viinikka

Myllylä

et al. 1989

The Journal of Clinical Endocrinology & Metabolism

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“…Epidermal growth factor was measured by a standard radio-immunoassay teeh-nique (11,22). Ant-body to EGF was a kind gift of Dr. H. GREGORY (ICI Pharmaceuticals, Macclesfield, England) and was used in a final dilution of 1 in 60,000,1'^' EGF for use as a tracer was prepared to the Iodogen method and purified using reverse phase high performance liquid chromatography.…”

Section: Measurement Ol Epidermai Growth Factormentioning

confidence: 99%

“…Epidermal growth factor (EGF) is a polypeptide hormone which is present in the ductal cells of salivary glands, including the parotid gland, in all species investigated (8)(9)(10)(11)(12)(13). In some species, the innervation of the ductal cells and the resultant alterations in salivary elec-trolyte composition are thought to be mediated by the sympathetic nervous system (14,15).…”

mentioning

confidence: 99%

Secretion of epidermal growth factor in parotid saliva in diabetic patients: role of autonomic innervation

Savage

Fisher

et al. 1990

J Oral Pathology Medicine

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Parotid saliva was collected over a 12-min period from 24 insulin dependent diabetic patients with varying degrees of autonomic neuropathy and 12 age and sex matched non-diabetic controls. Epidermal growth factor (EGF) concentrations in saliva were measured by radio-immunoassay. The EGF concentrations in diabetics with no autonomic neuropathy or with combined autonomic neuropathy were equivalent but secretion of EGF was significantly elevated at the 6- and 12-min periods of collection in diabetic patients with early or established autonomic neuropathy. It is postulated that when parasympathetic autonomic neuropathy is present a relative "over-activity" of the sympathetic innervation promotes release of salivary EGF. This sympathetic predominance may maintain salivary EGF concentration despite the elevated salivary flow and volume which is associated with parasympathetic autonomic neuropathy.

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“…In addition, the reports of Li et aL (1982) suggest that this peptide influences the development of rat colonic tumours in vivo. Urogastrone has been identified in a variety of human tissues and has close structural similarity to mEGF (Gregory, Walsh and Hopkins, 1979) and hence might be expected to have similar in vivo and in vitro effects on tumours.…”

Section: Introductionmentioning

confidence: 99%

Growth Factor‐induced Proliferative Responses of Human and Dmh‐induced Rat Colorectal Tumour Cell Lines

Maley¹,

Agrez²,

House³

1986

Aust J Exp Biol Med

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Summary. The proliferative response of three human and three 1,2-dimethylhydrazine (DMH)-induced rat colon tumour cell lines to mouse epidermal growth factor (mEGF) and human urogastrone was examined in two in vitro assays ('H-thymidine uptake in liquid microculture and clonogenicity in semi-solid medium). In the 'H-thymidine uptake assay, one of three rat and one of three human colon tumour cell lines, i.e., r237 and LIM1215, were stimulated by mEGF. In the clonogenic assay, mEGF stimulated the one clonogenic rat tumour cell line rll3, which did not respond in the 'H-thymidine uptake assay, and the human tumour cell line LIM1215. Urogastrone stimulated LIM1215 in both assay systems. The significance of these in vitro patterns of response to growth stimulatory factors is discussed.

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The identification of urogastrone in serum, saliva, and gastric juice

Cited by 97 publications

References 14 publications

Characterization of Material With Epidermal Growth Factor Immunoreactivity in Human Serum and Platelets*

Characterization of Material With Epidermal Growth Factor Immunoreactivity in Human Serum and Platelets*

Secretion of epidermal growth factor in parotid saliva in diabetic patients: role of autonomic innervation

Growth Factor‐induced Proliferative Responses of Human and Dmh‐induced Rat Colorectal Tumour Cell Lines

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