The identification of some water‐soluble arsenic species in the marine brown algae Fucus distichus

Harrington, Christopher F.; Ojo, A. A.; Lai, Vivan W.-M.; Reimer, Kenneth J.; Cullen, William R.

doi:10.1002/(sici)1099-0739(199712)11:12<931::aid-aoc627>3.3.co;2-6

Cited by 8 publications

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“…Arsenic peak at retention times 270-305 s and 305-360 s was identified as the AS2 and AS1, respectively. These are two separate hidden organoarsenic compounds in the Fucus distichus that have been previously identified [24]. The arsenic peak (194-275 s) of the tlc isolates SRA2 and SRB2 (Figures 6 and 8), as well as that for the filtrate sample SF (Figure 9), matched the retention times for a mixture of DMAE (major, 194-275s) and DMAA (minor, 230-275 s).…”

Section: Resultsmentioning

confidence: 99%

“…The peak area absorbance mode of the AAS was normally utilized with the HG-GC-AAS technique. The experimental and instrumental operating conditions for the HPLC-ICPMS analytical procedures have been previously reported [24]. The HPLC separation (on line with the ICPMS) was carried out on an Inertsil ODS-2 (250 × 4.6 mm id, GL Sciences, Japan) HPLC column using 10 mM tetraethylammonium hydroxide + 4 mM malonic acid + 0.1% methanol at pH 6.8 as the mobile phase.…”

Section: Hg-gc-aas and Hplc-icpms Analytical Proceduresmentioning

confidence: 99%

“…The seaweed extracts and chromatographic fractions or isolates were centrifuged to remove any suspended particulates prior to ion pair chromatography on the HPLC columns. Identification of arsenic compounds present in the seaweed samples was made by matching and comparing their retention times obtained from the HPLC-ICPMS chromatograms with those of known standards using spiked and/or unspiked arsenic compounds as well as four major arsenic species, namely, AB, DMAA, AS1 and AS2 in Figure 1 found in standard reference material, NIST 1566a Oyster Tissue [9,24].…”

Section: Hg-gc-aas and Hplc-icpms Analytical Proceduresmentioning

confidence: 99%

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Closed Anaerobic Biotransformation Products of Organoarsenic Compounds inFucus distichus

Ojo

Onasanya

2013

ISRN Environmental Chemistry

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The closed anaerobic decomposition extracts of Fucus distichus incubated with seawater and sediment, and without sediment as control, were subjected to extractions and isolation on Sephadex LH 20 and Cellulose Thin Layer Chromatography. The decomposition extracts and isolates were analyzed by using both the Hydride Generation Gas Chromatography Atomic Absorption Spectrometry (HG-GC-AAS) and High Performance Liquid Chromatography Inductively Coupled Plasma Mass Spectrometry (HPLC-ICPMS) to identify the arsenic species in the equilibrium mixtures of the seaweed and filtrates separately. In the methanol seaweed extract, equilibrium mixture of arsenosugars (AS) AS1 and AS2 and their biotransformation products of dimethylarsinoylethanol (DMAE) and dimethylarsinic acid (DMAA) were identified. In the methanol filtrate extract of the mixture, only DMAE and DMAA were identified. However, in the control methanol filtrate extract five organoarsenic species, AS1 and AS2, one unidentified hidden organoarsenic species, DMAE and DMAA were identified in the equilibrium mixture. This result confirmed that the hidden organoarsenic species in Fucus distichus, AS1 and AS2, and an unidentified organoarsenic compounds are biotransformed to only DMAE and DMAA under an anaerobic condition. This also suggests that DMAE and DMAA are strong intermediate candidates for the generation of arsenobetaine, from arsenoribosides in the marine food webs.

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Section: Resultsmentioning

confidence: 99%

Section: Hg-gc-aas and Hplc-icpms Analytical Proceduresmentioning

confidence: 99%

Section: Hg-gc-aas and Hplc-icpms Analytical Proceduresmentioning

confidence: 99%

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Closed Anaerobic Biotransformation Products of Organoarsenic Compounds inFucus distichus

Ojo

Onasanya

2013

ISRN Environmental Chemistry

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“…Arsenic is toxic to most organisms and in mammals it causes diverse effects, including cancer and cardiovascular disease (Hughs, 2002). In an aquatic environment, arsenic may occur in the inorganic forms of arsenate As(V) and arsenite As(III), and in organic forms, including arsenoribosides, arsenobetaine, monomethylarsonic acid, and dimethylarsinic acid (Harrington et al, 1997;Sohrin et al, 1997). In heavily-polluted estuaries along the Bohai Bay, North China, concentrations of total arsenic in water as high as 400 μg/L have been recorded (Meng et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

The influence of salinity on toxicological effects of arsenic in digestive gland of clam Ruditapes philippinarum using metabolomics

Wu²,

Liu

et al. 2013

Chin. J. Ocean. Limnol.

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Ruditapes philippinarum , a clam that thrives in intertidal zones of various salinities, is a useful biomonitor to marine contaminants. We investigated the infl uence of dilution to 75% and 50% of normal seawater salinity (31.1) on the responses of the digestive gland of R . philippinarum to arsenic exposure (20 μg/L), using nuclear magnetic resonance (NMR)-based metabolomics. After acute arsenic exposure for 48 h, salinity-dependent differential metabolic responses were detected. In normal seawater, arsenic exposure increased the concentrations of branched-chain amino acids, and of threonine, proline, phosphocholine and adenosine, and it decreased the levels of alanine, hypotaurine, glucose, glycogen and ATP in the digestive glands. Differential changes in metabolic biomarkers observed at lower salinity (~23.3) included elevation of succinate, taurine and ATP, and depletion of branched-chain amino acids, threonine and glutamine. Unique effects of arsenic at the lowest salinity (~15.6) included down-regulation of glutamate, succinate and ADP, and up-regulation of phosphocholine. We conclude that salinity infl uences the metabolic responses of this clam to arsenic.

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“…Due to the development of industry, arsenic was discharged to estuarine and coastal environments with an average amount more than 5000 tons/year in China from 2007 to 2010 [2]. In aquatic environment, arsenic may occur in different chemical forms, including inorganic forms of arsenite (As (III)) and arsenate (As (V)) and organic forms such as arsenoribosides, arsenobetaine, monomethylarsonic acid and dimethylarsinic acid [3]. In open ocean seawater, the concentration of total arsenic is usually around 1e3 mg/L [4].…”

Section: Introductionmentioning

confidence: 99%

Comparative investigations on the biological effects of As (III) and As (V) in clam Ruditapes philippinarum using multiple biomarkers

Wang

et al. 2015

Fish & Shellfish Immunology

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a b s t r a c tInorganic arsenic is a known pollutant with two chemical forms, arsenite (As (III)) and arsenate (As (V)), in marine environment. Clam Ruditapes philippinarum is an important fishery species along the Bohai coast. In this study, the biological effects induced by the two arsenic chemical forms (arsenite and arsenate) were compared using multiple biochemical indices in the digestive glands of clam R. philippinarum. The production of reactive oxygen species, antioxidant enzyme activities and metabolic responses exhibited that both As (III) and As (V) induced immune, oxidative and osmotic stresses in clam digestive glands. The differential metabolic biomarkers, histidine and taurine, indicated the differential responsive mechanisms in osmotic regulation in clam digestive glands. In addition, both arsenic treatments enhanced the anaerobiosis metabolism in clam digestive glands. Overall, this work illustrated that arsenite and arsenate induced similar biological effects in clams, which might be accounted for the biological transformation of arsenate to arsenite in clams.

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The identification of some water‐soluble arsenic species in the marine brown algae Fucus distichus

Cited by 8 publications

References 0 publications

Closed Anaerobic Biotransformation Products of Organoarsenic Compounds inFucus distichus

Closed Anaerobic Biotransformation Products of Organoarsenic Compounds inFucus distichus

The influence of salinity on toxicological effects of arsenic in digestive gland of clam Ruditapes philippinarum using metabolomics

Comparative investigations on the biological effects of As (III) and As (V) in clam Ruditapes philippinarum using multiple biomarkers

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