“…C values (Dudd, Evershed, & Gibson, 1999;Mottram et al, 1999;Copley et al, 2003;Spangenberg, 2004;Craig et al, 2005a;Spangenberg, Jacomet, & Schibler, 2006). FAME preparation is carried out on a portion of the total lipid extract, typically 1/4 (Copley et al, 2005c) beginning with hydrolysis with methanolic or ethanolic sodium or potassium hydroxide Spangenberg, Jacomet, & Schibler, 2006) to release fatty acid moieties from MAGs, DAGs, and TAGs, and from possibly other bound lipids .…”