The identification of genetic pathways involved in vascular adaptations after physical deconditioning <i>versus</i> exercise training in humans

Lammers, Gerwen; Duijnhoven, Noortje T.L. van; Hoenderop, Joost G. J.; Horstman, Astrid M.; Haan, A. de; Janssen, Thomas W J; Graaf, Mark J. J. de; Pardoel, Elisabeth M.; Verwiel, Eugène; Thijssen, Dick H. J.; Hopman, Maria T. E.

doi:10.1113/expphysiol.2012.068726

Cited by 18 publications

(11 citation statements)

References 64 publications

(69 reference statements)

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“…The training and immobilization data of the younger men was, respectively, cross-referenced with RT studies of 10 to 20 weeks (n = 58) 50-55 (File S4h) and immobilization/bed rest studies of 5 to 21 days (n = 42) 10, [56][57][58][59] (File S4i) in healthy, sedentary/active, lean/overweight, younger men. MetaMEx does not include retraining studies.…”

Section: Comparison With the Metamex Meta-analysismentioning

confidence: 99%

Recurrent training rejuvenates and enhances transcriptome and methylome responses in young and older human muscle

Blocquiaux

Ramaekers

Thienen

et al. 2020

Preprint

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ABSTRACTBackgroundThe interaction between the muscle methylome and transcriptome is understudied during ageing and periods of resistance training in young, but especially older adults. In addition, more information is needed on the role of retained methylome training adaptations in muscle memory to understand muscle phenotypical and molecular restoration after inactivity or disuse.MethodsWe measured CpG methylation (microarray) and RNA expression (RNA sequencing) in young (n = 5; age = 22 ± 2 yrs) and older (n = 6; age = 65 ± 5 yrs) vastus lateralis muscle samples, taken at baseline, after 12 weeks of resistance training, after training interruption (2 weeks of leg immobilization in young men, 12 weeks of detraining in older men) and after 12 weeks of retraining to identify muscle memory-related adaptations and rejuvenating effects of training.ResultsWe report that of the 427 differentially expressed genes with advanced age, 71 % contained differentially methylated (dm)CpGs in older versus young muscle. The more dmCpGs within a gene, the clearer the inverse methylation-expression relationship. Around 73 % of the age-related dmCpGs approached younger methylation levels when older muscle was trained for 12 weeks. A second resistance training period after training cessation increased the number of hypomethylated CpGs and upregulated genes in both young and older muscle. We found indication for an epi-memory within pro-proliferating AMOTL1 in young muscle and mechanosensing-related VCL in older muscle. For the first time, we integrate muscle methylome and transcriptome data in relation to both ageing and training/inactivity-induced responses and identify focal adhesion as an important pathway herein.ConclusionPreviously trained muscle is more responsive to training than untrained muscle at methylome and transcriptome level and recurrent resistance training can partially restore ageing-induced methylome alterations.

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Section: Comparison With the Metamex Meta-analysismentioning

confidence: 99%

Recurrent training rejuvenates and enhances transcriptome and methylome responses in young and older human muscle

Blocquiaux

Ramaekers

Thienen

et al. 2020

Preprint

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“…Although clearly a pathological state, given the acute onset involving SCI and that skeletal muscle itself is not typically injured (at least not initially with the onset of SCI), the denervation of skeletal muscle could be viewed as an archetypal physiologically mediated feedback mechanism attempting to match blood supply to a muscle that is progressively becoming deconditioned due to loss of (or very low) nerve activity to the muscle. In this context, it interesting to note that SCI studies report either no change or increased VEGF expression, even though capillary regression is occuring (Table ). Persistent elevation of VEGF has even been found following 25 months of denervation in the rat extensor digitorum longus muscle, despite a concurrent 9‐fold decrease in muscle capillarity .…”

Section: Introductionmentioning

confidence: 99%

Physiological Capillary Regression is not Dependent on Reducing VEGF Expression

Olfert

2016

Microcirculation

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Investigations into physiologically-controlled capillary regression report the provocative finding that microvessel regression occurs in the face of persistent elevation of skeletal muscle vascular endothelial growth factor-A (VEGF) expression. Thrombospondin-1 (TSP-1), a negative angiogenic regulator, is increasingly being observed to temporally correlate with capillary regression, suggesting that increased TSP-1 (and not reduction in VEGF per se) is needed to initiate, and likely regulate, capillary regression. Based on evidence being gleaned from physiologically-mediated regression of capillaries, it needs to be recognized that capillary regression (and perhaps capillary rarefaction with disease) is not simply the reversal of factors used to stimulate angiogenesis. Rather, the conceptual understanding that angiogenesis and capillary regression each have specific and unique requirements that are biologically constrained to opposite sides of the balance between positive and negative angioregulatory factors may shed light on why anti-VEGF therapies have not lived up to the promise in reversing angiogenesis and providing the cure that many had hoped toward fighting cancer. Emerging evidence from physiological controlled angiogenesis suggest that cases involving excessive or uncontrolled capillary expansion may be best treated by therapies designed to increase expression of negative angiogenic regulators, whereas those involving capillary rarefaction may benefit from inhibiting negative regulators (like TSP-1).

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“…A total number of 9975 persons were included. The overall median resting heart rate was 74 (interquartile range: [66][67][68][69][70][71][72][73][74][75][76][77][78][79][80]. Mean age has no important difference between males and females (P=0.79) but, resting heart rate was significantly higher in females (76.6 versus 71.4, P<0.001).…”

Section: Results and Conclusionmentioning

confidence: 99%

“…[2,58,74,75] Some other theories have been suggested about this matter, one is genetic factors that determine cardiovascular fitness and also energy expenditure. [76][77][78][79] Another theory explains that increased activity of sympathetic nervous system tone can lead to increase in HR and alter regulation of the parasympathetic on heart. Also, SNS overactivity stimulate hyperinsulinemia in accompaniment of insulin resistance.…”

Section: Discussionmentioning

confidence: 99%

Correlation of Resting Heart Rate with Anthropometric Factors and Serum Biomarkers in a Population-based Study: Fasa PERSIAN Cohort Study

Goorakani

Rahimabadi

Dehghan

et al. 2020

Preprint

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BackgroundThere is a positive association between raised resting heart rate (RHR), and all causes of mortality and shorter life expectancy. Several serum biomarkers and some anthropometric factors can affect the resting heart rate. This study aimed to investigate the determinants of resting heart rate in a large random sample of the Iranian population.Material and MethodsIt is a standardized, retrospective study and the subjects were chosen from the baseline survey of the Prospective Epidemiological Research Study in IrAN (PERSIAN) Fasa non-communicable disease cohort study. It was conducted from winter 2014 to summer 2019 and after obtaining informed consent from a random sample, all the eligible subjects were enrolled. All anthropometric factors and biologic laboratory factors were collected and analyzed by implement smoothly clipped absolute deviation (SCAD) linear regression and SCAD quantile regression. The comparisons between males and females were done via independent T-test.Results & ConclusionA total number of 9975 persons were included. The overall median resting heart rate was 74 (interquartile range:66-80). Mean age has no important difference between males and females (P=0.79) but, resting heart rate was significantly higher in females (76.6 versus 71.4, P<0.001). All anthropometric factors except wrist circumference were higher in females (P<0.05). Age has an adverse effect on resting heart rate and also, there was direct association between resting heart rate and systolic blood pressure and blood glucose. Alpha-blockers (coefficient=5.2) and Beta1-blockers (coefficient=-2.2) were the most effective drugs with positive and negative effects on resting heart rate respectively. Lower hemoglobin, obesity and more body mass index, and more low-density lipoprotein were associated with more resting heart rate.Continuing the monitoring of this sample via our cohort study and put to action multinational prospective researches with large sample sizes and long follow-ups can lead to more precise results and better scientific judgments.

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The identification of genetic pathways involved in vascular adaptations after physical deconditioning versus exercise training in humans

Cited by 18 publications

References 64 publications

Recurrent training rejuvenates and enhances transcriptome and methylome responses in young and older human muscle

Recurrent training rejuvenates and enhances transcriptome and methylome responses in young and older human muscle

Physiological Capillary Regression is not Dependent on Reducing VEGF Expression

Correlation of Resting Heart Rate with Anthropometric Factors and Serum Biomarkers in a Population-based Study: Fasa PERSIAN Cohort Study

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