The identification of bean mosaic, pea yellow mosaic and pea necrosis strains of bean yellow mosaic virus

Abstract

Twelve virus isolates from pea, broad bean, red clover and yellow lupin have been compared with the B25 strain of bean yellow mosaic virus (BYMV-B25), the E198 strain of pea mosaic virus (PMV-E198) and the pea necrosis virus (El 78), which were described earlier (Bos, 1970).On the basis of host ranges, symptoms and bean and pea varietal reactions most isolates could be classified into three groups, representatives of which did not differ appreciably serologically. These groups were considered to be typical bean yellow mosaic virus isolates (E212, L1, B25), pea yellow mosaic strain isolates of BYMV (E198, E204, Kow28) and pea necrosis strain isolates of BYMV (E197, E199, E221). From these results and from a survey of literature it is concluded that PMV is only a strain of BYMV.The pea necrosis virus (EI78), described earlier as a distinct entity, is still considered a different virus. A severe pea necrosis isolate (Kow14) resembled E178 in many respects and was also more distantly related serologically to the BYMV isolates tested. Four other virus isolates from pea and broad bean (E196, Vfl 5, Vf18 and Vf30) could not yet be identified. Lettuce mosaic virus (LMV) was found to be serologically rather closely related to BYMV.Results of cross-protection tests were erratic, and particle length measurements were no help in differentiating the strains and viruses studied.

Bean yellow mosaic, clover yellow vein, and pea mosaic are distinct potyviruses: evidence from coat protein gene sequences and molecular hybridization involving the 3? non-coding regions

“…For instance, some workers regard BYMV and PMV to be strains of the same virus [4,7,20,39], while others consider them as distinct viruses [3,27]. Similarly, BYMV and CYVV are considered by some workers to be strains of the same virus [8,9,20], while others have suggested that they are distinct potyviruses [3,19,27]. These conflicting assignments have been made on the basis of comparative biological and serological properties and from the results of molecular hybridization studies using randomly primed cDNA.…”

Virus Diseases of Faba Beans in Manitoba and Their Effects on Plant Growth and Yield

“…Fisher 1953) and red clover necrosis virus, isolated from peas, and was shown to be serologically related to BYMV (Zaumeyer and Goth 1963 (Bos et al 1974) in reacting more strongly with PMV than with BYMV antiserum.…”

Potyviruses, chaos or order?

“…Our earlier research had revealed the existence of strains of BYMV which were highly different pathologically, biological overlap of BYMV with CIYVV, and the existence of strains ofBYMV or of a separate virus especially adapted to faba bean [20]. We therefore compared representatives of these new faba bean isolates with isolates earlier identified as BYMV and CIYVV, for their biological and serological properties, and also employed virus-specific antibodies directed towards N-terminal parts of coat proteins [32].…”

“…Further studies in our laboratory in the early 1970's allowed the biological differentiation of bean mosaic, pea yellow mosaic, and pea necrosis strains of BYMV [20], the discrimination between the pea necrosis strain of BYMV and the pea necrosis virus [9], and the demonstration that the latter was ClYVV [21]. Through international comparison, pea leafroll mosaic virus was found to be one of the isolates of pea seed-borne mosaic virus which was described during the late 1960's more or less simultaneously with varying names in different parts of the world [41].…”