The identification of an age- and female-specific putative PBAN membrane-receptor protein in pheromone glands of Helicoverpa armigera: possible up-regulation by Juvenile Hormone

Rafaeli, Ada; Zakharova, T. S.; Lapsker, Zoria; Jurenka, Russell A.

doi:10.1016/s0965-1748(02)00264-3

Cited by 38 publications

(49 citation statements)

References 20 publications

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“…In some species it likely influences the circadian release of PBAN [58], in others it may up-regulate the PBAN-receptor protein in the pheromone gland [59]. In HvPG (but not HvLN) we found two JH binding proteins, EZ407156 and EZ407196: the latter was also found in BmPG and AsPG (see Figure 4).…”

Section: Resultsmentioning

confidence: 87%

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Transcriptome analysis of the sex pheromone gland of the noctuid moth Heliothis virescens

et al. 2010

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BackgroundThe chemical components of sex pheromones have been determined for more than a thousand moth species, but so far only a handful of genes encoding enzymes responsible for the biosynthesis of these compounds have been identified. For understanding the evolution of moth sexual communication, it is essential to know which genes are involved in the production of specific pheromone components and what controls the variation in their relative frequencies in the pheromone blend. We used a transcriptomic approach to characterize the pheromone gland of the Noctuid moth Heliothis virescens, an important agricultural pest, in order to obtain substantial general sequence information and to identify a range of candidate genes involved in the pheromone biosynthetic pathway.ResultsTo facilitate identifying sets of genes involved in a broad range of processes and to capture rare transcripts, we developed our majority of ESTs from a normalized cDNA library of Heliothis virescens pheromone glands (PG). Combining these with a non-normalized library yielded a total of 17,233 ESTs, which assembled into 2,082 contigs and 6,228 singletons. Using BLAST searches of the NR and Swissprot databases we were able to identify a large number of putative unique gene elements (unigenes), which we compared to those derived from previous transcriptomic surveys of the larval stage of Heliothis virescens. The distribution of unigenes among GO Biological Process functional groups shows an overall similarity between PG and larval transcriptomes, but with distinct enrichment of specific pathways in the PG. In addition, we identified a large number of candidate genes in the pheromone biosynthetic pathways.ConclusionThese data constitute one of the first large-scale EST-projects for Noctuidae, a much-needed resource for exploring these pest species. Our analysis shows a surprisingly complex transcriptome and we identified a large number of potential pheromone biosynthetic pathway and immune-related genes that can be applied to population and systematic studies of Heliothis virescens and other Noctuidae.

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Section: Resultsmentioning

confidence: 87%

“…In HvPG (but not HvLN) we found two JH binding proteins, EZ407156 and EZ407196: the latter was also found in BmPG and AsPG (see Figure 4). Finding these sequences specifically in the pheromone gland suggests that they may play a role in the binding of PBAN to its receptor [59]. …”

Section: Resultsmentioning

confidence: 99%

Transcriptome analysis of the sex pheromone gland of the noctuid moth Heliothis virescens

et al. 2010

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“…A study using a photoaffinity-biotinlabeled PBAN and pheromone glands of H. armigera demonstrated specific binding to a membrane-bound protein of Ϸ50 kDa (26). After accounting for the attached biotin-labeled PBAN, the apparent size of the receptor would be reduced to Ϸ45 kDa.…”

Section: Discussionmentioning

confidence: 99%

Identification of a G protein-coupled receptor for pheromone biosynthesis activating neuropeptide from pheromone glands of the moth Helicoverpa zea

Choi

Fuerst

Rafaeli

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

151

149

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Pheromone biosynthesis-activating neuropeptide (PBAN), a peptide produced by the subesophageal ganglion, is used by a variety of moths to regulate pheromone production. PBAN acts directly on pheromone gland cells by using calcium and cAMP as second messengers. We have identified a gene encoding a G proteincoupled receptor (GPCR) from pheromone glands of the female moth Helicoverpa zea. The gene was identified based on sequence identity to a group of GPCRs from Drosophila that are homologous to neuromedin U receptors in vertebrates. The full-length PBAN receptor was subsequently cloned, expressed in Sf9 insect cells, and shown to mobilize calcium in response to PBAN. This response was dose-dependent (EC 50 ‫؍‬ 25 nM) with a maximum response at 300 nM and a minimal observable response at 10 nM. Four additional peptides produced by the PBAN-encoding gene were also tested for activity, and it was determined that three had similar activity to PBAN and the other was slightly less active. Peptides belonging to the same family as PBAN, namely pyrokinins, as well as the vertebrate neuromedin U peptide also induced a calcium response. We have identified a GPCR for the PBAN͞pyrokinin family of peptides with a known function of stimulating pheromone biosynthesis in female moths. It is related to several receptors from insects (Drosophila and Anopheles) and to neuromedin U and ghrelin receptors from vertebrates.

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“…However, in view of our recent discovery of the presence of the PBAN-R gene transcript in male complexes (4) it is tempting to reexamine the possible functional role of PBAN in the regulation of fattyacid-derived components in this tissue. The discovery was surprising since in proteomic studies, using a binding assay, we were unable to detect the PBAN-R protein as detected in female pheromone glands (33) and thus, it may represent a nonfunctional gene. On the other hand, differential expression studies showed a temporal up-regulation of the PBAN-R gene transcript in male complexes of the two moth species, H. armigera and Bombyx mori, analogous to the up-regulation of the PBAN-R gene transcript in female pheromone glands of both species (34).…”

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confidence: 90%

Gene-silencing reveals the functional significance of pheromone biosynthesis activating neuropeptide receptor (PBAN-R) in a male moth

Bober

Rafaeli

2010

Proc. Natl. Acad. Sci. U.S.A.

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The role of pheromone biosynthesis activating neuropeptide (PBAN) in the regulation of pheromone biosynthesis of several female moth species is well elucidated, but its role in the males has been a mystery for over two decades since its discovery from both male and female central nervous systems. In previous studies we have identified the presence of the gene transcript for the PBAN-G-protein coupled receptor (PBAN-R) in Helicoverpa armigera male hair-pencil-aedaegus complexes (male complexes), a tissue structurally homologous to the female pheromone gland. Moreover, we showed that this transcript is up-regulated during pupal-adult development, analogous to its regulation in the female pheromone-glands, thereby indicating a likely functional gene. Here we argue in favor of PBAN's role in regulating the free fatty-acid components (myristic, palmitic, stearic, and oleic acids) and alcohol components (hexadecanol, cis-11 hexadecanol, and octadecanol) in male complexes. We demonstrate the diel periodicity in levels of male components, with peak titers occurring during the 7th-9th h in the scotophase, coincident with female pheromone production. In addition, we show significant stimulation of component levels by synthetic HezPBAN. Furthermore, we confirm PBAN's function in this tissue through knockdown of the PBAN-R gene using RNAi-mediated gene-silencing. Injections of PBAN-R dsRNA into the male hemocoel significantly inhibited levels of the various male components by 58%-74%. In conclusion, through gain and loss of function we revealed the functionality of the PBAN-R and the key components that are up-regulated by PBAN.aedaegus | hair-pencils | Helicoverpa armigera | male-produced components R eproductive behavior involves the integration of physiological and behavioral events that synchronize male and female encounters. Receptivity in female moths is broadcasted by the release of a blend of volatile sex-pheromones during specific times of the photoperiod when they assume typical calling behaviors.

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The identification of an age- and female-specific putative PBAN membrane-receptor protein in pheromone glands of Helicoverpa armigera: possible up-regulation by Juvenile Hormone

Cited by 38 publications

References 20 publications

Transcriptome analysis of the sex pheromone gland of the noctuid moth Heliothis virescens

Transcriptome analysis of the sex pheromone gland of the noctuid moth Heliothis virescens

Identification of a G protein-coupled receptor for pheromone biosynthesis activating neuropeptide from pheromone glands of the moth Helicoverpa zea

Gene-silencing reveals the functional significance of pheromone biosynthesis activating neuropeptide receptor (PBAN-R) in a male moth

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