The identification of a nuclear factor Akirin with regulating the expression of antimicrobial peptides in red swamp crayfish (Procambarus clarkii)

Xiong, Haoran; Jiang, Yifeng; Ji, Tongwei; Zhang, Yingying; Wei, Wenzhi; Yang, Hui

doi:10.1016/j.ijbiomac.2021.04.153

Cited by 7 publications

(1 citation statement)

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“…In mice, Akirin2 interacts with BAF60a/b/c to respond to immune effectors in macrophages, thereby upregulating interleukin IL-6 expression ( Tartey et al, 2014 ). The impact of Akirin on the immune functions of Litopenaeus vannamei ( Hou et al, 2013 ), Procambarus clarkia ( Xiong et al, 2021 ), Sogatella furcifera ( Chen et al, 2018 ), Ixodes scapularis ( de la Fuente et al, 2013 ), Caenorhabditis elegans ( Bowman et al, 2020 ), and other species has been observed, in addition to developmental abnormalities such as tissue damage, nymphal metamorphosis failure, reduced vector capacity, decreased survival rate, and reduced reproductive fitness ( Almazán et al, 2005 ; de la Fuente et al, 2006 ). Inhibition of Akirin expression using gene knockdown or RNAi techniques affects embryonic development in mice, fruit flies, mosquitoes, ticks, and nematodes ( Goto et al, 2008 ; Qu et al, 2014 ; Bosch et al, 2018 ; 2019 ); muscle development in mice and ducks ( Macqueen et al, 2010 ; Mobley et al, 2014 ; Sun et al, 2016 ); neural development in Xenopus laevis ( Liu et al, 2017 ); and stress responses, feeding, and growth in ticks ( Busby et al, 2012 ; Rahman et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Role of nuclear protein Akirin in the modulation of female reproduction in Nilaparvata lugens (Hemiptera: Delphacidae)

Gou,

Zhang,

Chen

et al. 2024

Front. Physiol.

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Introduction: Akirin as a highly conserved transcription factor, exerts a profound influence on the growth, development, immune response, and reproductive processes in animals. The brown planthopper (BPH), Nilaparvata lugens, a major pest in rice production in Asia, possesses high reproductive capacity, a critical factor contributing to reduced rice yields. The aims of this study were to demonstrate the regulatory role of Akirin in the reproduction of BPH.Methods: In this study, quantitative PCR (qPCR) was used to detect the mRNA expression of genes. RNA interference (RNAi) was used to downregulate the expression of Akirin gene, and RNA sequencing (RNA-seq) was used to screen for differentially expressed genes caused by Akirin downregulation. Hormone contents were measured with the enzyme linked immunosorbent assay (ELISA), and protein content was evaluated with the bicinchoninic acid (BCA) method.Results: Using BPH genome data, we screened for an Akirin gene (NlAkirin). An analysis of tissue-specific expressions showed that NlAkirin was expressed in all tissues tested in female BPH, but its expression level was highest in the ovary. After inhibiting the mRNA expression of NlAkirin in BPH females, the number of eggs laid, hatching rate, and number of ovarioles decreased. Transcriptome sequencing was performed, following a NlAkirin double-stranded RNA treatment. Compared with the genes of the control, which was injected with GFP double-stranded RNA, there were 438 upregulated genes and 1012 downregulated genes; the expression of vitellogenin (Vg) and vitellogenin receptor (VgR) genes as well as the mRNA expression of genes related to the target of rapamycin (TOR), juvenile hormone (JH), and insulin pathways involved in Vg synthesis was significantly downregulated. As a result of NlAkirin knockdown, the titers of JH III and Ecdysone (Ecd) were downregulated in unmated females but returned to normal levels in mated females. The ovarian protein contents in both unmated and mated females were downregulated.Discussion and conclusion: Our results suggest that NlAkirin affects female BPH reproduction by regulating the mRNA expression of genes related to the Vg, VgR, TOR, JH, and insulin signaling pathways, in addition to the titers of JH III and Ecd. The findings of this research provide novel insights into the regulatory role of Akirin in insect reproductive capacity.

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