The Identification of 3:5:3'-L-Triiodothyronine in Human Plasma

Gross, J.; Pitt‐Rivers, Rosalind

doi:10.1016/s0140-6736(52)91952-1

Cited by 279 publications

(63 citation statements)

References 3 publications

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“…The relative specificity of nuclear sites for T3 supports recent metabolic studies which have suggested that the hormonal action of T4 is derived largely, if not exclusively, from its conversion to Ts (4,10,24). The concept that Ts is the "active" hormone was first proposed by Gross and Pitt-Rivers (25) (28) who found significant conversion of T4 to Ta in athyreotic subjects maintained on T8.…”

Section: Discussionsupporting

confidence: 58%

Limited Binding Capacity Sites for l-Triiodothyronine in Rat Liver Nuclei

Oppenheimer¹,

Schwartz²,

Koerner³

et al. 1974

J. Clin. Invest.

167

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A B S T R A C T Further studies have been performed to define the kinetic characteristics of nuclear triiodothyronine (T3) binding sites in rat liver (J. Clin. Endocrinol. Metab. 1972. 35: 330). Sequential determination of labeled T3 associated with nuclei and cytoplasm over a 4-h period allowed analysis of the relationship of T3 in nuclear and cytoplasmic compartments. A rapid interchange of hormone between nuclei and cytoplasm was demonstrated, and in vitro incubation experiments with nuclei yielded no evidence favoring metabolic transformation of T3 by the nuclei. In vivo displacement experiments were performed by subcellular fractionation of liver i h after injection of ["JI]T3 with increasing quantities of unlabeled T3. The nuclear binding capacity for T3 could be defined (0.52 ng/mg DNA). Analysis of these experiments also allowed an estimation of the association constant of nuclear sites for T3 (4.7 X 10"M'). The affinity of these sites for T3 was estimated to be 20-40 fold greater than for thyroxine (T4). Chromatographic analysis of the nuclear radioactivity after injection of labeled T4 indicated that the binding of T4 by the nucleus could not be attributed to in vivo conversion of T4 to T3 but reflected intrinsic cross-reactivity of the two iodothyronines at the nuclear binding sites.

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Section: Discussionsupporting

confidence: 58%

Limited Binding Capacity Sites for l-Triiodothyronine in Rat Liver Nuclei

Oppenheimer¹,

Schwartz²,

Koerner³

et al. 1974

J. Clin. Invest.

167

View full text Add to dashboard Cite

show abstract

“…MASHIAH et al [17] reported good results in the 16 prematures treated by intraamniotic injection of 250 mg of levothyroxine. Triidothyronine (T 3 ) is similar to thyroxine (T 4 ) in its biological action but is more potent on a molar basis [11]. Approximately 20 per cent of T 4 is converted to T 3 [19].…”

Section: ·£3£·mentioning

confidence: 99%

Intraamniotic Triidothyronine instillation for prevention of respiratory distress syndrome in pregnancies complicated by hypertension

Schreyer¹,

Caspi²,

Letko³

et al. 1982

Journal of Perinatal Medicine

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“…The thyroid hormones that have been detected in the plasma of mammals were T 4 (108,109) and T 3 (42). T 4 was detected in higher concentrations in the plasma than T3 and was considered to be the primary thyroid hormone in the blood of mammals.…”

Section: Transport Of Thyroid Hormonesmentioning

confidence: 99%