The identification and functional verification of the cinnamate 4-hydroxylase gene from wax apple fruit and its role in lignin biosynthesis during nitric oxide-delayed postharvest cottony softening

Huang, Lina; Guang-bin, WU; Zhang, Shen; Kuang, Feng-Yuan; Fa-he, Chen

doi:10.1016/j.postharvbio.2019.110964

Cited by 20 publications

(6 citation statements)

References 35 publications

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“…Studies have reported that C4H is one of the major flux-controlling enzymes in plant lignification. The expression and activity of C4H can directly affect lignin production [ 68 , 69 ]. The synthesis process of lignin requires the coordinated participation of multiple enzyme genes and is also regulated by the combination of TFs–gene network [ 70 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and Metabolomic Analysis of the Effects of Exogenous Trehalose on Salt Tolerance in Watermelon (Citrullus lanatus)

Yuan

Sun

et al. 2022

Cells

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Trehalose can effectively protect the biomolecular structure, maintain the balance of cell metabolism, and improve the tolerance to various abiotic stresses in plants. However, the molecular mechanism underlying the improvement in salt tolerance by exogenous trehalose in watermelon (Citrullus lanatus) seedlings is still unclear. To understand these molecular mechanisms, in this study, watermelon seedlings under salt stress were treated with various concentrations of exogenous trehalose. An amount of 20 mM exogenous trehalose significantly improved the physiological status; increased the activities of enzymes such as POD, SOD, and CAT; and increased the K+/Na+ ratio in watermelon seedlings under salt stress. RNA-seq and metabolomic analysis were performed to identify the specifically expressed genes and metabolites after trehalose treatment. Watermelon seedlings were divided into salt stress (CK2), control (CK1) and trehalose treatment (T) groups as per the treatment. Overall, 421 shared differentially expressed genes (DEGs) were identified in the two comparison groups, namely CK2–CK1 and T–CK2. Functional annotation and enrichment analysis revealed that the DEGs were mainly involved in MAPK signaling pathway for plant hormone signal transduction and phenylpropanoid biosynthesis. Furthermore, 129 shared differential expressed metabolites (DEMs) were identified in the two comparison groups using liquid chromatography–mass spectrometry, which were mainly involved in the metabolic pathway and phenylpropanoid biosynthesis. The combined transcriptomic and metabolomic analyses revealed that genes involved in phenylpropanoid biosynthesis, plant hormone signal transduction, and carbohydrate biosynthesis pathways, especially bHLH family transcription factors, played an important role in improving salt tolerance of watermelon seedlings after exogenous trehalose treatment.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic and Metabolomic Analysis of the Effects of Exogenous Trehalose on Salt Tolerance in Watermelon (Citrullus lanatus)

Yuan

Sun

et al. 2022

Cells

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“…Anthocyanin and lignin biosynthesis are metabolically interconnected and diverged in their central metabolite, 4-coumaroyl CoA. C4H is known to play a key role in lignin biosynthesis [ 62 ]. Ring et al [ 63 ] suggested that the downregulation of CHS and concomitant induction of FaPRX27 expression diverted the flux from anthocyanins to lignin.…”

Section: Discussionmentioning

confidence: 99%

Anthocyanin Accumulation and Molecular Analysis of Correlated Genes by Metabolomics and Transcriptomics in Sister Line Apple Cultivars

Shi

Liu

Wei

et al. 2022

Life

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Red coloration in apples, an important quality trait, is primarily attributed to the accumulation of anthocyanins. Centuries of breeding have produced a wide variety of apples with different levels of anthocyanins in response to genetic and environmental stimuli. The Huashuo apple shows a much darker red color than its sister line, Huarui. Thirteen different anthocyanins were detected in Huashuo and Huarui apples, of which ten were significantly more abundant in Huashuo apples, confirming that the color difference is indeed attributed to high anthocyanins accumulation rather than the types of anthocyanins. In particular, the contents of cyanidin 3-O-galactoside levels were highest among anthocyanins in both cultivars, reaching >5000 μg·g−1 at the last color transition stage in Huashuo apples, while only >3000 μg·g−1 in Huarui apples. Moreover, the expression of most structural genes, especially DFR, CHI, and 4CL associated with anthocyanin synthesis, were higher in Huashuo apples than in Huarui apples. Combined transcriptomics, metabolomics, and qRT-PCR analysis revealed that six transcription factors from the MYB and bZIP transcription factor families likely play key roles in the dark coloring of Huashuo apples. These results provide deeper insights into apple coloring and suggest a series of candidate genes for breeding anthocyanin-rich cultivars.

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“…Pristijono et al [ 18 ] investigated changes in apple slice infiltration using an NO donor diethylenetriamine-nitric oxide (DETANO) solution for 60 s and found that NO could effectively inhibit the development of surface browning and extend postharvest life. It was also reported that the softening of harvested wax apple fruit was retarded by NO [ 19 ]. During storage at 18 °C, a higher NO content could further regulate apple fruit ripening [ 20 ].…”

Section: Effects Of No In Postharvest Fruitsmentioning

confidence: 99%

“…NO could delay kiwifruit senescence during storage, in which relative expression of the POD , PAL and CHT genes was up-regulated [ 6 ]. The expression of SsC4H was down-regulated in the NO-retarded cottony softening of harvested wax apple fruits [ 19 ]. NO also up-regulated relative expression of the LeACO1 , LeACOH2 and LeACO4 genes during tomato storage [ 71 ].…”

Section: Modulation Of Gene Expression By No During Fruit Storagementioning

confidence: 99%

Role of Nitric Oxide in Postharvest Senescence of Fruits

Liao

2022

IJMS

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Nitric oxide (NO) acts as a gaseous signalling molecule and is considered to be a key regulator in the postharvest storage of fruits. Postharvest senescence is one of the most serious threats affecting the usage and economic value of fruits. Most recent studies have found that exogenous NO application can effectively improve the quality and prolong the shelf life of fruit postharvest by inhibiting postharvest diseases and alleviating chilling injury. Understanding the roles of NO is essential to elucidating how NO activates the appropriate set of responses to postharvest senescence. Here, we concluded that exogenous NO treatment alleviated senescence in postharvest fruit and attributed this to the following factors: (1) ethylene biosynthesis, (2) the antioxidant system, (3) polyamine metabolism and γ-aminobutyric acid (GABA) shunting, (4) cell wall metabolism, (5) sugar metabolism, (6) energy metabolism, (7) the CRT/DRE-binding factor (CBF) pathway and (8) S-nitrosylation. Moreover, crosstalk between NO and hydrogen sulfide (H2S), hydrogen peroxide (H2O2), oxalic acid (OA), arginine (Arg), GATA or plant hormone abscisic acid (ABA), melatonin (MT), and methyl jasmonate (MeJA), along with the regulation of key genes, were found to be very important in responses to postharvest senescence. In this study, we focus on the recent knowledge concerning the alleviative effect of NO on postharvest senescence, covering ethylene biosynthesis, the antioxidant system and related gene and protein expression.

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The identification and functional verification of the cinnamate 4-hydroxylase gene from wax apple fruit and its role in lignin biosynthesis during nitric oxide-delayed postharvest cottony softening

Cited by 20 publications

References 35 publications

Transcriptomic and Metabolomic Analysis of the Effects of Exogenous Trehalose on Salt Tolerance in Watermelon (Citrullus lanatus)

Transcriptomic and Metabolomic Analysis of the Effects of Exogenous Trehalose on Salt Tolerance in Watermelon (Citrullus lanatus)

Anthocyanin Accumulation and Molecular Analysis of Correlated Genes by Metabolomics and Transcriptomics in Sister Line Apple Cultivars

Role of Nitric Oxide in Postharvest Senescence of Fruits

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