The Iberian endemic species Ranunculus cabrerensis Rothm.: an intricate history in the Ranunculus parnassiifolius L. polyploid complex

Cires, Eduardo; Prieto, José Antonio Fernández

doi:10.1007/s00606-011-0529-9

Cited by 9 publications

(12 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Total genomic DNA from fresh material and silica gel‐dried leaf tissue was collected for molecular analysis. DNA was isolated applying an extraction method based on the CTAB procedure (Doyle & Doyle, ) with slight modifications (see Cires & Fernández Prieto, ) and using a Dneasy Plant Mini Kit (Qiagen, CA, USA). The whole ITS (including ITS1, the 5.8S rDNA, and ITS2) was amplified as a single piece with primers 17SE and 26SE (Sun et al ., ; Hörandl et al ., ).…”

Section: Methodsmentioning

confidence: 99%

“…muniellensis from the R. parnassiifolius sensu lato ( s.l. ) polyploid complex, and that they should consequently be treated as an independent species ( R. cabrerensis vs. R. parnassiifolius ), constituting an evolutionary line in itself (Cires & Fernández Prieto, ). However, taxonomic rearrangements of the group lack a phylogenetic context and a complete biogeographical study.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Unravelling the evolutionary history of the polyploid complexRanunculus parnassiifolius(Ranunculaceae)

Cires

Cuesta

Vargas

et al. 2012

Biol J Linn Soc Lond

Self Cite

View full text Add to dashboard Cite

Ranunculus L. represents the largest genus within Ranunculaceae, comprising more than 600 species with a worldwide distribution. However, there are still many gaps in our knowledge of the infrageneric taxonomy and evolution of Ranunculus. In this regard, intraspecific variation of the polyploid complex Ranunculus parnassiifolius remains under discussion. To reconstruct the biogeographical history of the polyploid complex R. parnassiifolius, 20 populations distributed throughout the Cantabrian Mountains, Pyrenees, and Alps were investigated. Phylogenetic studies were based on nuclear internal transcribed spacers (ITS) and plastid (rpl32‐trnL, rps16‐trnQ) sequence data, analysed using Bayesian approaches as well as the evolution of morphological characters. Additionally, biogeographical patterns were conducted using statistical dispersal–vicariance analysis. The analyses presented here support the recognition of two evolutionary independent units: R. cabrerensis sensu lato (s.l.) and R. parnassiifolius s.l. Furthermore gradual speciation depending on the biogeographical territory is proposed, and optimal reconstructions have probably favoured the ancestor of Ranunculus parnassiifolius as originating in the Iberian Peninsula. © 2012 The Linnean Society of London, Biological Journal of the Linnean Society, 2012, 107, 477–493.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Unravelling the evolutionary history of the polyploid complexRanunculus parnassiifolius(Ranunculaceae)

Cires

Cuesta

Vargas

et al. 2012

Biol J Linn Soc Lond

Self Cite

View full text Add to dashboard Cite

show abstract

“…polyploid complex. However, as a result of recent studies, we have put forward numerous arguments to support the separation of these taxa, and consequently to treat them as independent species (Cires 2011;Cires & Fernández Prieto 2012). In addition to this, within R. cabrerensis it is possible to differentiate two subspecies: R. cabrerensis subsp.…”

Section: Introductionmentioning

confidence: 99%

“…cabrerensis and R. cabrerensis subsp. muniellensis (Bueno, Fern.Casado & Fern.Prieto) Fern.Prieto & Cires (for more details on the subspecies differentiation at morphological and molecular level see Bueno et al (1992); Cires (2011); Cires & Fernández Prieto (2012)). The first grows in small, localised populations on rock-fields and coarse rocky screes (rock-slides) at altitudes of 1750 to 2450 m, Genetic diversity in Ranunculus cabrerensis in the region of Castile and Leon (Northwest Iberian Peninsula).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and structure in fragmented populations of the endangered species Ranunculus cabrerensis (Ranunculaceae): implications for conservation

2012

Self Cite

View full text Add to dashboard Cite

Ranunculus cabrerensis is an endemic and endangered species of the Northwestern Iberian Peninsula. The molecular markers AFLP and ISSR were used to investigate the genetic diversity and population structure of four populations across its known distribution. Fifteen selective primer combinations of AFLP and seventeen ISSR primer combinations produced a total of 2830 and 103 unambiguously repeatable fragments respectively, of which 97.57 and 81.38% were polymorphic for both markers. The genetic diversity of R. cabrerensis at species level was high (HE = 0.294 by ISSR and HE = 0.191 by AFLP) and differentiation between sampled locations was also relatively high (GST = 0.316 and 0.158 by ISSR and AFLP analysis respectively) compared to other studies of endangered and rare species using the same techniques. The analysis of molecular variance (AMOVA) indicated that the main genetic variation was within sampled locations (73% by AFLP; 52% by ISSR), even though the variation among locations was also significant. Principal Coordinates, NeighborNet and Bayesian analyses revealed a weak but significant relationship between the genetic structures of different populations in R. cabrerensis, with gene flow acting as a homogenizing force that prevents stronger differentiation of populations. Finally, suggestions for conservation strategies to preserve the genetic resources of this species are outlined.

show abstract

“…cabrerensis Rothm.) from the Cantabrian Mountains (Laínz 1964;Cires & Fernández Prieto 2012). The occurrences of hybridization among those species of Ranunculus have been suggested on the basis on morphological intermediacy of some individuals found in the same habitat as their putative progenitors.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of two natural hybrids from the Iberian Peninsula: Ranunculus × luizetii and R. × peredae (Ranunculaceae)

2012

Self Cite

View full text Add to dashboard Cite

Two nothospecies, Ranunculus ×luizetii and R. ×peredae (Ranunculaceae), were analyzed and discussed. For this purpose, Amplified Fragment Length Polymorphism (AFLP) markers, nuclear rDNA sequences (ITS1, 5.8S and ITS2) and pollen viability were conducted. The profiles of these hybrid samples were compared to their putative progenitors. Several additive polymorphic sites detected in the ITS sequences of the hybrid samples (R. ×luizetii and R. ×peredae) also confirmed their derived origins from ribotypes of their parental taxa (R. parnassiifolius subsp. parnassiifolius × R. pyrenaeus; R. amplexicaulis × R. cabrerensis subsp. cabrerensis, respectively). Despite the lack of exclusive AFLP markers reported in both hybrids, presumably due to effects of introgression, the concerted evolution of many rDNA polymorphisms towards either of the parental ribotypes indicated their ancient origin. Pollen fertility estimation in R. ×luizetii presented a mean value of 60.58%, which showed that hybrid samples are well established and fertile. However, a larger difference was observed in R. ×peredae, where the mean value of pollen fertility was very low (18.91%).

show abstract

The Iberian endemic species Ranunculus cabrerensis Rothm.: an intricate history in the Ranunculus parnassiifolius L. polyploid complex

Cited by 9 publications

References 57 publications

Unravelling the evolutionary history of the polyploid complexRanunculus parnassiifolius(Ranunculaceae)

Unravelling the evolutionary history of the polyploid complexRanunculus parnassiifolius(Ranunculaceae)

Genetic diversity and structure in fragmented populations of the endangered species Ranunculus cabrerensis (Ranunculaceae): implications for conservation

Molecular characterization of two natural hybrids from the Iberian Peninsula: Ranunculus × luizetii and R. × peredae (Ranunculaceae)

Contact Info

Product

Resources

About