The IAPP Toxicity on Rats, Raccoons and Degus in HeLa Cells

Abstract

Type 2 diabetes is the most common form of diabetes, where the body suffers from insulin resistance. The islet amyloid polypeptide (IAPP) is a secretory product of beta cells in pancreatic islets of Langerhans that also inhibits glucagon and insulin secretion. The aggregation of IAPP is present in pancreatic islet amyloid deposits seen especially in Type 2 diabetes, in humans and other mammalian species. The objective of this study was to determine the effects of the animal toxicity on mammalian cells. In this work, the human IAPP sequence and the effect on mammalian cells was compared to the IAPP from degu, rat, and raccoon. Molecular techniques such as MTT and LDH Cytotoxicity assay were used to test the effect of human and animal IAPP on HeLa cells. The MTT results show a consistent drop in cell viability as the concentration of IAPP from a human, degu, rat and raccoon increases. LDH cytotoxicity shows the highest toxicity in human IAPP compared to the rest of the studied animals. Although when mixing both the human and animal IAPPs, the toxicity of the human IAPP decreased whereas the viability had no significant effect. Further molecular analysis is necessary to determine the correlation between animal IAPP toxicity and the incidence of Type 2 diabetes.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.