The <i>white</i> gene of the tephritid fruit fly <i>Bactrocera tryoni</i> is characterized by a long untranslated 5′ leader and a 12 kb first intron

Bennett, Craig L.; Frommer, Marianne

doi:10.1046/j.1365-2583.1997.00188.x

Cited by 25 publications

(24 citation statements)

References 19 publications

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“…a and b are fragments released by EcoRI; c-e are fragments released by HindIII; f and g are fragments released by PstI digestion canonical polyadenylation signal (AATAAT). A similar non-canonical signal is found in the white genes of Anopheles gambiae and Bactrocera tryoni Bennett and Frommer 1997). Sequence alignment of the Bmwh3 PCR fragment (nt 810±1219) with the homologous region of the Bmwh3 cDNA reveals nine nucleotide substitutions, one of which (at nt 927) results in the substitution of a proline for a serine (Fig.…”

Section: Isolation Of Abc Transporter Genessupporting

confidence: 57%

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Identification and characterisation of a silkworm ABC transporter gene homologous to Drosophila white

Abraham¹,

Sezutsu²,

Kanda³

et al. 2000

Mol Gen Genet

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In the silkworm, Bombyx mori, many eye- and egg-colour mutations affecting the synthesis and accumulation of ommochrome pigments have been described. In order to understand the pigment precursor transporters involved, ABC transporter genes homologous to the Drosophila white gene were isolated from the silkworm. Reverse transcriptase-polymerase chain reactions (RT-PCR) using embryonic mRNA amplified three cDNA fragments, named Bmwh1, Bmwh2 and Bmwh3 that showed homology to the white gene. Since Bmwh3 shows the highest degree of sequence identity and a similar expression pattern to the Drosophila homologue, we characterised this gene further. A 2667-bp Bmwh3 cDNA isolated from an embryonic library has one ORF encoding a polypeptide of 687 amino acids. The predicted protein has one ATP-binding domain, six transmembrane-spanning segments and high similarity to the Drosophila WHITE protein. Southern analysis indicates that Bmwh3 is a single-copy gene. Polyadenylated Bmwh3 transcripts about 2.7 kb long were detected in eggs, Malpighian tubules and pupal heads, but not in testes, posterior silk glands or fat body cells. The level of Bmwh3 mRNA was reduced in w3 and w3ol mutants but normal in other egg- and eye-colour mutants, suggesting that Bmwh3 correspond to the w3 locus. Genetic analysis was used to map the cloned gene to chromosome 10.

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Section: Isolation Of Abc Transporter Genessupporting

confidence: 57%

“…The ATP binding domain is encoded by ®ve exons, while the homologous region in dipteran white genes is coded by two exons (Fig. 4C) O'Hare et al 1984;Garcia et al 1996;Bennett and Frommer 1997). At least two intron (O'Hare et al 1984;Besansky et al 1995).…”

Section: Discussionmentioning

confidence: 99%

Identification and characterisation of a silkworm ABC transporter gene homologous to Drosophila white

Abraham¹,

Sezutsu²,

Kanda³

et al. 2000

Mol Gen Genet

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“…Placement of the wingless intron for all the diopsids corresponds to the intron between the fourth and fth exon of Drosophila melanogaster (Rijsewijk et al, 1987), which is »100-180 bp long. Placement of the white intron in diopsids corresponds to the intron between exons 3 and 4a in Bactrocera tryoni (Bennett and Frommer, 1997), an intron that is »50-90 bp long. Because the extreme sequence divergence in these introns provided little alignment stability, they were therefore excluded from all subsequent analyses.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic Utility of Different Types of Molecular Data Used to Infer Evolutionary Relationships among Stalk-Eyed Flies (Diopsidae)

Baker¹,

Wilkinson²,

DeSalle³

2001

Systematic Biology

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Abstract.-A phylogenetic hypothesis of relationships among 33 species of stalk-eyed ies was generated from a molecular data set comprising three mitochondrial and three nuclear gene regions. A combined analysis of all the data equally weighted produced a single most-parsimonious cladogram with relatively strong support at the majority of nodes. The phylogenetic utility of different classes of molecular data was also examined. In particular, using a number of different measures of utility in both a combined and separate analysis framework, we focused on the distinction between mitochondrial and nuclear genes and between faster-evolving characters and slower-evolving characters. For the rst comparison, by nearly any measure of utility, the nuclear genes are substantially more informative for resolving diopsid relationships than are the mitochondrial genes. The nuclear genes exhibit less homoplasy, are less incongruent with one another and with the combined data, and contribute more support to the combined analysis topology than do the mitochondrial genes. Results from the second comparison, however, provide little evidence of a clear difference in utility. Despite indications of rapid divergence and saturation, faster-evolving characters in both the nuclear and mitochondrial data sets still provide substantial phylogenetic signal. In general, inclusion of the more rapidly evolving data consistently improves the congruence among partitions. [Diopsidae; incongruence; nuclear genes; partitioned Bremer support; saturation.] Flies in the family Diopsidae are characterized by the elongation of the head into long stalks, with the eyes and antennae laterally displaced at the ends of these stalks. In several species, this elongation is so extreme that the length of their eye stalks exceeds the length of their body. Several dipteran families possess hypercephalic species (Grimaldi and Fenster, 1989;Wilkinson and Dodson, 1997), but the Diopsidae are unique in that both males and females of all the species within the family have some degree of head modi cation. Recently, experiments examining the importance of eye stalks in the mating system of diopsids have provided considerable information about their adaptive signicance (Burkhardt and de la Motte, 1985Motte, , 1988Lorch et al., 1993;Wilkinson, 1993;Burkhardt et al., 1994;Wilkinson and Reillo, 1994;Wilkinson and Dodson, 1997). For the majority of diopsid species, males have markedly larger eye stalks than females, and several studies have demonstrated that this increased eye span functions as an ornament for both male combat (Burkhardt and de la Motte, 1983Motte, , 1987Lorch et al., 1993) and 4 Present address (and address for correspondence):

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“…The three largest autosomes are metacentric or submetacentric and easily identified by their chromosome length, arm raProbe Description white 5.1-kb XhoI fragment, containing exons 2-6 of the B. tryoni white eye-colour gene; cloned into pBluescript KS+ phagemid (Bennett and Frommer 1997) scarlet cDNA fragment, generated by the 3′ RACE (rapid amplification of cDNA ends) protocol (Frohman et al 1988), containing part of exon 4 through to exon 7 of the B. tryoni scarlet eye-colour gene; homologous to scarlet in D. melanogaster (Tearle et al 1989) and topaz in Lucilia cuprina (Elizur et al 1990); cloned into pBluescript KS+ phagemid (C.L. Bennett and M. Frommer, unpublished data) hsp70 Ceratitis capitata heat shock gene coding for the heat shock 70 kDa protein; 2.9-kb genomic DNA cloned into pGem72(+) vector (Papadimitriou et al 1998) sod1 Ceratitis capitata superoxide dismutase 1 gene; 4-kb genomic DNA cloned into pBluescript SK+ vector (Banks et al 1995) ps2α Ceratitis capitata homologue of the inflated ps2α integrin gene of D. melanogaster tio, and the presence of secondary constrictions.…”

Section: Mitotic Chromosomesmentioning

confidence: 99%

“…The white gene of B. tryoni has been shown to be homologous to the white gene of C. capitata, L. cuprina, and D. melanogaster by DNA sequence comparison (Bennett and Frommer 1997). In the B. tryoni polytene chromosome complement, the white gene of B. tryoni and the ps2α gene of C. capitata are clustered on chromosome arm 5L: white maps to 71B and ps2α maps to 68C.…”

Section: Chromosomementioning

confidence: 99%

Mitotic and polytene chromosome analyses in the Queensland fruit fly,Bactrocera tryoni(Diptera: Tephritidae)

Zhao¹,

Frommer²,

Sved³

et al. 1998

Genome

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The Queensland fruit fly, Bactrocera tryoni, like the Mediterranean fruit fly, Ceratitis capitata, has a diploid complement of 12 chromosomes, including five pairs of autosomes and a XX/XY sex chromosome pair. Characteristic features of each chromosome are described. Chromosomal homology between B. tryoni and C. capitata has been determined by comparing chromosome banding pattern and in situ hybridisation of cloned genes to polytene chromosomes. Although the evidence indicates that a number of chromosomal inversions have occurred since the separation of the two species, synteny of the chromosomes appears to have been maintained.

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The white gene of the tephritid fruit fly Bactrocera tryoni is characterized by a long untranslated 5′ leader and a 12 kb first intron

Cited by 25 publications

References 19 publications

Identification and characterisation of a silkworm ABC transporter gene homologous to Drosophila white

Identification and characterisation of a silkworm ABC transporter gene homologous to Drosophila white

Phylogenetic Utility of Different Types of Molecular Data Used to Infer Evolutionary Relationships among Stalk-Eyed Flies (Diopsidae)

Mitotic and polytene chromosome analyses in the Queensland fruit fly,Bactrocera tryoni(Diptera: Tephritidae)

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