THE <i>tolC</i> LOCUS IN <i>ESCHERICHIA COLI</i> K12

Whitney, Eleanor

doi:10.1093/genetics/67.1.39

Cited by 90 publications

(3 citation statements)

References 17 publications

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“…The mutations appear to cause nonspecific increases in permeability (18,20). Various colicin-tolerant or -resistant mutants with altered membranes are also hypersensitive to certain dyes, drugs, and detergents (10,16,25). One of the colicin-tolerant mutants, studied in detail, grew poorly above 40 C; 0.5 M sucrose gave slight protection.…”

Section: Discussionmentioning

confidence: 99%

Osmotic Reversal of Temperature Sensitivity in Escherichia coli

Bilsky

Armstrong

1973

J Bacteriol

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Forty temperature-sensitive mutants, unable to grow on tryptone or nutrient agar at 42 C, were isolated from Escherichia coli K-12. When 0.5% NaCl was added to the medium, 32 grew at the nonpermissive temperature. Several were tested with different amounts of NaCl added to tryptone broth; all grew best when the osmolality of the medium was between 400 and 1,000 milliosmolal. One of the mutants was studied in more detail. Sucrose, inositol, KCl, and MgCl 2 , as well as NaCl, permitted growth at 42 C. Glycerol, however, had no effect. When shifted from 30 to 42 C without osmotic protection, the mutant stopped growing but did not lyse, die, or leak significant amounts of intracellular material. In a similar shift experiment, a second mutant leaked all of its trichloroacetic acid-soluble pools into the medium. The majority of the mutants were hypersensitive to certain antibiotics, indicating possible cell envelope defects.

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Section: Discussionmentioning

confidence: 99%

Osmotic Reversal of Temperature Sensitivity in Escherichia coli

Bilsky

Armstrong

1973

J Bacteriol

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“…C-2301 was isolated by spotting samples of a chloroform-treated overnight culture of RC903, a colicin El-carrying Salmonella typhimurium, on a lawn of C-2128 supplemented with streptomycin. Colonies which grew were tested for their sensitivity to deoxycholate (36). One of the colonies, C-2301, was shown to be tolCby its insensitivity to colicin El produced by RC903 and by its sensitivity to concentrations of deoxycholate, which did not affect the growth of the parent strain C-2128.…”

Section: Methodsmentioning

confidence: 99%

Escherichia coli deoxyribonucleic acid synthesis mutants: their effect upon bacteriophage P2 and satellite bacteriophage P4 deoxyribonucleic acid synthesis

Bowden¹,

Twersky²,

Calendar³

1975

J Bacteriol

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Escherichia coli C strains containing different deoxyribonucleic acid (DNA) synthesis mutations have been tested for their support of the DNA synthesis of bacteriophage P2 and its satellite phage P4. Bacteriophage P2 requires functional dnaB, dnaE, and dnaG E. coli gene products for DNA synthesis, whereas it does not require the products of the dnaA, dnaC, or dnaH genes. In contrast, the satellite virus P4 requires functional dnaE and dnaH gene products for DNA synthesis and does not need the products of the dnaA, dnaB, dnaC, and dnaG genes. Thus the P2 and P4 genomes are replicated differently, even though they are packaged in heads made of the same protein.

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“…(ii) Cotransduction studies of exuR. The exuR locus was more precisely mapped by de-termining cotransduction frequencies of different alleles of this gene (exuRI, exuRIO, exuR-2, exuR10-16) with toiC (33), uxaAl (20), uxaCI (19), exuT (18), and argG (4), which were previously localized between min 65 and 68 (Table 6).…”

Section: Resultsmentioning

confidence: 99%

Regulation of Escherichia coli K-12 Hexuronate System Genes: exu Regulon

1980

J Bacteriol

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THE tolC LOCUS IN ESCHERICHIA COLI K12

Cited by 90 publications

References 17 publications

Osmotic Reversal of Temperature Sensitivity in Escherichia coli

Osmotic Reversal of Temperature Sensitivity in Escherichia coli

Escherichia coli deoxyribonucleic acid synthesis mutants: their effect upon bacteriophage P2 and satellite bacteriophage P4 deoxyribonucleic acid synthesis

Regulation of Escherichia coli K-12 Hexuronate System Genes: exu Regulon

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