The
            <i>Synechococcus</i>
            Strain PCC 7942
            <i>glnN</i>
            Product (Glutamine Synthetase III) Helps Recovery from Prolonged Nitrogen Chlorosis

Sauer, Jörg; Dirmeier, Ulrike; Forchhammer, Karl

doi:10.1128/jb.182.19.5615-5619.2000

Cited by 42 publications

(49 citation statements)

References 19 publications

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“…Synthesis of GlnN following nitrogen deprivation is impaired not only in an NtcA-deficient mutant but also in a mutant deficient in P II (Sauer et al, 2000), suggesting a link between P II and NtcA under conditions of nitrogen deprivation. To investigate this potential relationship, protein synthesis patterns of Synechococcus wild-type, P II -and NtcA-deficient mutants were compared (Aldehni et al, 2003).…”

Section: Acclimation To Nitrogen Deprivationmentioning

confidence: 95%

“…One of the genes exhibiting a putative non-canonical NtcA site is glnN, encoding an alternative glutamine synthetase of type III (Reyes et al, 1997). The glnN product helps Synechococcus cells recover more rapidly after a prolonged period of nitrogen chlorosis, although this gene product is not essential for survival (Sauer et al, 2000). Differential affinity of NtcA towards various DNA-binding sites provides a simple mechanism to modulate gene expression in response to a gradual limitation of the nitrogen source.…”

Section: Acclimation To Nitrogen Deprivationmentioning

confidence: 99%

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Acclimation of unicellular cyanobacteria to macronutrient deficiency: emergence of a complex network of cellular responses

2005

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Cyanobacteria are equipped with numerous mechanisms that allow them to survive under conditions of nutrient starvation, some of which are unique to these organisms. This review surveys the molecular mechanisms underlying acclimation responses to nitrogen and phosphorus deprivation, with an emphasis on non-diazotrophic freshwater cyanobacteria. As documented for other micro-organisms, nutrient limitation of cyanobacteria elicits both general and specific responses. The general responses occur under any starvation condition and are the result of the stresses imposed by arrested anabolism. In contrast, the specific responses are acclimation processes that occur as a result of limitation for a particular nutrient; they lead to modification of metabolic and physiological routes to compensate for the restriction. First, the general acclimation processes are discussed, with an emphasis on modifications of the photosynthetic apparatus. The molecular mechanisms underlying specific responses to phosphorus and nitrogen-limitation are then outlined, and finally the cross-talk between pathways modulating specific and general responses is described.

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Section: Acclimation To Nitrogen Deprivationmentioning

confidence: 95%

Section: Acclimation To Nitrogen Deprivationmentioning

confidence: 99%

Acclimation of unicellular cyanobacteria to macronutrient deficiency: emergence of a complex network of cellular responses

2005

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“…In addition to this orthodox promoter structure, which matches that of the Escherichia coli class II Crp-dependent promoters, NtcA can activate from positions further upstream or from sequences not matching the reported consensus (21). NtcA binding motifs were found in a few genes or operons experimentally characterized in S. elongatus (22)(23)(24) and in others predicted to be controlled by NtcA in the cyanobacterium S. elongatus PCC 6301 (25), which is almost identical to S. elongatus. Remarkably, NtcA binding motifs were found in association with 29 of the 30 transcription units in class 4 ( Fig.…”

Section: Y32amentioning

confidence: 99%

PipX, the coactivator of NtcA, is a global regulator in cyanobacteria

Espinosa¹,

Rodríguez

Salinas³

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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To modulate the expression of genes involved in nitrogen assimilation, the cyanobacterial P II -interacting protein X (PipX) interacts with the global transcriptional regulator NtcA and the signal transduction protein P II , a protein found in all three domains of life as an integrator of signals of the nitrogen and carbon balance. PipX can form alternate complexes with NtcA and P II , and these interactions are stimulated and inhibited, respectively, by 2-oxoglutarate, providing a mechanistic link between P II signaling and NtcA-regulated gene expression. Here, we demonstrate that PipX is involved in a much wider interaction network. The effect of pipX alleles on transcript levels was studied by RNA sequencing of S. elongatus strains grown in the presence of either nitrate or ammonium, followed by multivariate analyses of relevant mutant/control comparisons. As a result of this process, 222 genes were classified into six coherent groups of differentially regulated genes, two of which, containing either NtcA-activated or NtcA-repressed genes, provided further insights into the function of NtcA-PipX complexes. The remaining four groups suggest the involvement of PipX in at least three NtcA-independent regulatory pathways. Our results pave the way to uncover new regulatory interactions and mechanisms in the control of gene expression in cyanobacteria.nitrogen regulation | transcription | translation | photosynthesis

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“…Interestingly, GSIII is also induced in the cyanobacterium Synechococcus during the early stages of nitrogen deprivation (Sauer et al, 2000). In addition, Mock et al (2008) measured increased transcript levels of two NAD(P)H-GOGAT genes (ProtIDs 29861 and 269160) along with a ferredoxin-dependent form of this enzyme (ProtID 269900).…”

Section: Nitrogen Assimilationmentioning

confidence: 99%

The Response of Diatom Central Carbon Metabolism to Nitrogen Starvation Is Different from That of Green Algae and Higher Plants

Hockin

Möck²,

Mulholland³

et al. 2011

Plant Physiology

321

304

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The availability of nitrogen varies greatly in the ocean and limits primary productivity over large areas. Diatoms, a group of phytoplankton that are responsible for about 20% of global carbon fixation, respond rapidly to influxes of nitrate and are highly successful in upwelling regions. Although recent diatom genome projects have highlighted clues to the success of this group, very little is known about their adaptive response to changing environmental conditions. Here, we compare the proteome of the marine diatom Thalassiosira pseudonana (CCMP 1335) at the onset of nitrogen starvation with that of nitrogen-replete cells using two-dimensional gel electrophoresis. In total, 3,310 protein spots were distinguishable, and we identified 42 proteins increasing and 23 decreasing in abundance (greater than 1.5-fold change; P , 0.005). Proteins involved in the metabolism of nitrogen, amino acids, proteins, and carbohydrates, photosynthesis, and chlorophyll biosynthesis were represented. Comparison of our proteomics data with the transcriptome response of this species under similar growth conditions showed good correlation and provided insight into different levels of response. The T. pseudonana response to nitrogen starvation was also compared with that of the higher plant Arabidopsis (Arabidopsis thaliana), the green alga Chlamydomonas reinhardtii, and the cyanobacterium Prochlorococcus marinus. We have found that the response of diatom carbon metabolism to nitrogen starvation is different from that of other photosynthetic eukaryotes and bears closer resemblance to the response of cyanobacteria.

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The Synechococcus Strain PCC 7942 glnN Product (Glutamine Synthetase III) Helps Recovery from Prolonged Nitrogen Chlorosis

Cited by 42 publications

References 19 publications

Acclimation of unicellular cyanobacteria to macronutrient deficiency: emergence of a complex network of cellular responses

Acclimation of unicellular cyanobacteria to macronutrient deficiency: emergence of a complex network of cellular responses

PipX, the coactivator of NtcA, is a global regulator in cyanobacteria

The Response of Diatom Central Carbon Metabolism to Nitrogen Starvation Is Different from That of Green Algae and Higher Plants

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