The<i>Pseudomonas aeruginosa</i>accessory genome elements influence virulence towards<i>Caenorhabditis elegans</i>

Vasquez-Rifo, Alejandro; Veksler-Lublinsky, Isana; Cheng, Zheyu; Ausubel, F M; Ambros, Victor R.

doi:10.1101/621433

Cited by 3 publications

(5 citation statements)

References 59 publications

(56 reference statements)

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“…Moreover, we implicate R-body production in PA14-mediated ribosomal injury, a virulence mechanism that yields translational inhibition in the nematode host Caenorhabditis elegans 16 . Taken together, these observations identify the R-body as a PA14 virulence factor and raise the possibility that R-body production is a component of the marked strain-dependent variation in pathogenicity observed for P. aeruginosa 13,17,18 .…”

Section: Introductionmentioning

confidence: 72%

“…Expression of the putative rebP1P2 operon is regulated by the transcription factor RcgA and sigma factor FecI2. RcgA and FecI2 had previously been associated with virulence 12,17 , but their exact roles have eluded definition. We have also provided evidence that rebP1P2 expression is stochastic and that it can be observed both in independent biofilms and during association with hosts.…”

Section: Discussionmentioning

confidence: 99%

“…The reb cluster genes rcgA, fecI2, and rapA had previously been shown to contribute to PA14 virulence in C. elegans 12,13,18 . rcgA, specifically, had also been implicated in the ribosomal cleavage that occurs during C. elegans infection 16 .…”

Section: Discussionmentioning

confidence: 99%

“…A substantial fraction (147/241) of P. aeruginosa complete strain genomes, including that of strain UCBPP-PA14 ("PA14"), contain reb gene homologs as part of a cluster that includes the gene for the sigma factor FecI2 and targets of FecI2 10 ; we refer to this region as the "reb cluster". Genes in the reb cluster have been identified as contributing to increased virulence when distinct P. aeruginosa isolates are compared 11,12 . Notably, the popular model strain PAO1 does not contain a reb cluster and this is consistent with its relatively low virulence in a Caenorhabditis elegans infection assay.…”

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confidence: 99%

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Pseudomonas aeruginosa PA14 produces R-bodies, extendable protein polymers with roles in host colonization and virulence

Wang

Lin

Vasquez-Rifo

et al. 2020

Preprint

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reb genes code for R-bodies: large, extendable polymers that are known for their roles in obligate endosymbioses. In the non-endosymbiotic pathogen Pseudomonas aeruginosa, reb homologues are part of a cluster found in virulent strains. Here, we demonstrate that R-bodies are produced in abundance by P. aeruginosa PA14 subpopulations during biofilm growth, identify regulators of reb gene expression, and show that reb genes are required for full colonization and virulence in host models.

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Section: Introductionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Pseudomonas aeruginosa PA14 produces R-bodies, extendable protein polymers with roles in host colonization and virulence

Wang

Lin

Vasquez-Rifo

et al. 2020

Preprint

Self Cite

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“…Interestingly, where it has been measured, pathogens exhibit roughly similar patterns of virulence in these organisms. For example, Pseudomonas species are more virulent than Enterococcus or Providencia, whereas Enterococcus and Providencia are roughly equivalent (SIM AND HIBBERD 2016; MARTIN et al 2017;ARCHER AND PHILLIPS 2018;TROHA et al 2018;VASQUEZ-RIFO et al 2019), B.D. Ackley, Personal Observation).…”

Section: Introductionmentioning

confidence: 99%

A comparative analysis ofCaenorhabditisandDrosophilatranscriptional changes in response to pathogen infection

Unckless

Lansdon

Ackley

2020

Preprint

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We analyzed transcriptomic data from C. elegans and D. melanogaster to compare the expression of orthologous pairs of genes in response to bacterial pathogens. Our results indicated that only a handful of genes that are orthologous between species are differentially expressed in response to pathogens, but that the pattern of expression was different when comparing one-to-one orthologs versus those that are restricted to one of the two organisms.These results suggest that, although broad patterns of susceptibility to bacterial pathogens are conserved, the regulatory framework by which the organisms fight pathogens is less well conserved. Further our results suggest a more complete analysis of the evolutionary changes in organismal responses to pathogens is required.Immune divergence in invertebrates 3 | P a g e Abstract Drosophila melanogaster and Caenorhabditis elegans are well-used invertebrate models for studying the innate immune system. The organisms are susceptible to bacterial pathogens that include Pseudomonas species, (entomophilia -Drosophila) or (aeruginosa -Caenorhabditis), E. faecalis and P. rettgeri, which are or are related to human pathogens. Further, the consequences of exposure to these pathogens, in terms of organismal survival, are roughly equivalent when compared. That is, worms and flies are more susceptible to infection by Pseudomonas than E. faecalis, whereas organismal survival on E. faecalis and P. rettgeri are roughly the same in both. To better understand how these organisms are coordinating their responses to these bacterial pathogens we examined transcriptomes in infected animals. We grouped our analysis based on protein orthology. Of the 3611 pairs analyzed, we found genes whose responses were conserved across the different species at a higher than expected rate for two of the three pathogens. Interestingly within the animals, genes with 1:1 orthologs between species behaved differently. Such genes were more likely to be expressed in D. melanogaster, and less likely to be expressed in C. elegans. From this analysis we found that the gene nucleobindin (nucb-1/NUCB1 in C. elegans and D. melanogaster, respectively) was upregulated in both species in response to Gram negative bacteria. We used RNAi to knock down nucb-1 and found the treated animals were more susceptible to infection by the Gram negative pathogen P. rettgeri than controls. These results provide insight into some of the conserved mechanisms of pathogen defense, but also suggest that these divergent organisms have evolved specific means to orchestrate the defense against pathogens.Immune divergence in invertebrates 4 | P a g e

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An Efficient, Counter-Selection-Based Method for Prophage Curing in Pseudomonas aeruginosa Strains

Shmidov

Zander

Lebenthal-Loinger

et al. 2021

Viruses

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Prophages are bacteriophages in the lysogenic state, where the viral genome is inserted within the bacterial chromosome. They contribute to strain genetic variability and can influence bacterial phenotypes. Prophages are highly abundant among the strains of the opportunistic pathogen Pseudomonas aeruginosa and were shown to confer specific traits that can promote strain pathogenicity. The main difficulty of studying those regions is the lack of a simple prophage-curing method for P. aeruginosa strains. In this study, we developed a novel, targeted-curing approach for prophages in P. aeruginosa. In the first step, we tagged the prophage for curing with an ampicillin resistance cassette (ampR) and further used this strain for the sacB counter-selection marker's temporal insertion into the prophage region. The sucrose counter-selection resulted in different variants when the prophage-cured mutant is the sole variant that lost the ampR cassette. Next, we validated the targeted-curing with local PCR amplification and Whole Genome Sequencing. The application of the strategy resulted in high efficiency both for curing the Pf4 prophage of the laboratory wild-type (WT) strain PAO1 and for PR2 prophage from the clinical, hard to genetically manipulate, 39016 strain. We believe this method can support the research and growing interest in prophage biology in P. aeruginosa as well as additional Gram-negative bacteria.

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ThePseudomonas aeruginosaaccessory genome elements influence virulence towardsCaenorhabditis elegans

Cited by 3 publications

References 59 publications

Pseudomonas aeruginosa PA14 produces R-bodies, extendable protein polymers with roles in host colonization and virulence

Pseudomonas aeruginosa PA14 produces R-bodies, extendable protein polymers with roles in host colonization and virulence

A comparative analysis ofCaenorhabditisandDrosophilatranscriptional changes in response to pathogen infection

An Efficient, Counter-Selection-Based Method for Prophage Curing in Pseudomonas aeruginosa Strains

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