The<i>Penaincisalia amatista</i>species-group (Lepidoptera: Lycaenidae, Eumaeini) in Colombia, insights from<i>mt</i>DNA barcodes and the description of a new species

Prieto, Carlos; Grishin, Nick V.; Hausmann, Axel; Lorenc-Brudecka, Jadwiga

doi:10.1080/14772000.2015.1112314

Cited by 7 publications

(6 citation statements)

References 21 publications

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“…The general goal is to quantify the potential usefulness of reference libraries of DNA barcodes for identification and for resolution of taxonomic problems in this group. In previous studies ( e.g ., Prieto et al, 2016 ; Prieto, Nuñez & Hausmann, 2018 ; Faynel, 2019 ) we found that congruence between DNA and morphology varies among genera. We hypothesized that the incidence of congruence among strictly high Andean genera was lower than among lowland genera.…”

Section: Introductionmentioning

confidence: 62%

“…The checklist was updated using subsequent publications ( e.g . Balint & Faynel, 2008 ; Prieto et al, 2008 ; Duarte & Robbins, 2010 ; Faynel et al, 2011 ; Prieto, 2011 ; Faynel, Busby & Robbins, 2012 ; Robbins, Heredia & Busby, 2015 ; Prieto et al, 2016 ; Prieto & Vargas, 2016 ; Busby et al, 2017 ). When necessary, identifications were verified through genitalic examination.…”

Section: Methodsmentioning

confidence: 99%

“…In contrast with other, better known families, lycaenids lack sufficiently illustrated identification keys, monographs, field guides, or checklists covering regions or countries in a comprehensive and updated manner. The use of DNA barcode sequences and BINs in this group has been limited, but congruence between morphology and barcode sequences is variable ( Prieto, Micó & Galante, 2011 ; Faynel et al, 2011 ; Faynel, Busby & Robbins, 2012 ; Prieto et al, 2016 ; Cong et al, 2016 , 2017 ; Prieto & Lorenc-Brudecka, 2017 ; Busby et al, 2017 ; Prieto, Nuñez & Hausmann, 2018 ; Faynel, 2019 ). In particular, in previous studies it appeared that strictly high Andean genera were more likely, on average, to show incongruence.…”

Section: Introductionmentioning

confidence: 99%

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Congruence between morphology-based species and Barcode Index Numbers (BINs) in Neotropical Eumaeini (Lycaenidae)

Prieto

Faynel²,

Robbins

et al. 2021

PeerJ

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Background With about 1,000 species in the Neotropics, the Eumaeini (Theclinae) are one of the most diverse butterfly tribes. Correct morphology-based identifications are challenging in many genera due to relatively little interspecific differences in wing patterns. Geographic infraspecific variation is sometimes more substantial than variation between species. In this paper we present a large DNA barcode dataset of South American Lycaenidae. We analyze how well DNA barcode BINs match morphologically delimited species. Methods We compare morphology-based species identifications with the clustering of molecular operational taxonomic units (MOTUs) delimitated by the RESL algorithm in BOLD, which assigns Barcode Index Numbers (BINs). We examine intra- and interspecific divergences for genera represented by at least four morphospecies. We discuss the existence of local barcode gaps in a genus by genus analysis. We also note differences in the percentage of species with barcode gaps in groups of lowland and high mountain genera. Results We identified 2,213 specimens and obtained 1,839 sequences of 512 species in 90 genera. Overall, the mean intraspecific divergence value of CO1 sequences was 1.20%, while the mean interspecific divergence between nearest congeneric neighbors was 4.89%, demonstrating the presence of a barcode gap. However, the gap seemed to disappear from the entire set when comparing the maximum intraspecific distance (8.40%) with the minimum interspecific distance (0.40%). Clear barcode gaps are present in many genera but absent in others. From the set of specimens that yielded COI fragment lengths of at least 650 bp, 75% of the a priori morphology-based identifications were unambiguously assigned to a single Barcode Index Number (BIN). However, after a taxonomic a posteriori review, the percentage of matched identifications rose to 85%. BIN splitting was observed for 17% of the species and BIN sharing for 9%. We found that genera that contain primarily lowland species show higher percentages of local barcode gaps and congruence between BINs and morphology than genera that contain exclusively high montane species. The divergence values to the nearest neighbors were significantly lower in high Andean species while the intra-specific divergence values were significantly lower in the lowland species. These results raise questions regarding the causes of observed low inter and high intraspecific genetic variation. We discuss incomplete lineage sorting and hybridization as most likely causes of this phenomenon, as the montane species concerned are relatively young and hybridization is probable. The release of our data set represents an essential baseline for a reference library for biological assessment studies of butterflies in mega diverse countries using modern high-throughput technologies an highlights the necessity of taxonomic revisions for various genera combining both molecular and morphological data.

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Section: Introductionmentioning

confidence: 62%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Congruence between morphology-based species and Barcode Index Numbers (BINs) in Neotropical Eumaeini (Lycaenidae)

Prieto

Faynel²,

Robbins

et al. 2021

PeerJ

Self Cite

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“…Prieto demonstrated for several Penaincisalia species groups that, although the male genitalia differences were quantitative, they were sufficient for species recognition (Prieto et al 2008(Prieto et al , 2016. This statement stands also for Penaincisalia s. str.…”

Section: Charactersmentioning

confidence: 85%

“…In his doctoral thesis Carlos Prieto (2008) revised the whole assemblage on the basis of the biogeography, taxonomy and phylogeny of the group, and recognized many of the genera proposed by Johnson as monophyletic speciesgroups, including the culminicola species group (= Penaincisalia s. s.tr) (Prieto et al 2008(Prieto et al , 2011(Prieto et al and 2016. As the phylogeny of "Penaincisalia" was not fully resolved, the generic concept of Robbins has been used in most species descriptions subsequent to the checklist (Hall et al 2005, Prieto 2007, 2010, but a different view of generic concept has also been expressed , Bálint 2005, Prieto & Bálint 2007, Bálint et al 2008a, 2008b.…”

Section: Introductionmentioning

confidence: 99%

A new species of Penaincisalia(Lepidoptera: Lycaenidae) from Peru

Bálint¹,

Katona²,

Kertész³

2019

Opusc Zool

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Molecular species delimitation in the genus Rhamma Johnson, 1992 (Lepidoptera: Lycaenidae, Theclinae)

Prieto

Núñez

Hausmann

2018

Mitochondrial DNA Part A

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In this work, we used mtDNA data as a tool to delimit species and we compared the resulting molecular operational taxonomic units (barcode index number, BIN) with morphology-based identifications in the Colombian species of Rhamma Johnson, 1992 exploring the usefulness of DNA barcodes for taxonomy, species identification and delimitation. We obtained cytochrome oxidase I (COI) sequences for 134 morphologically identified specimens, representing 12 species of Rhamma from Colombia. Ten of these species have not been previously barcoded. DNA barcodes suggested the potential for eight additional cryptic species in Colombia but we were readily able to morphologically diagnose just one of these linages as a new species which recently was described in a separate paper as Rhamma dawkinsi Prieto & Lorenc-Brudecka, 2017. The morphological species were separated into three categories: species showing a perfect match between morphological species and BINs (33%, four species); species sharing a BIN completely or partly (single specimens) with another morphological species (42%, five species placed in three BINs); and morphological species splitting up into more than one BIN (25%, three species placed in 10 BINs). The high percentages of incongruence between morphology-based identification and species delineation through BINs, could be explained as a consequence of high rates of introgressive hybridization. However, DNA barcodes can be considered diagnostic even in cases where specimens of a species were assigned to two or more distinct BINs and in species showing a low but constant divergence causing their assignment to a single BIN, which is often the case in young, allopatric species. We retain 10 of the 12 species (83%) to be diagnostic in molecular identification.

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ThePenaincisalia amatistaspecies-group (Lepidoptera: Lycaenidae, Eumaeini) in Colombia, insights frommtDNA barcodes and the description of a new species

Cited by 7 publications

References 21 publications

Congruence between morphology-based species and Barcode Index Numbers (BINs) in Neotropical Eumaeini (Lycaenidae)

Congruence between morphology-based species and Barcode Index Numbers (BINs) in Neotropical Eumaeini (Lycaenidae)

A new species of Penaincisalia(Lepidoptera: Lycaenidae) from Peru

Molecular species delimitation in the genus Rhamma Johnson, 1992 (Lepidoptera: Lycaenidae, Theclinae)

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