The PASTICCINO genes of Arabidopsis thaliana are involved in the control of cell division and differentiation

Abstract

The control of cell division by growth regulators is critical to proper plant development. The isolation of single-gene mutants altered in the response to plant hormones should permit the identification of essential genes controlling the growth and development of plants. We have isolated mutants pasticcino belonging to 3 complementation groups (pas1, pas2, pas3) in the progeny of independent ethyl methane sulfonate and T-DNA mutagenized Arabidopsis thaliana plants. The screen was performed in the presence or absence of cytokinin. The mutants isolated were those that showed a significant hypertrophy of their apical parts when grown on cytokinin-containing medium. The pas mutants have altered embryo, leaf and root development. They display uncoordinated cell divisions which are enhanced by cytokinin. Physiological and biochemical analyses show that cytokinins are probably involved in pas phenotypes. The PAS genes have been mapped respectively to chromosomes 3, 5 and 1 and represent new plant genes involved in the control of cell division and plant development.

Sexual and Apogamous Species of Woodferns Show Different Protein and Phytohormone Profiles

“…As noted above, our proteomic analysis revealed a myriad of proteins related to the biosynthesis and/or function of the analyzed phytohormones, giving strong support to the important role of these regulators on gametophyte development. In the apogamous gametophyte, the histidine phosphotransfer protein AHP1, linked to cytokinin signaling was annotated (Hwang et al, 2002 ), and the protein, emb22 (also named Gurke or Pasticcino3), which is an acetyl-CoA carboxylase 1, related to cell proliferation and tissue patterning (Baud et al, 2003 ), embryo morphogenesis (Torres-Ruiz et al, 1996 ); or supressing cytokinin activity (Faure et al, 1998 ). Also the protein Cand1, required for SCF TIR activity, participating in several resposes such as response to auxin (Cheng et al, 2004 ).…”

Integrative Study on Proteomics, Molecular Physiology, and Genetics Reveals an Accumulation of Cyclophilin-Like Protein, TaCYP20-2, Leading to an Increase of Rht Protein and Dwarf in a Novel GA-Insensitive Mutant (gaid) in Wheat

“…According to that, FKBP42 is present in the plasma membrane and interacts with the multidrug resistance/P-glycoprotein (PGP); FKBP42 is proven to play a positive regulatory role in PGP-mediated export of auxin . Studies of the Arabidopsis pas1 mutant indicated that FKBP70 was involved in cell division through cytokinin signaling pathway . Accumulation of the heat-stress protein FKBP77 in wheat also has a negative effect on plant development, especially causing dwarfism .…”

“…47 Studies of the Arabidopsis pas1 mutant indicated that FKBP70 was involved in cell division through cytokinin signaling pathway. 28 Accumulation of the heat-stress protein FKBP77 in wheat also has a negative effect on plant development, especially causing dwarfism. 48 These evidence raise the possibility that the accumulation of TaCYP20-2 may cause dwarfism of gaid through GA signaling pathway.…”

“…27 Mutations in PAS1 (AtFKBP70) leading to tumor growth in plants indicates that FKBP70 is required for correct cell division and cell differentiation. 28 CYP71 regulates plant morphogenesis and serves as a histone remodeling factor involved in chromatin-based silencing. 29 Wheat is an important cereal crop with a complex hexaploid background (approximately 17 000 Mb), which causes difficulties in genomic mutation, gene cloning and genomic modification.…”

“…Lacking a cyclophilin, CYP40, causes a defect in the transition from the juvenile to adult stages of vegetative development and inflorescence morphology in Arabidopsis . Mutations in PAS1 ( AtFKBP70 ) leading to tumor growth in plants indicates that FKBP70 is required for correct cell division and cell differentiation . CYP71 regulates plant morphogenesis and serves as a histone remodeling factor involved in chromatin-based silencing …”