The<i>I</i>Gene of Bean: A Dosage-Dependent Allele Conferring Extreme Resistance, Hypersensitive Resistance, or Spreading Vascular Necrosis in Response to the Potyvirus<i>Bean common mosaic virus</i>

Collmer, Candace Whitmer; Marston, Marcia F.; Taylor, Jane B.; Jahn, Molly M.

doi:10.1094/mpmi.2000.13.11.1266

Cited by 69 publications

(38 citation statements)

References 25 publications

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“…This type of resistance has a considerable degree of phenotypic plasticity. In Phaseolus vulgaris plants that were inoculated with BCMV, manipulation of the I allele gene dosage, the temperature, and the plant cultivar resulted in a continuum of phenotypic responses, including extreme resistance, a resistant hypersensitive reaction, and lethal necrosis (Collmer et al 2000). Several pieces of circumstantial evidence have been reported that suggest both the dominant, hypersensitive-type resistance reaction and lethal necrosis are controlled by the same gene.…”

Section: Discussionmentioning

confidence: 99%

Genetic analysis of lethal tip necrosis induced by Clover yellow vein virus infection in pea

et al. 2007

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Clover yellow vein virus (ClYVV) elicits lethal tip necrosis in the pea line PI 118501. Pea line PI 118501 develops necrotic lesions and veinal necrosis on inoculated leaves, followed by systemic necrosis, leading to plant death. To understand the genetic basis of this lethal tip necrosis, we crossed lines PI 226564 and PI 250438, which develop mosaic symptoms in response to ClYVV inoculation. In reciprocal crosses of PI 118501 with PI 226564, all F 1 plants had mosaic symptoms with slight stem necrosis and early yellowing of upper leaves. Essentially the same symptom was manifested in PI 118501 × PI 250438 F 1 plants. In F 2 populations from the cross between PI 118501 and PI 226564, the observed ratios of necrosis, mosaic with slight stem necrosis, and mosaic fi t the expected 1 : 2 : 1 ratio. These results indicate that a single incompletely dominant gene confers the induction of necrosis in PI 118501. This locus in pea, conferring necrosis induction to ClYVV infection, was designated Cyn1 (Clover yellow vein virus-induced necrosis). A linkage analysis using 100 recombinant inbred lines derived from a cross of PI 118501 and PI 226564 demonstrated that Cyn1 was located 7.5 cM from the SSR marker AD174 on linkage group III.

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Section: Discussionmentioning

confidence: 99%

Genetic analysis of lethal tip necrosis induced by Clover yellow vein virus infection in pea

et al. 2007

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“…A homozygote is immune to BCMV infection, while a heterozygote responds to infection with a HR, which may result in systemic necrosis (Collmer et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Inheritance of resistance to Plum pox virus in apricot (Prunus armeniaca L.)

Karayiannis

Thomidis

Tsaftaris

2007

Tree Genetics & Genomes

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The inheritance of resistance to Plum pox virus (PPV) has been studied in 1,178 apricot hybrids. Seven hundred and eighteen F1 hybrids, obtained from controlled crosses between the susceptible Greek cultivar "Bebecou" and the resistant PPV cultivars of American origin ("Stark Early Orange," 'NJA2," 'Veecot," "Sunglo," "Harlayne," and "Orangered") were evaluated for resistance to the PPV-M (Marcus) strain, 8 years after artificial inoculation. The inheritance of resistance to PPV has been additionally studied for the first time in a BC 1 population of 95 apricot hybrids for four vegetative periods. Reaction of each hybrid to PPV-M was scored through visual symptoms, indexing onto GF-305 and double-antibody sandwich enzyme-linked immunosorbent assay tests. Segregation within the hybrids, determined by Chisquared analysis, fits a 1:1 ratio (P≤0.05) of the resistant vs susceptible, indicating that resistance to PPV is controlled by a single dominant gene locus and that the above six resistant cultivars are heterozygous for the trait. Plants carrying this gene may initially develop disease symptoms on leaves but eventually recover and no virus can be detected in leaves. Susceptible plants show similar symptoms initially but remain symptomatic. Inheritance of resistance to PPV also has been studied in 365 F1 hybrids by crossing the resistant cultivar "Stella" with the susceptible "Bebecou" and the resistant cultivars "Sunglo" and "NJA2," for 8 years after inoculation. The segregation ratio was 1:0 (resistant/susceptible) suggesting that "Stella" is homozygous for the resistance trait. The purpose of this work was the enhancement of the knowledge of inheritance of resistance to PPV for the selection of new cultivars.

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“…In fact, a range of resistance phenotypes can be observed for a single R gene within a single plant species. For example, in the homozygous condition, the dominant I gene in common bean confers extreme resistance (i.e., a symptomless phenotype) to BCMV; whereas in the heterozygous condition, resistance is associated with necrotic lesions (32). In contrast, bean plants homozygous or heterozygous for the I gene develop a systemic necrosis resistance response when infected with the closely related potyvirus, Bean common mosaic necrosis virus.…”

Section: Discussionmentioning

confidence: 99%

A viral resistance gene from common bean functions across plant families and is up-regulated in a non-virus-specific manner

Seo

Rojas

Lee

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

101

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Genes involved in a viral resistance response in common bean (Phaseolus vulgaris cv. Othello) were identified by inoculating a geminivirus reporter (Bean dwarf mosaic virus expressing the green fluorescent protein), extracting RNA from tissue undergoing the defense response, and amplifying sequences with degenerate R gene primers. One such gene (a TIR-NBS-LRR gene, RT4-4) was selected for functional analysis in which transgenic Nicotiana benthamiana were generated and screened for resistance to a range of viruses. This analysis revealed that RT4-4 did not confer resistance to the reporter geminivirus; however, it did activate a resistance-related response (systemic necrosis) to seven strains of Cucumber mosaic virus (CMV) from pepper or tomato, but not to a CMV strain from common bean. Of these eight CMV strains, only the strain from common bean systemically infected common bean cv. Othello. Additional evidence that RT4-4 is a CMV R gene came from the detection of resistance response markers in CMV-challenged leaves of RT4-4 transgenic plants, and the identification of the CMV 2a gene product as the elicitor of the necrosis response. These findings indicate that RT4-4 functions across two plant families and is up-regulated in a non-virus-specific manner. This experimental approach holds promise for providing insights into the mechanisms by which plants activate resistance responses against pathogens.avirulence gene ͉ Cucumber mosaic virus ͉ geminivirus ͉ host defense response ͉ systemic necrosis

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TheIGene of Bean: A Dosage-Dependent Allele Conferring Extreme Resistance, Hypersensitive Resistance, or Spreading Vascular Necrosis in Response to the PotyvirusBean common mosaic virus

Cited by 69 publications

References 25 publications

Genetic analysis of lethal tip necrosis induced by Clover yellow vein virus infection in pea

Genetic analysis of lethal tip necrosis induced by Clover yellow vein virus infection in pea

Inheritance of resistance to Plum pox virus in apricot (Prunus armeniaca L.)

A viral resistance gene from common bean functions across plant families and is up-regulated in a non-virus-specific manner

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