The
            <i>HcrVf2</i>
            gene from a wild apple confers scab resistance to a transgenic cultivated variety

Belfanti, E.; Silfverberg-Dilworth, E.; Tartarini, Stefano; Patocchi, Andrea; Barbieri, Massimo; Zhu, Jun; Vinatzer, Boris A.; Gianfranceschi, L.; Gessler, C.; Sansavini, S.

doi:10.1073/pnas.0304808101

Cited by 222 publications

(140 citation statements)

References 40 publications

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“…In apple, the MdRbcS promoterterminator combination would be suitable to confine the expression of, for example resistance genes, to the leaves which is an important site of infection for microbial diseases and insect pests. Currently, we are testing the suitability of the MdRbcS promoter and terminator for overexpression of the apple HcrVf scab resistance genes (Belfanti et al 2004) in GM apples.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of strong gene regulatory sequences from apple, Malus × domestica

Schaart

Tinnenbroek-Capel

Krens

2010

Tree Genetics & Genomes

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For the strong expression of genes in plant tissue, the availability of specific gene regulatory sequences is desired. We cloned promoter and terminator sequences of an apple (Malus x domestica) ribulose biphosphate carboxylase small subunit gene (MdRbcS), which is known for its high expression and used gus reporter gene expression to test the regulatory activity of the isolated promoter and terminator sequences in transgenic tobacco. The MdRbcS promoter itself seemed to be less strong than the CaMV35S promoter when both used in combination with the nos terminator. However, the combination of the promoter and terminator of MdRbcS was able to drive gus to similar expression levels as the reference construct with CaMV35S promoter and nos terminator. This observation indicates the importance of the terminator sequence for gene expression. It is concluded that the combination of the MdRbcS promoter and terminator is a suitable regulatory sequence set for the expression of transgenes to a high level in plants and for intragenesis in apple specifically.

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Section: Discussionmentioning

confidence: 99%

Isolation and characterization of strong gene regulatory sequences from apple, Malus × domestica

Schaart

Tinnenbroek-Capel

Krens

2010

Tree Genetics & Genomes

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“…A positional cloning project led to the identification in the Vf region of a cluster of genes homologous to the Cf resistance genes of tomato and consequently called HcrVfs (homologues to the Cladosporium fulvum resistance genes of the Vf region, Patocchi et al 1999a, b;Vinatzer et al 2001). One of these genes, HcrVf2, when introduced into the susceptible cultivar Gala, conferred apple scab resistance to the transgenic plants, proving the identification of the first apple scab resistance gene (Barbieri et al 2003;Belfanti et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Molecular markers linked to the apple scab resistance gene Vbj derived from Malus baccata jackii

et al. 2004

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Breeding for scab-resistant apple cultivars by pyramiding several resistance genes in the same genetic background is a promising way to control apple scab caused by the fungus Venturia inaequalis. To achieve this goal, DNA markers linked to the genes of interest are required in order to select seedlings with the desired resistance allele combinations. For several apple scab resistance genes, molecular markers are already available; but until now, none existed for the apple scab resistance gene Vbj originating from the crab apple Malus baccata jackii. Using bulk segregant analysis, three RAPD markers linked to Vbj were first identified. These markers were transformed into more reliable sequence-characterised amplified region (SCAR) markers that proved to be codominant. In addition, three SSR markers and one SCAR were identified by comparing homologous linkage groups of existing genetic maps. Discarding plants showing genotype-phenotype incongruence (GPI plants) plants, a linkage map was calculated. Vbj mapped between the markers CH05e03 (SSR) and T6-SCAR, at 0.6 cM from CH05e03 and at 3.9 cM from T6-SCAR. Without the removal of the GPI plants, Vbj was placed 15 cM away from the closest markers. Problems and pitfalls due to GPI plants and the consequences for mapping the resistance gene accurately are discussed. Finally, the usefulness of co-dominant markers for pedigree analysis is also demonstrated.

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“…Several major genes for resistance to mildew have also been identified and mapped in apple (Alston 1983;Evans and James 2003). To date, only one resistance gene has been isolated and sequenced in apple: the HcrVf2 gene, belonging to the Cf-like class of resistance genes (Belfanti et al 2004). Identification and mapping of RGAs in apple might be a way of discovering new sequences related to disease resistance or finding sequences of previously identified genes.…”

Section: Introductionmentioning

confidence: 99%

Resistance gene analogues identified through the NBS-profiling method map close to major genes and QTL for disease resistance in apple

Calenge

Linden²,

Weg³

et al. 2005

Theor Appl Genet

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We used a new method called nucleotidebinding site (NBS) profiling to identify and map resistance gene analogues (RGAs) in apple. This method simultaneously allows the amplification and the mapping of genetic markers anchored in the conserved NBSencoding domain of plant disease resistance genes. Ninety-four individuals belonging to an F 1 progeny derived from a cross between the apple cultivars 'Discovery' and 'TN10-8' were studied. Two degenerate primers designed from the highly conserved P-loop motif within the NBS domain were used together with adapter primers. Forty-three markers generated with NBS profiling could be mapped in this progeny. After sequencing, 23 markers were identified as RGAs, based on their homologies with known resistance genes or NBS/leucine-rich-repeat-like genes. Markers were mapped on 10 of the 17 linkage groups of the apple genetic map used. Most of these markers were organized in clusters. Twenty-five markers mapped close to major genes or quantitative trait loci for resistance to scab and mildew previously identified in different apple progenies. Several markers could become efficient tools for markerassisted selection once converted into breeder-friendly markers. This study demonstrates the efficiency of the NBS-profiling method for generating RGA markers for resistance loci in apple.

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The HcrVf2 gene from a wild apple confers scab resistance to a transgenic cultivated variety

Cited by 222 publications

References 40 publications

Isolation and characterization of strong gene regulatory sequences from apple, Malus × domestica

Isolation and characterization of strong gene regulatory sequences from apple, Malus × domestica

Molecular markers linked to the apple scab resistance gene Vbj derived from Malus baccata jackii

Resistance gene analogues identified through the NBS-profiling method map close to major genes and QTL for disease resistance in apple

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