The <i>FTF</i> gene family regulates virulence and expression of SIX effectors in <i>Fusarium oxysporum</i>

Niño-Sánchez, Jonathan; Castillo, Virginia Casado-del; Tello, Vega; Vega, Jose J De; Ramos, Brisa; Sukno, Serenella A.; Mínguez, José María

doi:10.1111/mpp.12373

Cited by 47 publications

(89 citation statements)

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“…aTF7- and cTF7- overexpressing strains show a similar growth retardation and reduction of virulence, and aTf1 and cTf1 both are potent inducers of effector gene expression. In addition, a deletion mutant of cTF1 is less virulent, but can still colonize the plant [45,60]. The partial loss of virulence could be a result of redundancy between cTF1 and aTF1 .…”

Section: Discussionmentioning

confidence: 99%

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Transcription Factors Encoded on Core and Accessory Chromosomes of Fusarium oxysporum Induce Expression of Effector Genes

et al. 2016

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Proteins secreted by pathogens during host colonization largely determine the outcome of pathogen-host interactions and are commonly called ‘effectors’. In fungal plant pathogens, coordinated transcriptional up-regulation of effector genes is a key feature of pathogenesis and effectors are often encoded in genomic regions with distinct repeat content, histone code and rate of evolution. In the tomato pathogen Fusarium oxysporum f. sp. lycopersici (Fol), effector genes reside on one of four accessory chromosomes, known as the ‘pathogenicity’ chromosome, which can be exchanged between strains through horizontal transfer. The three other accessory chromosomes in the Fol reference strain may also be important for virulence towards tomato. Expression of effector genes in Fol is highly up-regulated upon infection and requires Sge1, a transcription factor encoded on the core genome. Interestingly, the pathogenicity chromosome itself contains 13 predicted transcription factor genes and for all except one, there is a homolog on the core genome. We determined DNA binding specificity for nine transcription factors using oligonucleotide arrays. The binding sites for homologous transcription factors were highly similar, suggesting that extensive neofunctionalization of DNA binding specificity has not occurred. Several DNA binding sites are enriched on accessory chromosomes, and expression of FTF1, its core homolog FTF2 and SGE1 from a constitutive promoter can induce expression of effector genes. The DNA binding sites of only these three transcription factors are enriched among genes up-regulated during infection. We further show that Ftf1, Ftf2 and Sge1 can activate transcription from their binding sites in yeast. RNAseq analysis revealed that in strains with constitutive expression of FTF1, FTF2 or SGE1, expression of a similar set of plant-responsive genes on the pathogenicity chromosome is induced, including most effector genes. We conclude that the Fol pathogenicity chromosome may be partially transcriptionally autonomous, but there are also extensive transcriptional connections between core and accessory chromosomes.

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Section: Discussionmentioning

confidence: 99%

“…lycopersici and f. sp. phaseoli [60]. Also host-induced gene silencing of aTF1 , potentially silencing the entire gene family, has been reported in F .…”

Section: Discussionmentioning

confidence: 99%

Transcription Factors Encoded on Core and Accessory Chromosomes of Fusarium oxysporum Induce Expression of Effector Genes

et al. 2016

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“…24 , the FTF gene family was further investigated for all the sequenced Fo isolates from onion. A single orthogroup was found to contain all FTF genes previously described in Fo strains, fo47 and Fol-4287 25 as well as the BLAST homologs of FTF genes in Foc and Fo.…”

Section: Foc Carries a Distinct Complement Of Ftf1 Genesmentioning

confidence: 86%

“…Nine transcription factors have been identified on Fol LS regions (TF1-9) 24 . Of these, TF1 ( FTF1 ), a Zn(II)2Cys6-type transcription factor is the best characterised, and regulates SIX gene expression in planta [24][25][26] . However, FTF1 contains a number of homologous genes within the Fol genome, including within core chromosomes 25 .…”

Section: Introductionmentioning

confidence: 99%

“…Of these, TF1 ( FTF1 ), a Zn(II)2Cys6-type transcription factor is the best characterised, and regulates SIX gene expression in planta [24][25][26] . However, FTF1 contains a number of homologous genes within the Fol genome, including within core chromosomes 25 . Transcriptional regulation of LS effector genes is dependent upon factors located on core chromosomes, e.g.…”

Section: Introductionmentioning

confidence: 99%

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