The <i>e</i>-Incubator: A Magnetic Resonance Imaging-Compatible Mini Incubator

Othman, Shadi F.; Wartella, Karin; Sharghi, Vahid Khalilzad; Xu, Huihui

doi:10.1089/ten.tec.2014.0273

Cited by 9 publications

(8 citation statements)

References 26 publications

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“…Komlev et al (2009) applied an advanced X-ray technique, pseudo-holotomography, to simultaneously visualize trabecular mineralized bone and soft tissue including vascular networks with vessels resolved down to 15mm in diameter. The integration of MRI imaging with a sophisticated perfusion bioreactor was demonstrated, showing its potential for non-invasive monitoring of tissue development (Othman et al, 2014); however, there remain concerns regarding the continuous or frequent exposure of cells to powerful magnetic fields (McLeod and Collazo, 2000). Magnetic resonance imaging (MRI) is a useful alternative to mCT, and has the advantage of not exposing the tissue to ionizing radiation (Murphy and Atala, 2014).…”

Section: Experimental Testing and Validationmentioning

confidence: 99%

“…3C). A number of controls are often in place to maintain pH, temperature, dissolved O 2 and CO 2 content (Bartnikowski et al, 2014;Kim and Ma, 2012a;Othman et al, 2014;Zhao et al, 2007). Designs should ensure fluid flow is channeled through the body of the scaffold rather than taking a less restrictive path between the outside of the scaffold and culture chamber walls .…”

Section: Perfusionmentioning

confidence: 99%

“…Perfusion bioreactor designs commonly include on-line monitoring of process conditions such as temperature, flow rate, pH, concentration of dissolved gasses, and nutrients (Dvir et al, 2006;Jaasma et al, 2008;Truscello et al, 2012b;Volkmer et al, 2008). These measurements can be linked with automated controls to maintain levels at a desired rate (Ding et al, 2015;Othman et al, 2014). Additionally, these measurements can be used to validate CFD models and determine changes in scaffold properties over time.…”

Section: Experimental Testing and Validationmentioning

confidence: 99%

“…Magnetic resonance imaging (MRI) is a useful alternative to mCT, and has the advantage of not exposing the tissue to ionizing radiation (Murphy and Atala, 2014). The integration of MRI imaging with a sophisticated perfusion bioreactor was demonstrated, showing its potential for non-invasive monitoring of tissue development (Othman et al, 2014); however, there remain concerns regarding the continuous or frequent exposure of cells to powerful magnetic fields (McLeod and Collazo, 2000).…”

Section: Experimental Testing and Validationmentioning

confidence: 99%

See 3 more Smart Citations

Challenges in engineering large customized bone constructs

Forrestal

Klein

Woodruff

2017

Biotech & Bioengineering

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The ability to treat large tissue defects with customized, patient-specific scaffolds is one of the most exciting applications in the tissue engineering field. While an increasing number of modestly sized tissue engineering solutions are making the transition to clinical use, successfully scaling up to large scaffolds with customized geometry is proving to be a considerable challenge. Managing often conflicting requirements of cell placement, structural integrity, and a hydrodynamic environment supportive of cell culture throughout the entire thickness of the scaffold has driven the continued development of many techniques used in the production, culturing, and characterization of these scaffolds. This review explores a range of technologies and methods relevant to the design and manufacture of large, anatomically accurate tissue-engineered scaffolds with a focus on the interaction of manufactured scaffolds with the dynamic tissue culture fluid environment. Biotechnol. Bioeng. 2017;114: 1129-1139. © 2016 Wiley Periodicals, Inc.

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Section: Experimental Testing and Validationmentioning

confidence: 99%

Section: Perfusionmentioning

confidence: 99%

Section: Experimental Testing and Validationmentioning

confidence: 99%

Section: Experimental Testing and Validationmentioning

confidence: 99%

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Challenges in engineering large customized bone constructs

Forrestal

Klein

Woodruff

2017

Biotech & Bioengineering

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“…Additionally, NMR is ideal for in situ based measurements and dynamic systems where the pore space changes with time. 8,9 However, the maximum diffusion time, D, that can be measured using traditional diffusion-NMR methods, such as the pulsed-gradient spin echo (PGSE) and stimulated echo (PGSTE) experiments, is determined by the relaxation time of the associated spin order that has been prepared; T 2 in PGSE and T 1 in PGSTE. Consequently, there is an upper limit on the pore sizes that can be explored using these traditional methods.…”

Section: Introductionmentioning

confidence: 99%

Singlet-assisted diffusion-NMR (SAD-NMR): redefining the limits when measuring tortuosity in porous media

Tourell

Pop

Brown

et al. 2018

Phys. Chem. Chem. Phys.

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Long-lived singlet order is exploited in diffusion NMR experiments to successfully measure the tortuosity of randomly packed spheres with diameters ranging from 500 to 1000 μm. The pore spaces in such packings have characteristic length scales well beyond the length scale limit set by spin relaxation in conventional NMR-diffusion experiments. Diffusion times of up to 240 s were used to obtain the restricted diffusion coefficient as a function of diffusion time in the long-time diffusion regime. Experimental results were validated with numerical simulations and data from X-ray micro-computed tomography.

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Design and Fabrication of an MRI-Compatible, Autonomous Incubation System

Khalilzad-Sharghi

2015

Ann Biomed Eng

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Tissue engineers have long sought access to an autonomous, imaging-compatible tissue incubation system that, with minimum operator handling, can provide real-time visualization and quantification of cells, tissue constructs, and organs. This type of screening system, capable of operating noninvasively to validate tissue, can overcome current limitations like temperature shock, unsustainable cellular environments, sample contamination, and handling/stress. However, this type of system has been a major challenge, until now. Here, we describe the design, fabrication, and characterization of an innovative, autonomous incubation system that is compatible with a 9.4 T magnetic resonance imaging (MRI) scanner. Termed the e-incubator (patent pending; application number: 13/953,984), this microcontroller-based system is integrated into an MRI scanner and noninvasively screens cells and tissue cultures in an environment where temperature, pH, and media/gas handling are regulated. The 4-week study discussed herein details the continuous operation of the e-incubator for a tissue-engineered osteogenic construct, validated by LIVE/DEAD(®) cell assays and histology. The evolving MR quantitative parameters of the osteogenic construct were used as biomarkers for bone tissue engineering and to further validate the quality of the product noninvasively before harvesting. Importantly, the e-incubator reliably facilitates culturing cells and tissue constructs to create engineered tissues and/or investigate disease therapies.

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The e-Incubator: A Magnetic Resonance Imaging-Compatible Mini Incubator

Cited by 9 publications

References 26 publications

Challenges in engineering large customized bone constructs

Challenges in engineering large customized bone constructs

Singlet-assisted diffusion-NMR (SAD-NMR): redefining the limits when measuring tortuosity in porous media

Design and Fabrication of an MRI-Compatible, Autonomous Incubation System

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