The <i>Drosophila</i> ortholog of vertebrate TRPA1 regulates thermotaxis

Rosenzweig, Mark R.; Brennan, Karen; Tayler, Timothy D.; Phelps, Paul O.; Patapoutian, Ardem; Garrity, Paul

doi:10.1101/gad.1278205

Cited by 271 publications

(294 citation statements)

References 29 publications

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“…Surprisingly, protein expression is not observed in the two regions previously known to contain thermosensitive sensory neurons, multidendritic neurons, and the chordotonal organs [31,33]. In larvae, in which md neurons were silenced by expression of TeTxLC, or in animals lacking chordotonal neurons, no deficits were observed in the thermotactic assay used (thermal gradient from 27 to 41°C) [32]. However, when TeTxLC, or the cell death-promoting gene Hid, is expressed under the putative dTRPA1 promoter, larvae do not fully migrate to the cooler zone (27°C), a partial phenotype of that exhibited after RNAi knockdown of dTRPA1.…”

Section: Trp Ion Channels Mediate Thermosensory Responsesmentioning

confidence: 88%

“…Thus, dTRPA1 likely plays a role in Drosophila thermotaxis but is not required for avoidance and nocifensive responses to noxious heat. Antisera raised against dTRPA1 label primarily a small subset of central brain neurons, neuroendocrine cells of the corpus cardiacum, and unidentified cells adjacent to the mouthhooks and in the digestive system [32]. Surprisingly, protein expression is not observed in the two regions previously known to contain thermosensitive sensory neurons, multidendritic neurons, and the chordotonal organs [31,33].…”

Section: Trp Ion Channels Mediate Thermosensory Responsesmentioning

confidence: 90%

“…Moreover, in a two-temperature choice paradigm, adult flies prefer 22 over 30° C. Similarly, Liu et al [31] showed that Drosophila larvae prefer to reside at 18°C when given the choice of this temperature versus either 11 or 30°C. In another experimental paradigm, larvae placed upon a thermal gradient from 27 to 41°C were shown to thermotax to the coolest temperatures, avoiding those above their optimal growth temperature [32]. What about temperature extremes?…”

Section: Thermotactic and Thermosensory Behaviors In Drosophilamentioning

confidence: 99%

“…Of these, dTRPA1 and dTRPA2, also known as Pyrexia, have been implicated in thermosensation [32,43]. dTRPA1 was first shown to be temperature sensitive in vitro, as threshold temperatures in the warm range (~27°C) activate transient ionic currents when the channel is expressed in heterologous systems [44].…”

Section: Trp Ion Channels Mediate Thermosensory Responsesmentioning

confidence: 99%

“…dTRPA1 was first shown to be temperature sensitive in vitro, as threshold temperatures in the warm range (~27°C) activate transient ionic currents when the channel is expressed in heterologous systems [44]. Moreover, dTRPA1 is essential for larval thermotaxis, as knockdown of channel expression by RNAi prevents these animals from avoiding temperatures above the optimum [32]. This phenotype is specific for dTRPA1 as dsRNAs for the other TRPA family members, as well as the two Drosophila TRPV channels and the lone TRPM, have no affect on this phenotype.…”

Section: Trp Ion Channels Mediate Thermosensory Responsesmentioning

confidence: 99%

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Temperature sensing across species

McKemy

2007

Pflugers Arch - Eur J Physiol

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The ability to detect changes in temperature is a fundamental sensory mechanism for every species and provides organisms with a detailed view of the environment. This review focuses on what is known of the neuronal and molecular substrates for thermosensation across species, focusing on the three robust model systems extensively used to study sensory signaling, the nematode Caenorhabditis elegans, the fruit fly Drosophila melanogaster, and the laboratory mouse. Nematodes migrate to thermal climes that are amenable to their survival, a behavior that is regulated primarily through a single sensory neuron. Additionally, nematodes "learn" to seek out this temperate zone based upon their prior experience, a robust model of learning and memory. Drosophila larvae also prefer select thermal zones that are optimal for growth and have also developed vigorous mechanisms to avoid unfavorable conditions. In mammals, the transduction mechanisms for thermosensation have been identified primarily due to the fact that naturally occurring plant products evoke distinct psychophysical sensation of temperature change. More remarkably, the elucidation of the molecular sensors in mammals, along with those in Drosophila, has demonstrated conservation in the molecular mediators of temperature sensation across diverse species.

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Section: Trp Ion Channels Mediate Thermosensory Responsesmentioning

confidence: 88%

Section: Trp Ion Channels Mediate Thermosensory Responsesmentioning

confidence: 90%

Section: Thermotactic and Thermosensory Behaviors In Drosophilamentioning

confidence: 99%

Section: Trp Ion Channels Mediate Thermosensory Responsesmentioning

confidence: 99%

Section: Trp Ion Channels Mediate Thermosensory Responsesmentioning

confidence: 99%

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Temperature sensing across species

McKemy

2007

Pflugers Arch - Eur J Physiol

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How the TRPA1 receptor transmits painful stimuli: Inner workings revealed by electron cryomicroscopy

Brewster

Gaudet

2015

BioEssays

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Summary A new high-resolution structure of a pain-sensing ion channel, TRPA1, provides a molecular scaffold to understand channel function. Unexpected structural features include a TRP-domain helix similar to TRPV1, a novel ligand-binding site, and an unusual C-terminal coiled coil stabilized by inositol hexakisphosphate (IP6). TRP-domain helices, which structurally act as a nexus for communication between the channel gates and its other domains, may thus be a feature conserved across the entire TRP family and, possibly, other allosterically-gated channels. Similarly, the TRPA1 antagonist-binding site could also represent a druggable location in other ion channels. Combined with known TRPA1 functional properties, the structural role for IP6 leads us to propose that polyphosphate unbinding could act as a molecular kill switch for TRPA1 inactivation. Finally, although packing of the TRPA1 membrane-proximal region hints at a mechanism for electrophile sensing, the details of how TRPA1 responds to noxious reactive electrophiles and temperature await future studies.

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Targeting HSP70 to motoneurons protects locomotor activity from hyperthermia in Drosophila

Xiao

Mileva‐Seitz

Seroude

et al. 2007

Developmental Neurobiology

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Heat shock preconditioning can enhance locomotor and synaptic performance during subsequent hyperthermia. The molecular basis underlying this neural phenotypic modification is largely unknown. Here we report that directing the expression of the 70 kDa heat shock protein (HSP70) to motoneurons protected larval locomotor activity of Drosophila. Tissue-specific expression showed that motoneurons were critical for developing HSP70-mediated thermoprotection of locomotor activity, whereas peripheral sensory neurons, dopaminergic neurons, serotonergic neurons, and muscle cells alone were insufficient. Targeting HSP70 to motoneurons caused structural plasticity of axonal terminals associated with increased transmitter release at neuromuscular junctions at high temperature. The thermoprotection induced by motoneuronal expression of HSP70 mimicked the protective effect of a prior heat shock (36 degrees C, 1 h; 25 degrees C, 1 h) but the effects of heat shock and motoneuronal expression of HSP70 were not additive. In the absence of heat shock pretreatment, ubiquitously expressed transgenic HSP70 activated the transcription of endogenous hsp70 genes. These results demonstrate that motoneurons were critical for HSP70-mediated thermoprotection, and that transgenic HSP70 activated the transcription of endogenous hsp70 in motoneurons with the result that a mix of transgenic and endogenous HSP70 conferred thermoprotection in Drosophila larva.

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The Drosophila ortholog of vertebrate TRPA1 regulates thermotaxis

Cited by 271 publications

References 29 publications

Temperature sensing across species

Temperature sensing across species

How the TRPA1 receptor transmits painful stimuli: Inner workings revealed by electron cryomicroscopy

Targeting HSP70 to motoneurons protects locomotor activity from hyperthermia in Drosophila

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