The <i>Drosophila</i> homolog of methionine sulfoxide reductase A extends lifespan and increases nuclear localization of FOXO

Chung, Hyewon; Kim, Ae-Kyeong; Jung, Sun-Ah; Kim, Si Wouk; Yu, Kweon; Lee, Joon‐Hyung

doi:10.1016/j.febslet.2010.07.033

Cited by 49 publications

(41 citation statements)

References 35 publications

(51 reference statements)

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“…Earlier work identified Eip71CD as a cold-responsive gene in two D. virilis groups [29], with expression levels of Eip71CD being consistently lower during acclimation to low temperature. Given that overexpression of Eip71CD also confers resistance to oxidative stress [30], it is possible that its higher expression levels in lsp2>Sdc-IR flies may be the result of an increase in oxidative stress in these flies. The trade-off, however, is an enhanced sensitivity to cold.…”

Section: Resultsmentioning

confidence: 99%

Knockdown expression of Syndecan in the fat body impacts nutrient metabolism and the organismal response to environmental stresses in Drosophila melanogaster

Eveland

Brokamp

Lue

et al. 2016

Biochemical and Biophysical Research Communications

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The heparan sulfate proteoglycan syndecans are transmembrane proteins involved in multiple physiological processes, including cell-matrix adhesion and inflammation. Recent evidence from model systems and humans suggest that syndecans have a role in energy balance and nutrient metabolism regulation. However, much remains to be learned about the mechanisms through which syndecans influence these phenotypes. Previously, we reported that D. melanogaster Syndecan (Sdc) mutants had reduced metabolic activity compared to controls. Here, we knocked down endogenous Sdc expression in the fat body (the functional equivalent of mammalian adipose tissue and liver) to investigate whether the effects on metabolism originate from this tissue. We found that knocking down Sdc in the fat body leads to flies with higher levels of glycogen and fat and that survive longer during starvation, likely due to their extra energy reserves and an increase in gluconeogenesis. However, compared to control flies, they are also more sensitive to environmental stresses (e.g. bacterial infection and cold) and have reduced metabolic activity under normal feeding conditions. Under the same conditions, fat-body Sdc reduction enhances expression of genes involved in glyceroneogenesis and gluconeogenesis and induces a drastic decrease in phosphorylation levels of AKT and extracellular signal regulated kinase 1/2 (ERK1/2). Altogether, these findings strongly suggest that Drosophila fat body Sdc is involved in a mechanism that shifts resources to different physiological functions according to nutritional status.

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Section: Resultsmentioning

confidence: 99%

Knockdown expression of Syndecan in the fat body impacts nutrient metabolism and the organismal response to environmental stresses in Drosophila melanogaster

Eveland

Brokamp

Lue

et al. 2016

Biochemical and Biophysical Research Communications

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“…The underlying molecular mechanism of MsrA upregulation by AS-IV was explored. A previous study indicated that MsrA expression is regulated via the forkhead box protein/FOXO signaling pathway (30). Sirt1 regulates tyrosine hydroxylase expression and the differentiation of neuroblastoma cells via the FOXO3a signaling pathway (31).…”

Section: As-iv Increases Msra Protein Expression Through the Nad-depementioning

confidence: 99%

Astragaloside IV rescues MPP+-induced mitochondrial dysfunction through upregulation of methionine sulfoxide reductase A

Liu

et al. 2017

Experimental and Therapeutic Medicine

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“…Thus, MsrA overexpression could change the metabolism of methionine that is important for sulfur and DNA methylation pathways involved in aging (42,43). In addition, a forkhead transcription factor, FOXO3a, directly activates the human MSRA gene (34) and its homologs (DAF16 in Caenorhabditis elegans and dFOXO in fruit fly) activate worm msrA (34) and Drosophila msrA genes (39). FOXO3a transcription factor is known to upregulate the expression of genes involved in oxidative stress response and longevity and down-regulate life-shortening genes.…”

Section: Selenium Is An Important Dietary Micronutrient In Mammalsmentioning

confidence: 99%

“…dFOXO overexpression in the adult fatbody of female fruit flies results in increased lifespan (44). Overexpression of Drosophila MsrA caused dFOXO translocation to the nucleus (39), implying that the prolonged lifespan of MsrA-expressing flies may be due not only to the MsrA role in oxidative stress resistance, but also its role in up-regulation of dFOXO. No transcriptional activation of MSRB genes by FOXO3a or its homologs in different organisms was reported thus far.…”

Section: Selenium Is An Important Dietary Micronutrient In Mammalsmentioning

confidence: 99%

Analyses of Fruit Flies That Do Not Express Selenoproteins or Express the Mouse Selenoprotein, Methionine Sulfoxide Reductase B1, Reveal a Role of Selenoproteins in Stress Resistance

Shchedrina

Kabil

Vorbrüggen

et al. 2011

Journal of Biological Chemistry

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Selenoproteins are essential in vertebrates because of their crucial role in cellular redox homeostasis, but some invertebrates that lack selenoproteins have recently been identified. Genetic disruption of selenoprotein biosynthesis had no effect on lifespan and oxidative stress resistance of Drosophila melanogaster. In the current study, fruit flies with knock-out of the selenocysteine-specific elongation factor were metabolically labeled with 75 Se; they did not incorporate selenium into proteins and had the same lifespan on a chemically defined diet with or without selenium supplementation. These flies were, however, more susceptible to starvation than controls, and this effect could be ascribed to the function of selenoprotein K. We further expressed mouse methionine sulfoxide reductase B1 (MsrB1), a selenoenzyme that catalyzes the reduction of oxidized methionine residues and has protein repair function, in the whole body or the nervous system of fruit flies. This exogenous selenoprotein could only be expressed when the Drosophila selenocysteine insertion sequence element was used, whereas the corresponding mouse element did not support selenoprotein synthesis. Ectopic expression of MsrB1 in the nervous system led to an increase in the resistance against oxidative stress and starvation, but did not affect lifespan and reproduction, whereas ubiquitous MsrB1 expression had no effect. Dietary selenium did not influence lifespan of MsrB1-expressing flies. Thus, in contrast to vertebrates, fruit flies preserve only three selenoproteins, which are not essential and play a role only under certain stress conditions, thereby limiting the use of the micronutrient selenium by these organisms. Selenium is an important dietary micronutrient in mammals.The major biological form of selenium is the non-canonical amino acid, selenocysteine (Sec).2 The majority of selenoproteins with known type of catalytic activity act as oxidoreductases that use Sec directly for catalysis and maintenance of cellular redox homeostasis. The number of selenoproteins in eukaryotic organisms varies significantly. Higher plants and fungi lack selenoprotein genes, whereas there are many such genes in algae and vertebrates, e.g. 10 -57 in algae, 30 -37 in fish, and 23-25 in mammals (1). At least five mammalian selenoproteins are essential (2-4). Remarkably, insects possess cysteinecontaining homologs or lack all essential mammalian selenoproteins, e.g. thioredoxin reductases and glutathione peroxidases. Moreover, recent studies identified five species of selenoproteinless insects, including the red flour beetle Tribolium castaneum (5), the silkworm Bombyx mori (5), the fly Drosophila willistoni (6), the honey bee Apis mellifera (6), and the wasp Nasonia vitripennis (6). This evolutionary reduction in the use of selenoproteins could be associated with considerable changes in antioxidant defense systems of insects (7-9).The Sec incorporation machinery is conserved across eukaryotes. Sec is encoded by the UGA codon that usually serves as a terminatio...

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The Drosophila homolog of methionine sulfoxide reductase A extends lifespan and increases nuclear localization of FOXO

Cited by 49 publications

References 35 publications

Knockdown expression of Syndecan in the fat body impacts nutrient metabolism and the organismal response to environmental stresses in Drosophila melanogaster

Knockdown expression of Syndecan in the fat body impacts nutrient metabolism and the organismal response to environmental stresses in Drosophila melanogaster

Astragaloside IV rescues MPP+-induced mitochondrial dysfunction through upregulation of methionine sulfoxide reductase A

Analyses of Fruit Flies That Do Not Express Selenoproteins or Express the Mouse Selenoprotein, Methionine Sulfoxide Reductase B1, Reveal a Role of Selenoproteins in Stress Resistance

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