The <i>dppA</i> gene of <i>Bacillus subtilis</i> encodes a new <scp>d</scp>‐aminopeptidase

Cheggour, Abdelatif; Fanuel, Laurence; Duez, Colette; Joris, Bernard; Bouillenne, Fabrice; Devreese, Bart; Driessche, Gonzalez Van; Beeumen, Jozef Van; Frère, Jean‐Marie; Goffin, Colette

doi:10.1046/j.1365-2958.2000.02117.x

Cited by 35 publications

(30 citation statements)

References 42 publications

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“…These proteins may be involved in recycling of peptidoglycan degradation products . As mentioned earlier in this text, CodY directly regulates Dpp, then the coregulation of the ykfABCD and dpp operons seems to be reasonable since the dpp operon encodes a D-aminopeptidase and a dipeptide uptake system and ykf and dpp operons are functionally related (Mathiopoulos et al, 1991;Cheggour et al, 2000). In our study, YkfB was found to be 3.2-fold down-regulated in the mutant strain, pointing to another gene again under the negative regulation of CodY protein.…”

Section: Other Proteinssupporting

confidence: 79%

“…Nutritional adaptation and spore formation are usually considered as competitive and mutually exclusive processes (Mathiopoulos et al, 1991), but the choice between the two pathways depends on an integration of multiple signals (Cheggour et al, 2000). In our study, DppaA protein was found to be 3.8-fold down-regulated in the mutant strain.…”

Section: Nh 3 -Dependent Nad(+) Synthetase (Sporulation Protein Outb)mentioning

confidence: 51%

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Proteome-wide analysis of the functional roles of bacilysin biosynthesis in Bacillus subtilis

Özcengiz¹,

Taşkin

Demir³

et al. 2014

New Biotechnology

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Section: Other Proteinssupporting

confidence: 79%

Section: Nh 3 -Dependent Nad(+) Synthetase (Sporulation Protein Outb)mentioning

confidence: 51%

Proteome-wide analysis of the functional roles of bacilysin biosynthesis in Bacillus subtilis

Özcengiz¹,

Taşkin

Demir³

et al. 2014

New Biotechnology

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“…These proteins may be involved in recycling of peptidoglycan degradation products. If so, the coregulation of the ykfABCD and dpp operons would be reasonable, since the dpp operon encodes a D-aminopeptidase and a dipeptide uptake system (9,44).…”

Section: Discussionmentioning

confidence: 99%

Additional Targets of theBacillus subtilisGlobal Regulator CodY Identified by Chromatin Immunoprecipitation and Genome-Wide Transcript Analysis

et al. 2003

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Additional targets of CodY, a GTP-activated repressor of early stationary-phase genes in Bacillus subtilis, were identified by combining chromatin immunoprecipitation, DNA microarray hybridization, and gel mobility shift assays. The direct targets of CodY newly identified by this approach included regulatory genes for sporulation, genes that are likely to encode transporters for amino acids and sugars, and the genes for biosynthesis of branched-chain amino acids.Bacteria have evolved a variety of mechanisms to accommodate gene expression to changes in nutritional availability. Some of these mechanisms are specific to a particular gene or operon. In other cases, regulatory proteins control large groups of genes of related function, such as the nitrogen metabolism genes regulated by the Ntr system in enteric bacteria (43) and by TnrA in Bacillus subtilis (17) and the carbon metabolism genes regulated by CcpA in gram-positive bacteria (13) and catabolic gene activator protein-cyclic AMP complex in gram-negative bacteria (59). Even broader forms of regulation are mediated by the leucine-responsive protein (Lrp) of gram-negative bacteria and the sigma-B protein of B. subtilis. Lrp and sigma-B control the transcription of operons that have diverse functions but have a common need to be expressed under a particular set of environmental conditions (50, 54). Lrp regulates the biosynthesis of leucine, isoleucine, valine, serine, glycine, and glutamate; the degradation of serine and threonine; transport of peptides, amino acids, and sugars; and production of fimbriae in response to the availability of leucine and serine (50). Sigma-B activates transcription of a host of genes when cells are exposed to excessive heat, ethanol, salt, or acid (54). Sigma-B responds through a complex, multibranched signal transduction pathway.The B. subtilis CodY protein also has broad effects on gene expression. CodY is a GTP-binding repressor of several genes that are normally quiescent when cells are growing in a rich medium (57). A high concentration of GTP activates CodY as a repressor (57). When the growth rate of B. subtilis slows down because of limitation of the carbon or nitrogen or phosphorus source, the GTP level drops (39, 40), CodY loses repressing activity, and targets of CodY repression are transcribed. The known targets of CodY in B. subtilis include the genes that encode transport systems for dipeptides (dpp) (65) and ␥-aminobutyrate (gabP) (16); catabolic pathways for acetate (acsA) (S. H. Fisher, personal communication), urea (ureABC) (71), histidine (hut) (18), arginine (rocABC and roc-DEF) (B. Belitsky, personal communication), and branchedchain keto acids (the bkd operon) (12); an enzyme of surfactin synthesis (srfAA) (63); the transcription factor for DNA uptake genes (comK) (63); a ComA aspartyl phosphate phosphatase and its inhibitor (rapC-phrC) (37); motility and chemotaxis (hag, fla/che) (45; F. Bergara, C. Ibarra, J. Iwamasa, R. Aguilera, and L. M. Màrquez-Magaña, submitted for publication); and aconitase (citB) (3...

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“…In nitrogen-rich media, L. lactis CodY represses expression of the PrtP proteinase, Opp transporter, and PepN, PepC, and PepO1 peptidases (25). In B. subtilis, the genes encoding peptide transporters Dpp and App, intracellular peptidase DppA, and intracellular protease IspA are known to be negatively regulated by CodY (1,(3)(4)(5)64).…”

Section: Discussionmentioning

confidence: 99%

CodY Regulates Expression of the Bacillus subtilis Extracellular Proteases Vpr and Mpr

et al. 2015

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CodY is a global transcriptional regulator in low-G؉C Gram-positive bacteria that is responsive to GTP and branched-chain amino acids. By interacting with its two cofactors, it is able to sense the nutritional and energetic status of the cell and respond by regulating expression of adaptive genetic programs. In Bacillus subtilis, more than 200 genes, including those for peptide transporters, intracellular proteolytic enzymes, and amino acid degradative pathways, are controlled by CodY. In this study, we demonstrated that expression of two extracellular proteases, Vpr and Mpr, is negatively controlled by CodY. By gel mobility shift and DNase I footprinting assays, we showed that CodY binds to the regulatory regions of both genes, in the vicinity of their transcription start points. The mpr gene is also characterized by the presence of a second, higher-affinity CodY-binding site located at the beginning of its coding sequence. Using strains carrying vpr-or mpr-lacZ transcriptional fusions in which CodY-binding sites were mutated, we demonstrated that repression of both protease genes is due to the direct effect by CodY and that the mpr internal site is required for regulation. The vpr promoter is a rare example of a sigma H-dependent promoter that is regulated by CodY. In a codY null mutant, Vpr became one of the more abundant proteins of the B. subtilis exoproteome. IMPORTANCE CodY is a global transcriptional regulator of metabolism and virulence in low-G؉C

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The dppA gene of Bacillus subtilis encodes a new d‐aminopeptidase

Cited by 35 publications

References 42 publications

Proteome-wide analysis of the functional roles of bacilysin biosynthesis in Bacillus subtilis

Proteome-wide analysis of the functional roles of bacilysin biosynthesis in Bacillus subtilis

Additional Targets of theBacillus subtilisGlobal Regulator CodY Identified by Chromatin Immunoprecipitation and Genome-Wide Transcript Analysis

CodY Regulates Expression of the Bacillus subtilis Extracellular Proteases Vpr and Mpr

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