The<i>Conyza triloba</i>Extracts with High Chlorophyll Content and Free Radical Scavenging Activity Had Anticancer Activity in Cell Lines

El‐Sayed, Wael M.; Hussin, Warda A; Mahmoud, Ahmed A.; Al-Fredan, Mohamed A.

doi:10.1155/2013/945638

Cited by 21 publications

(11 citation statements)

References 30 publications

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“…Beyond this value, the percentage of inhibition appeared to approach a plateau at 32% with corresponding IC 50 of 68 μ g/mL. These observations were consistent with similar findings that high Chl content in crude extracts had antioxidant and protective effects [16]. Therefore, in chlase-treated crude extracts of leaves, unknown components may cooperate with chlide and play a major role in its antioxidant effects.…”

Section: Discussionsupporting

confidence: 86%

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Cytotoxic Effects of Chlorophyllides in Ethanol Crude Extracts from Plant Leaves

Wang

Yang

Huang

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Chlorophyllide (chlide) is a natural catabolic product of chlorophyll (Chl), produced through the activity of chlorophyllase (chlase). The growth inhibitory and antioxidant effects of chlide from different plant leaf extracts have not been reported. The aim of this study is to demonstrate that chlide in crude extracts from leaves has the potential to exert cytotoxic effects on cancer cell lines. The potential inhibitory and antioxidant effects of chlide in crude extracts from 10 plant leaves on breast cancer cells (MCF7 and MDA-MB-231), hepatocellular carcinoma cells (Hep G2), colorectal adenocarcinoma cells (Caco2), and glioblastoma cells (U-118 MG) were studied using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) and DPPH (1,1-diphenyl-2-picrylhydrazyl) assays. The results of the MTT assay showed that chlide in crude extracts from sweet potato were the most effective against all cancer cell lines tested. U-118 MG cells were the most sensitive, while Caco2 cells were the most resistant to the tested crude extracts. The cytotoxic effects of chlide and Chl in crude extracts from sweet potato and of commercial chlorophyllin (Cu-chlin), in descending order, were as follows: chlide > Chl > Cu-chlin. Notably, the IC50 of sweet potato in U-118 MG cells was 45.65 μg/mL while those of Chl and Cu-chlin exceeded 200 μg/mL. In the DPPH assay, low concentrations (100 μg/mL) of chlide and Cu-chlin from crude extracts of sweet potato presented very similar radical scavenging activity to vitamin B2. The concentration of chlide was negatively correlated with DPPH activity. The current study was the first to demonstrate that chlide in crude extracts from leaves have potential cytotoxicity in cancer cell lines. Synergism between chlide and other compounds from leaf crude extracts may contribute to its cytotoxicity.

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Section: Discussionsupporting

confidence: 86%

“…The DPPH assay was used to evaluate the free radical scavenging of chlase-treated crude extract from sweet potato [16]. Briefly, DPPH (8 mg) was dissolved in methanol (100 mL) to obtain a stock solution of 80 μ g/mL.…”

Section: Methodsmentioning

confidence: 99%

Cytotoxic Effects of Chlorophyllides in Ethanol Crude Extracts from Plant Leaves

Wang

Yang

Huang

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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“…Chlorophyll binds to carcinogens in the gastrointestinal tract to dispose them. Chlorophyll resists the growth of cancer cells and acts as an antioxidant in the body …”

Section: Introductionmentioning

confidence: 99%

Sesame Oil Oxidation Control by Active and Smart Packaging System Using Wheat Gluten/Chlorophyll Film to Increase Shelf Life and Detecting Expiration Date

Chavoshizadeh

Pirsa

Mohtarami

2020

Euro J Lipid Sci & Tech

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In this study, a smart biodegradable film based on wheat gluten modified with chlorophyll (WG/Ch) is presented. The effect of chlorophyll on the antioxidant activity and mechanical properties of WG/Ch film is studied. Scanning electron microscopy and Fourier‐transform infrared spectroscopy (FT‐IR) analyses are used to study the structure and chemical composition of gluten‐based films. The WG/Ch film is used in the packaging of sesame oil. The effect of WG/Ch film on increasing the shelf life and detecting expiration time of oil is studied. FT‐IR results show that weak interactions are created between gluten and chlorophyll. The antioxidant activity of the WG film is 60% and it increases to 85% by the addition of chlorophyll. The results show that with the increasing storage time of oil samples, oxidative indices are increased, but the WG/Ch film decreases the sesame oil oxidation significantly (almost in the all oxidative indices 50% decrease is observed). Practical Applications: The WG/Ch film is used in the packaging of sesame oil. The effect of WG/Ch film on the increasing of shelf life and detecting expiration time of oil is studied. By increasing storage time and oxidant, acidity, acid number, PA, and oil color change are increased, but the WG/Ch active film decreases the sesame oil oxidation significantly. Also, by examining the color properties of the active film, it is observed that with increasing storage time and oxidant percent, the color of the film is changed (from green to yellow), which can be used to estimate the oil expiration time. It should be mentioned that the film's color changes are visible to the naked eye.

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“…Green plants possess high chlorophyll contents and show free radical scavenging and anticancer activity. [91] Young plant leaves contain pheophorbide a catabolic product of chlorophyll that shows anticancer activity. [92] Similarly, chlorophyllin shows inhibitory effects against cancer cells and induce chemokine expression.…”

Section: Chlorophyllmentioning

confidence: 99%

Untitled

Upadhyay¹

2018

IJGP

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Present review article explains the dietary use of plant pigments and therapeutic effects against cancer. Important plant pigments such as anthocyanins, lycopene, carotenoids, chlorophyll, and betalains are explained for their anticancer effects. Plant pigments are secondary metabolites which obstruct cancer cell proliferation; stop growth and cell division in cancer cells. These inhibit cellular processes in cancer cells such as signaling pathways, cell cycle, induce apoptosis, and autophagy. Besides, anticancer activity these also assist in controlling high blood pressure, obesity, hyperglycemia, hypercholesterolemia, and restore cardiovascular problems. A full series of pigments is available in various plants families which might show protective effects against cancer. Plant pigments are edible, nutritionally rich and therapeutically suitable. Due to their health-promoting effects there is a growing public interest to consume green vegetables, fruits, sprouted seeds, pigmented cereals, and processed low energy antioxidative functional food. For widening their use, these could be harvested using recombinant gene technology to add to processed foods as a coloring agent. Plant pigments as natural plant products or its by-products are highly useful for the development of a large variety of functional foods, digestive ingredients, additives, as well as cosmetic products. These could be naturally added to genetically suitable modified foods by applying genomic tools. No doubt plant secondary metabolites will also fulfill needs of present-day medicine and show great promise for the future.

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TheConyza trilobaExtracts with High Chlorophyll Content and Free Radical Scavenging Activity Had Anticancer Activity in Cell Lines

Cited by 21 publications

References 30 publications

Cytotoxic Effects of Chlorophyllides in Ethanol Crude Extracts from Plant Leaves

Cytotoxic Effects of Chlorophyllides in Ethanol Crude Extracts from Plant Leaves

Sesame Oil Oxidation Control by Active and Smart Packaging System Using Wheat Gluten/Chlorophyll Film to Increase Shelf Life and Detecting Expiration Date

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