The Arabidopsis thaliana gene AtERF019 negatively regulates plant resistance to Phytophthora parasitica by suppressing PAMP‐triggered immunity

Lu, Wenqin; Deng, Fengyan; Jia, Jinbu; Chen, Xiaokang; Li, Jinfang; Wen, Qujiang; Li, Tingting; Meng, Yuling; Shan, Weixing

doi:10.1111/mpp.12971

Cited by 31 publications

(22 citation statements)

References 70 publications

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“…The objective of TALE‐dependent ERF activation may be the inhibition of responses most useful on Xanthomonas attack through the misactivation of competing defence pathways. Indeed, overexpression of certain ERFs inversely regulated resistance to different pathogens and abiotic stress factors (Broekaert et al., 2006; Li et al., 2018; Lu et al., 2020; Tsutsui et al., 2009).…”

Section: Figurementioning

confidence: 99%

An ERF121 transcription factor from Brassicaoleracea is a target for the conserved TAL‐effectors from different Xanthomonascampestris pv. campestris strains

Zlobin

Lebedeva

Monakhova

et al. 2021

Molecular Plant Pathology

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Section: Figurementioning

confidence: 99%

An ERF121 transcription factor from Brassicaoleracea is a target for the conserved TAL‐effectors from different Xanthomonascampestris pv. campestris strains

Zlobin

Lebedeva

Monakhova

et al. 2021

Molecular Plant Pathology

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“…Phytophthora parasitica is a hemibiotroph pathogen, which means it starts infection as biotroph establishing close contact with living host cells to induce their death later and becomes necrotrophic [18,19]. The A. thaliana ethylene-responsive factor 19 gene (AtERF019) mediates plant susceptibility to P. parasitica through suppression of PTI [28]. P. parasitica have developed strategies to suppress PTI through ER-localised effectors during the biotrophic relationship [21].…”

Section: Discussionmentioning

confidence: 99%

Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae

et al. 2021

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Background The Plutella xylostella PxSDF2L1 gene was previously reported to enhance insect resistance to pathogen at high basal transcription rate. PxSDF2L1 shows similitude with the stromal cell-derived factor 2 (SDF2), an ER stress-induced chaperon protein that is highly conserved throughout animals and plants. The precise biological function of SDF2 is not clear, but its expression is required for innate immunity in plants. Here, we investigate whether a continuous expression of PxSDF2L1 in Nicotiana benthamiana can similarly confer resistance to plant pathogen, particularly, the black shank Phytophthora parasitica var. nicotianae. Results The N. benthamiana plants were inoculated with agrobacteria transformed with a PVX-based binary vector carrying the PxSDF2L1 gene; similar agroinoculation experiments with a PVX vector carrying the GFP gene were used for controls. In pot trials, agroinfected N. benthamiana plants constitutively expressing PxSDF2L1 showed a significant reduction of stem disease symptoms caused by the inoculation with P. parasitica, compared with controls. Conclusions We confirm a role of PxSDF2L1 in resistance to black shank, with a potential application to engineering active resistance against this oomycete in the commercial N. tabacum species and propose its evaluation in other crop families and plant pathogens.

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“…S genes have been classified into those that play a role in host recognition, suppression of host defenses, or in pathogen sustenance and they encode diverse proteins including transporters, protein kinases, membrane-associated proteins (e.g., Mlo), and enzymes (e.g., Dmr6) (Santillan Martinez et al, 2020;Thomazella et al, 2021;van Schie and Takken, 2014;Zheng et al, 2013). Of particular relevance here, several S genes encode transcription factors in the bHLH, bZIP, ERF, and WRKY families (Fan et al, 2014;Fang et al, 2021;Jin et al, 2011;Lu et al, 2020;Prior et al, 2021;Schwartz et al, 2017;Wang et al, 2015a). Similar to Nrd1, a few bHLH transcription factors have been found previously to act as negative regulators of disease resistance in plants.…”

Section: Discussionmentioning

confidence: 99%

Loss of function of bHLH transcription factor Nrd1 in tomato induces an arabinogalactan protein-encoding gene and enhances resistance toPseudomonas syringaepv.tomato

Zhang

Hecht

Sun

et al. 2021

Preprint

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Basic helix-loop-helix (bHLH) transcription factors constitute a superfamily in eukaryotes but their roles in plant immunity remain largely uncharacterized. We found that the transcript abundance in tomato leaves of one bHLH transcription factor-encoding gene, Nrd1 (negative regulator of resistance to DC3000 1), was significantly increased after treatment with the immunity-inducing flgII-28 peptide. Plants carrying a loss-of-function mutation in Nrd1 (Λnrd1) showed enhanced resistance to Pseudomonas syringae pv. tomato (Pst) DC3000 although early pattern-triggered immunity responses such as generation of reactive oxygen species and activation of mitogen-activated protein kinases after treatment with flagellin-derived flg22 and flgII-28 peptides were unaltered compared to wild-type plants. An RNA-Seq analysis identified a gene, Agp1, whose expression is strongly suppressed in an Nrd1-dependent manner. Agp1 encodes an arabinogalactan protein and overexpression of the Agp1 gene in Nicotiana benthamiana led to ∼10-fold less Pst growth compared to the control. These results suggest that the Nrd1 protein promotes tomato susceptibility to Pst by suppressing the defense gene Agp1. RNA-Seq also revealed that loss of Nrd1 function has no effect on the transcript abundance of immunity-associated genes including Bti9, Core, Fls2, Fls3 and Wak1 upon Pst inoculation, suggesting that the enhanced immunity observed in the Δnrd1 mutants is due to the activation of key PRR signaling components as well as loss of Nrd1-regulated suppression of Agp1.

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The Arabidopsis thaliana gene AtERF019 negatively regulates plant resistance to Phytophthora parasitica by suppressing PAMP‐triggered immunity

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 31 publications

References 70 publications

An ERF121 transcription factor from Brassicaoleracea is a target for the conserved TAL‐effectors from different Xanthomonascampestris pv. campestris strains

An ERF121 transcription factor from Brassicaoleracea is a target for the conserved TAL‐effectors from different Xanthomonascampestris pv. campestris strains

Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae

Loss of function of bHLH transcription factor Nrd1 in tomato induces an arabinogalactan protein-encoding gene and enhances resistance toPseudomonas syringaepv.tomato

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