The <i>Arabidopsis</i> homolog of human G3BP1 is a key regulator of stomatal and apoplastic immunity

Abulfaraj, Aala A.; Mariappan, Kadarkaraisamy; Bigeard, Jean; Manickam, Prabhu; Blilou, Ikram; Guo, Xiujie; Al‐Babili, Salim; Pflieger, Delphine; Hirt, Heribert; Rayapuram, Naganand

doi:10.26508/lsa.201800046

Cited by 17 publications

(20 citation statements)

References 59 publications

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“…Seven G3BP-like proteins, within the group of NTF2 and RRM domain family proteins, in A. thaliana were identified and further investigated in this study. Abulfaraj and colleagues 26 described additionally an RNA-binding (RRM-RBD-RNP motif) domain nuclear transport factor 2 family protein (AT3G07250), but in contrast to the other seven, AT3G07250 has a different domain structure, i.e., three RRM domains instead of one and the Gly-rich region and the RG(G) motifs are missing. In addition, the A. thaliana Information Resource (TAIR) database 32 and Klepikova eFP RNAseq data revealed that AT3G07250 is only expressed in flowers 12–14 33 .…”

Section: Discussionmentioning

confidence: 99%

“…For example, the G3BP-like family in A. thaliana has not yet been fully characterized. Abulfaraj and colleagues 26 describe eight family members, generated and analyzed AtG3BP-7 (AT5G48650) OEX lines and KO lines, which showed no phenotype compared to control plants. This might be due to the fact that G3BPs in A. thaliana are redundant in their function, and a KO of one AtG3BP could be compensated by one of the others.…”

Section: Introductionmentioning

confidence: 99%

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Analyzing the G3BP-like gene family of Arabidopsis thaliana in early turnip mosaic virus infection

Reuper

Amari

Krenz

2021

Sci Rep

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The Arabidopsis thaliana genome encodes several genes that are known or predicted to participate in the formation of stress granules (SG). One family of genes encodes for Ras GTPase-activating protein–binding protein (G3BP)-like proteins. Seven genes were identified, of which one of the members was already shown to interact with plant virus proteins in a previous study. A phylogenetic and tissue-specific expression analysis, including laser-dissected phloem, by qRT-PCRs was performed and the sub-cellular localization of individual AtG3BP::EYFP fluorescent fusion proteins expressed in Nicotiana benthamiana epidermal cells was observed. Individual AtG3BP-protein interactions in planta were studied using the bimolecular fluorescence complementation approach in combination with confocal imaging in living cells. In addition, the early and late induction of G3BP-like expression upon Turnip mosaic virus infection was investigated by RNAseq and qRT-PCR. The results showed a high divergence of transcription frequency in the different plant tissues, promiscuous protein–protein interaction within the G3BP-like gene family, and a general induction by a viral infection with TuMV in A. thaliana. The information gained from these studies leads to a better understanding of stress granules, in particular their molecular mode of action in the plant and their role in plant virus infection.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analyzing the G3BP-like gene family of Arabidopsis thaliana in early turnip mosaic virus infection

Reuper

Amari

Krenz

2021

Sci Rep

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“…Similar data is missing for plants, because simultaneous genetic ablation of all seven G3BPs in A. thaliana has not yet been generated. Abulfaraj and colleagues [16] generated and analyzed AtG3BP-7 (AT5G48650) overexpression cell lines and KO lines which showed no phenotype compared to control plants. Our preliminary observations suggest this is also true for the other members of the AtG3BP gene family (data will be presented elsewhere).…”

Section: Discussionmentioning

confidence: 99%

“…It was shown that SG formation requires the NTF2-like and RG/RGG domains [2], and recently it has been demonstrated that these domains are also required for CHIKV replication [14,15]. Sequence analyses revealed seven G3BP-like proteins in Arabidopsis thaliana [16,17], named nuclear transport factor 2 and RNA recognition motif domain-containing proteins (NTF2-RRM proteins). One of the Arabidopsis homologs of human G3BP-1 (named AtG3BP-7 in this study) was shown to be a key regulator of stomatal and apoplastic immunity [16].…”

Section: Introductionmentioning

confidence: 99%

“…Sequence analyses revealed seven G3BP-like proteins in Arabidopsis thaliana [16,17], named nuclear transport factor 2 and RNA recognition motif domain-containing proteins (NTF2-RRM proteins). One of the Arabidopsis homologs of human G3BP-1 (named AtG3BP-7 in this study) was shown to be a key regulator of stomatal and apoplastic immunity [16]. Expression of the A. thaliana G3BP-like proteins (AtG3BPs) in fusion with fluorescence markers showed recruitment of all individual AtG3BPs to cytoplasmic SGs after heat stress, as well as co-localization with ubiquitin carboxyl-terminal hydrolase 24 (AtUBP-24) in bimolecular fluorescence complementation (BiFC) experiments in planta [17,18].…”

Section: Introductionmentioning

confidence: 99%

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Arabidopsis thaliana G3BP Ortholog Rescues Mammalian Stress Granule Phenotype across Kingdoms

Reuper

Götte

Williams

et al. 2021

IJMS

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Stress granules (SGs) are dynamic RNA–protein complexes localized in the cytoplasm that rapidly form under stress conditions and disperse when normal conditions are restored. The formation of SGs depends on the Ras-GAP SH3 domain-binding protein (G3BP). Formations, interactions and functions of plant and human SGs are strikingly similar, suggesting a conserved mechanism. However, functional analyses of plant G3BPs are missing. Thus, members of the Arabidopsis thaliana G3BP (AtG3BP) protein family were investigated in a complementation assay in a human G3BP knock-out cell line. It was shown that two out of seven AtG3BPs were able to complement the function of their human homolog. GFP-AtG3BP fusion proteins co-localized with human SG marker proteins Caprin-1 and eIF4G1 and restored SG formation in G3BP double KO cells. Interaction between AtG3BP-1 and -7 and known human G3BP interaction partners such as Caprin-1 and USP10 was also demonstrated by co-immunoprecipitation. In addition, an RG/RGG domain exchange from Arabidopsis G3BP into the human G3BP background showed the ability for complementation. In summary, our results support a conserved mechanism of SG function over the kingdoms, which will help to further elucidate the biological function of the Arabidopsis G3BP protein family.

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Coronatine Induces Stomatal Reopening by Inhibiting Hormone Signaling Pathways

et al. 2022

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The Arabidopsis homolog of human G3BP1 is a key regulator of stomatal and apoplastic immunity

Abstract: Arabidopsis homolog of human G3BP1 negatively regulates plant immunity and defense responses. Atg3bp1 mutant lines show constitutive stomata closure, expression of a number of key defense marker genes, and accumulate salicylic acid.

Cited by 17 publications

References 59 publications

Analyzing the G3BP-like gene family of Arabidopsis thaliana in early turnip mosaic virus infection

Analyzing the G3BP-like gene family of Arabidopsis thaliana in early turnip mosaic virus infection

Arabidopsis thaliana G3BP Ortholog Rescues Mammalian Stress Granule Phenotype across Kingdoms

Coronatine Induces Stomatal Reopening by Inhibiting Hormone Signaling Pathways

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