The Arabidopsis homeotic genes APETALA3 and PISTILLATA are sufficient to provide the B class organ identity function

Krizek, Beth A.; Meyerowitz, Elliot M.

doi:10.1242/dev.122.1.11

Cited by 380 publications

(28 citation statements)

References 36 publications

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“…We observed previously described phenotypes caused by overexpression of the Arabidopsis PI [35] and PI homologs [29,36], including strong separation between sepals, sepals, and petals narrowing and sepals edge whitening (Figure 5C). The white areas in sepals are attributed to the partial homeotic transformation of sepals into petals [29,35,36]. Our results indicate that AsPI is able to induce B-function identity when ectopically expressed in Arabidopsis plants.…”

Section: Aspi Overexpression In Arabidopsis and Pi-1 Mutant Complementationsupporting

confidence: 63%

“…These organs correspond to filaments (92% of the flowers analyzed/line), filaments ending in stigmatic The ectopic expression of AsPI in a wild-type background modified flower anatomy as expected. We observed previously described phenotypes caused by overexpression of the Arabidopsis PI [35] and PI homologs [29,36], including strong separation between sepals, sepals, and petals narrowing and sepals edge whitening (Figure 5C). The white areas in sepals are attributed to the partial homeotic transformation of sepals into petals [29,35,36].…”

Section: Aspi Overexpression In Arabidopsis and Pi-1 Mutant Complementationsupporting

confidence: 63%

“…The flowers showed partial transformation of sepals into petaloid organs. In addition, sepals are widely separated in 35S::AsPI transgenic flowers, a phenotype associated with the ectopic expression in Arabidopsis of several PI orthologs from distant plant species such as Medicago truncatula [29], Catalpa bungei [35], Lilium longiflorum [51], among others. Interestingly, the ectopic expression of TrPI, a PI ortholog from the eudicot species Taihangia rupestris (Rosaceae) in Arabidopsis caused severe modifications in vegetative plant architecture [53].…”

Section: Discussionmentioning

confidence: 99%

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Isolation and Functional Analysis of a PISTILLATA-like MADS-Box Gene from Argan Tree (Argania spinosa)

Louati

Salazar-Sarasua

Roque

et al. 2021

Plants

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Argan trees (Argania spinosa) belong to a species native to southwestern Morocco, playing an important role in the environment and local economy. Argan oil extracted from kernels has a unique composition and properties. Argan trees were introduced in Tunisia, where hundreds of trees can be found nowadays. In this study, we examined reproductive development in Argan trees from four sites in Tunisia and carried out the functional characterization of a floral homeotic gene in this non-model species. Despite the importance of reproductive development, nothing is known about the genetic network controlling flower development in Argania spinosa. Results obtained in several plant species established that floral organ development is mostly controlled by MADS-box genes and, in particular, APETALA3 (AP3) and PISTILLATA (PI) homologs are required for proper petal and stamen identity. Here, we describe the isolation and functional characterization of a MADS-box gene from Argania spinosa. Phylogenetic analyses showed strong homology with PI-like proteins, and the expression of the gene was found to be restricted to the second and third whorls. Functional homology with Arabidopsis PI was demonstrated by the ability of AsPI to confer petal and stamen identity when overexpressed in a pi-1 mutant background. The identification and characterization of this gene support the strong conservation of PI homologs among distant angiosperm plants.

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Section: Aspi Overexpression In Arabidopsis and Pi-1 Mutant Complementationsupporting

confidence: 63%

Section: Aspi Overexpression In Arabidopsis and Pi-1 Mutant Complementationsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation and Functional Analysis of a PISTILLATA-like MADS-Box Gene from Argan Tree (Argania spinosa)

Louati

Salazar-Sarasua

Roque

et al. 2021

Plants

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“…AP3 and PI are necessary to provide petal and stamen identity in the Arabidopsis flower. Also, AP3 and PI are sufficient to bestow petal and stamen identity in conjunction with class A and C genes, respectively (Krizek and Meyerowitz, 1996; Wuest et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

De novo Transcriptome Assembly and Dynamic Spatial Gene Expression Analysis in Red Clover

Chakrabarti¹,

Dinkins

Hunt³

2016

The Plant Genome

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Red clover (Trifolium pratense L.) is a cool-season forage legume grown throughout the northeastern United States and is the most widely planted forage legume after alfalfa (Medicago sativa L.). Red clover provides high-value feed to the livestock because of high protein content and easy digestibility. To date, genomic resources for red clover are scarce. In the current study, a de novo transcriptome assembly of red clover was constructed representing different tissue types. The draft assembly consists of 37,565 contigs with N50 and average contig length of 1707 and 1262 bp, respectively. A comparative study with three other legume species displayed a high degree of sequence conservation between red clover and other legumes. The assembled transcriptome was annotated to allow identification of desirable genes. In particular, a genome-wide identification of red clover transcripts encoding putative transcription factors was performed. A comparative gene expression analysis between different tissue types was performed using the assembled transcriptome as the reference, which revealed dynamic gene expression patterns across different tissue types and also identified spatially dynamic gene coexpression clusters. Genes representing tissue-enriched clusters were subjected to gene ontology (GO) enrichment analysis to identify over-represented functional groups. Identification of these tissue-enriched gene coexpression clusters can help in future research focusing on developmental studies across tissues or in biotechnological improvement of red clover. Red clover is a diploid (2n = 14), naturally crosspollinated, cool-season forage legume (Smith et al., 1985). In general, clover has three primary centers of diversity: Eurasia, America, and Africa. Red clover probably originated in southeastern Europe and Asia Minor and inhabits temperate regions of the world . In the United States, it is the most widely grown forage legume after alfalfa and is grown throughout the northeastern United States. If the climate is favorable, red clover can adapt to a wide range of soils. It can fix atmospheric N through symbiosis with Rhizobium species and is estimated to fix approximately 125 to 220 kg Abbreviations: CEG, core eukaryotic gene; CEGMA, core eukaryotic genes mapping approach; EC, Enzyme Commission; EST, expressed sequence tag; GO, gene ontology; KAAS, KEGG Automatic Annotation Server; KEGG, Kyoto Encyclopedia of Genes and Genomes; KO, KEGG Orthology; LHC, light-harvesting complex; PlnTFDB, Plant Transcription Factor Database; PS, photosystem; RNA-seq, RNA sequencing. Core Ideas• De novo transcriptome assembly of red clover (Trifolium pratense L.)• Functional annotation of the assembled transcriptome• Global identification of red clover transcripts encoding putative transcription factors• Identification of tissue-enriched gene coexpression clusters

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“…The estradiol-inducible LEC2 lines (indLEC2) were generated by amplifying the LEC2 coding sequence by PCR and cloning in pBI-ΔGR vector. The LEC2-GR fragment was then cloned in pCGN18 51 to create p35S::LEC2-GR. Plants were transformed by floral dipping, transgenic plants were selected in Kanamycin (indLEC2) or RFP seed selection (indRKD4), and propagated for three generations to isolate homozygous lines.…”

Section: Plasmid Constructs and Transgenic Linesmentioning

confidence: 99%

Predictable and stable epimutations induced during clonal propagation with embryonic transcription factors

Wibowo

Antunez-Sanchez

Dawson

et al. 2022

Preprint

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Although clonal propagation is frequently used in commercial plant breeding and plant biotechnology programs because it minimizes genetic variation, it is not uncommon to observe clonal plants with stable phenotypic changes, a phenomenon known as somaclonal variation. Several studies have shown that epigenetic modifications induced during regeneration are associated with this newly acquired phenotypic variation. However, the factors that determine the extent of somaclonal variation and the molecular changes associated with it remain poorly understood. To address this gap in our knowledge, we compared clonally propagated Arabidopsis thaliana plants derived from somatic embryogenesis using two different embryonic transcription factors- RWP-RK DOMAIN-CONTAINING 4 (RKD4) or LEAFY COTYLEDON2 (LEC2) and from two epigenetically distinct tissues. We found that both the epi(genetic) status of explant and the regeneration protocol employed play critical roles in shaping the molecular and phenotypic state of clonal plants. Phenotypic variation of regenerated plants can be largely explained by the inheritance of tissue-specific DNA methylation imprints, which are associated with specific transcriptional and metabolic changes in sexual progeny of clonal plants. Moreover, regenerants from roots were particularly affected by the inheritance of epigenetic imprints, which resulted in increased accumulation of salicylic acid in leaves and accelerated plant senescence. Collectively, our data reveal pathways for targeted manipulation of phenotypic variation in clonal plants.

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The Arabidopsis homeotic genes APETALA3 and PISTILLATA are sufficient to provide the B class organ identity function

Cited by 380 publications

References 36 publications

Isolation and Functional Analysis of a PISTILLATA-like MADS-Box Gene from Argan Tree (Argania spinosa)

Isolation and Functional Analysis of a PISTILLATA-like MADS-Box Gene from Argan Tree (Argania spinosa)

De novo Transcriptome Assembly and Dynamic Spatial Gene Expression Analysis in Red Clover

Predictable and stable epimutations induced during clonal propagation with embryonic transcription factors

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