The<i>Arabidopsis</i>Floral Homeotic Proteins APETALA3 and PISTILLATA Negatively Regulate the<i>BANQUO</i>Genes Implicated in Light Signaling

Mara, Chloe; Huang, Tengbo; Irish, Vivian F.

doi:10.1105/tpc.109.065946

Cited by 73 publications

(75 citation statements)

References 83 publications

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“…For example, PRE1/BNQ1, PRE2/BNQ2, and PRE4/BNQ3 are direct target genes negatively regulated by AP3/PI in the petals and therefore play a role in floral development (Mara et al, 2010). The pre4/bnq3 mutant has decreased chlorophyll levels causing pale-white sepals and carpels (Mara et al, 2010). PRE3/ ATBS1/TMO7 was identified as target gene of MONOPTEROS/ ARF5 that is required for embryonic root development.…”

Section: Discussionmentioning

confidence: 99%

“…Many studies have demonstrated that the PRE family of HLH factors plays a key role in promoting cell elongation and plant growth downstream of many environmental and hormonal signals Hyun and Lee, 2006;Zhang et al, 2009;Wang et al, 2009;Mara et al, 2010;Schlereth et al, 2010). Several PRE family members have been shown to interact with and inhibit atypical bHLH factors, including IBH1 and AIFs (Hyun and Lee, 2006;Zhang et al, 2009;Wang et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…The Arabidopsis PRE family includes six members (PRE1/BNQ1, PRE2/BNQ3, PRE3/ ATBS1/TMO7, PRE4/BNQ3, PRE5, and PRE6/KDR), which display distinct but also overlapping functions in plant growth and development (Hyun and Lee, 2006;Lee et al, 2006;Wang et al, 2009;Zhang et al, 2009;Mara et al, 2010;Schlereth et al, 2010). For example, PRE1/BNQ1, PRE2/BNQ2, and PRE4/BNQ3 are direct target genes negatively regulated by AP3/PI in the petals and therefore play a role in floral development (Mara et al, 2010). The pre4/bnq3 mutant has decreased chlorophyll levels causing pale-white sepals and carpels (Mara et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

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A Triple Helix-Loop-Helix/Basic Helix-Loop-Helix Cascade Controls Cell Elongation Downstream of Multiple Hormonal and Environmental Signaling Pathways inArabidopsis

et al. 2012

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Environmental and endogenous signals, including light, temperature, brassinosteroid (BR), and gibberellin (GA), regulate cell elongation largely by influencing the expression of the paclobutrazol-resistant (PRE) family helix-loop-helix (HLH) factors, which promote cell elongation by interacting antagonistically with another HLH factor, IBH1. However, the molecular mechanism by which PREs and IBH1 regulate gene expression has remained unknown. Here, we show that IBH1 interacts with and inhibits a DNA binding basic helix-loop-helix (bHLH) protein, HBI1, in Arabidopsis thaliana. Overexpression of HBI1 increased hypocotyl and petiole elongation, whereas dominant inactivation of HBI1 and its homologs caused a dwarf phenotype, indicating that HBI1 is a positive regulator of cell elongation. In vitro and in vivo experiments showed that HBI1 directly bound to the promoters and activated two EXPANSIN genes encoding cell wall-loosening enzymes; HBI1's DNA binding and transcriptional activities were inhibited by IBH1, but the inhibitory effects of IBH1 were abolished by PRE1. The results indicate that PREs activate the DNA binding bHLH factor HBI1 by sequestering its inhibitor IBH1. Altering each of the three factors affected plant sensitivities to BR, GA, temperature, and light. Our study demonstrates that PREs, IBH1, and HBI1 form a chain of antagonistic switches that regulates cell elongation downstream of multiple external and endogenous signals.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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A Triple Helix-Loop-Helix/Basic Helix-Loop-Helix Cascade Controls Cell Elongation Downstream of Multiple Hormonal and Environmental Signaling Pathways inArabidopsis

et al. 2012

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“…AP3 and PI form heterodimers and appears to act together with other MADS box proteins viz. AP1 and SEPALLATA (SEP) to regulate development of floral organs (Mara et al 2010;Yi and Jack 1998). AP3-PI heterodimer bind to the promoter of AP3 and drives its expression through an auto-regulatory positive feedback loop (Mara et al 2010;Yi and Jack 1998).…”

Section: Introductionmentioning

confidence: 99%

“…AP1 and SEPALLATA (SEP) to regulate development of floral organs (Mara et al 2010;Yi and Jack 1998). AP3-PI heterodimer bind to the promoter of AP3 and drives its expression through an auto-regulatory positive feedback loop (Mara et al 2010;Yi and Jack 1998). Two transcripts of CsAP3 gene have been reported in saffron, namely, CsAP3a & CsAP3b, which differ in CCT trinucleotide repeats in 5′ and 3′ UTRs (Tsaftaris et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Comparative expression analysis of senescence gene CsNAP and B-class floral development gene CsAP3 during different stages of flower development in Saffron (Crocus sativus L.)

Wafai

Bukhari

Mokhdomi

et al. 2015

Physiol Mol Biol Plants

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Crocus sativus, a monocot triploid species belonging to the Iridaceae family, is cultivated for its red stigmatic lobes of the carpel that constitute saffron. Flower development has been extensively studied in different plants. Different floral developmental pathways have been deciphered in many plants. In Crocus sativus, flower is the most important part and understanding the pathway underlying the flower development can pave the way for new avenues to improve its productivity and quality. The combination of class A genes (including APETALA1; CsAP1 and APETALA2; CsAP2), class B genes (including APETALA3; CsAP3 and PISTILLATA; CsPI) and class C genes (including AGAMOUS; CsAG) that are active in each whorl, determines the identity of the organs that will later develop in that whorl. CsAP3 is a class B homeotic gene which promotes petal and stamen formation and has a very important role in flower development. It also activates other genes playing pivotal role in flower development. It has been earlier reported that CsAP3 gene has direct role in activation of CsNAP gene which promotes senescence in plants. Present work was focused on study of relative gene expression changes of CsAP3 and CsNAP gene during different stages of flower development.CsAP3 gene expression was found maximum during latepreanthesis stages of stigma development. Expression increases from stage 5 to stage 6 of flower development and then reduces again from stage 6 to stage 7. CsNAP gene had moderate expression during stage 3 to stage 4 transition and its expression increased abruptly from stage 6 to stage 7 of flower development. There is no direct concordance in the expression of CsAP3 and CsNAP gene expression in saffron. We may conclude that some other factor(s) may be responsible for initiation of CsNAP expression and CsAP3 gene may directly/indirectly be involved in regulating the factors responsible for CsNAP activation.

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Phenotypic, genetic and molecular characterization of 7B-1, a conditional male-sterile mutant in tomato

2017

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We characterized the photoperiod-sensitive 7B - 1 male-sterile mutant in tomato, showing its allelism with stamenless - 2 . Mapping experiments indicated SlGLO2 , a B-class MADS-box family member, as a strong candidate to underlie the 7B - 1 mutation. The interest in male sterility (MS) dates back to a long time due to its perspective use in hybrid seed production. Here, we characterize 7B-1, a photoperiod-sensitive male-sterile (ms) mutant in tomato (Solanum lycopersicum L.), in which stamens are restored to fertility by permissive growth conditions in short days (SD). This system represents a useful strategy to facilitate the maintenance of the ms line. Examination of 7B-1 and other structural mutants, vms, sl, sl-2 and tap3, showed carpellization of stamens in the third floral whorl. 7B-1 exhibits strong expressivity in long days (LD), producing 100% aberrant anthers and virtually no seed production under open pollination, whereas it recovered fertility in SD. By genetic analysis, we demonstrate that 7B-1 is not allelic to sl nor to vms; instead it shows allelism to sl-2. Because the homeotic phenotype of the mutation resembles lesions to members of the B-class MADS-box transcription factor family, that specify petal and stamen identity, we pursued a candidate gene approach towards these targets. Using an interspecific backcross mapping population and markers linked to B-class MADS-box genes, significant linkage was found between 7B-1 and the SlGLO2 gene on Chr6. This result was supported by the 7B-1 phenotype that is similar to that of SlGLO2 knock outs and by the strong downregulation of the gene in the mutant. Although the lesion underlying the mutant phenotype is still elusive, our results pave the way for the final demonstration that SlGLO2 underlies 7B-1 and further the use of 7B-1 mutant in tomato hybrid seed production schemes.

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TheArabidopsisFloral Homeotic Proteins APETALA3 and PISTILLATA Negatively Regulate theBANQUOGenes Implicated in Light Signaling

Cited by 73 publications

References 83 publications

A Triple Helix-Loop-Helix/Basic Helix-Loop-Helix Cascade Controls Cell Elongation Downstream of Multiple Hormonal and Environmental Signaling Pathways inArabidopsis

A Triple Helix-Loop-Helix/Basic Helix-Loop-Helix Cascade Controls Cell Elongation Downstream of Multiple Hormonal and Environmental Signaling Pathways inArabidopsis

Comparative expression analysis of senescence gene CsNAP and B-class floral development gene CsAP3 during different stages of flower development in Saffron (Crocus sativus L.)

Phenotypic, genetic and molecular characterization of 7B-1, a conditional male-sterile mutant in tomato

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