The <i>Arabidopsis ABORTED MICROSPORES</i> (<i>AMS</i>) gene encodes a MYC class transcription factor

Sørensen, Anna; Kröber, Sandra; Unte, Ulrike S.; Huijser, Peter; Dekker, Koen; Saedler, Heinz

doi:10.1046/j.1365-313x.2003.01644.x

Cited by 378 publications

(365 citation statements)

References 49 publications

Supporting

Mentioning

349

Contrasting

Unclassified

Order By: Relevance

“…Identification of putative orthologs in different species will benefit the study of gene function, such as AtMYC2 (Abe et al, 2003) and OsMYC (Zhu et al, 2005), AMS (AtbHLH021; Sorensen et al, 2003) Zhang, unpublished data). The identity and similarity of their full-length sequences were about 32% and 42%, respectively, and 70% and 76% within the two bHLH domains.…”

Section: The Expression Pattern Of Osbhlh and Atbhlh Genesmentioning

confidence: 99%

“…The identity and similarity of their full-length sequences were about 32% and 42%, respectively, and 70% and 76% within the two bHLH domains. AMS is critical for tapetal cell differentiation and likely regulates a postmeiotic transcriptional program supporting microspore development (Sorensen et al, 2003). Similar to ams, loss of function of TDR seems to result in delayed tapetal cell degradation and causes complete male sterility.…”

Section: The Expression Pattern Of Osbhlh and Atbhlh Genesmentioning

confidence: 99%

See 1 more Smart Citation

Genome-Wide Analysis of Basic/Helix-Loop-Helix Transcription Factor Family in Rice and Arabidopsis

et al. 2006

View full text Add to dashboard Cite

The basic/helix-loop-helix (bHLH) transcription factors and their homologs form a large family in plant and animal genomes. They are known to play important roles in the specification of tissue types in animals. On the other hand, few plant bHLH proteins have been studied functionally. Recent completion of whole genome sequences of model plants Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa) allows genome-wide analysis and comparison of the bHLH family in flowering plants. We have identified 167 bHLH genes in the rice genome, and their phylogenetic analysis indicates that they form well-supported clades, which are defined as subfamilies. In addition, sequence analysis of potential DNA-binding activity, the sequence motifs outside the bHLH domain, and the conservation of intron/exon structural patterns further support the evolutionary relationships among these proteins. The genome distribution of rice bHLH genes strongly supports the hypothesis that genome-wide and tandem duplication contributed to the expansion of the bHLH gene family, consistent with the birth-and-death theory of gene family evolution. Bioinformatics analysis suggests that rice bHLH proteins can potentially participate in a variety of combinatorial interactions, endowing them with the capacity to regulate a multitude of transcriptional programs. In addition, similar expression patterns suggest functional conservation between some rice bHLH genes and their close Arabidopsis homologs.

show abstract

Section: The Expression Pattern Of Osbhlh and Atbhlh Genesmentioning

confidence: 99%

Section: The Expression Pattern Of Osbhlh and Atbhlh Genesmentioning

confidence: 99%

Genome-Wide Analysis of Basic/Helix-Loop-Helix Transcription Factor Family in Rice and Arabidopsis

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Expression patterns similar to that of At1g27710 have been described for several other regulators of microsporogenesis that are essential for normal pollen formation. These genes included ABORTED MICROSPORES (At2g16910; Sorensen et al, 2003), MALE MEIOCYTE DEATH1 (At1g66170), MYB80 (At5g56110; Li et al, 1999;Stracke et al, 2001), and MS2 (At3g11980; Aarts et al, 1997). As for At2g42940 and At1g27710, we found that most of these genes also showed a prolonged expression in ms1 mutant flowers (Fig.…”

Section: Validation Of Predicted Expression Patternsmentioning

confidence: 99%

“…Genetic analyses have led to the isolation of several mutants with distinct defects in stamen development (Dawson et al, 1993;Sanders et al, 1999;Schiefthaler et al, 1999;Yang et al, 1999;Canales et al, 2002;Zhao et al, 2002;Reddy et al, 2003;Sorensen et al, 2003;Steiner-Lange et al, 2003;Albrecht et al, 2005). Cloning of the genes affected in these mutants resulted in the identification of key regulators of stamen formation.…”

mentioning

confidence: 99%

Global Expression Profiling Applied to the Analysis of Arabidopsis Stamen Development

et al. 2007

View full text Add to dashboard Cite

To obtain detailed information about gene expression during stamen development in Arabidopsis (Arabidopsis thaliana), we compared, by microarray analysis, the gene expression profile of wild-type inflorescences to those of the floral mutants apetala3, sporocyteless/nozzle, and male sterile1 (ms1), in which different aspects of stamen formation are disrupted. These experiments led to the identification of groups of genes with predicted expression at early, intermediate, and late stages of stamen development. Validation experiments using in situ hybridization confirmed the predicted expression patterns. Additional experiments aimed at characterizing gene expression specifically during microspore formation. To this end, we compared the gene expression profiles of wild-type flowers of distinct developmental stages to those of the ms1 mutant. Computational analysis of the datasets derived from this experiment led to the identification of genes that are likely involved in the control of key developmental processes during microsporogenesis. We also identified a large number of genes whose expression is prolonged in ms1 mutant flowers compared to the wild type. This result suggests that MS1, which encodes a putative transcriptional regulator, is involved in the stage-specific repression of these genes. Lastly, we applied reverse genetics to characterize several of the genes identified in the microarray experiments and uncovered novel regulators of microsporogenesis, including the transcription factor MYB99 and a putative phosphatidylinositol 4-kinase.

show abstract

“…Tapetal cell development and differentiation are critical for the early events in male reproduction, including meiosis; however, during late pollen development, tapetal degeneration, triggered by an apoptosis-like process, is also vital for viable pollen formation (Papini et al, 1999;Varnier et al, 2005;Li et al, 2006;Aya et al, 2009). Currently, although several genes encoding putative transcription factors have been reported to be associated with tapetal function and degeneration, such as Arabidopsis (Arabidopsis thaliana) MYB33/MYB65 (Millar and Gubler, 2005), DYSFUNCTIONAL TAPETUM1 (DYT1; Zhang et al, 2006), ABORTED MICROSPORE (AMS; Sorensen et al, 2003;Xu et al, 2010), and MALE STERILITY1 (MS1; Wilson et al, 2001;Ito and Shinozaki, 2002) and rice (Oryza sativa) GAMYB (Kaneko et al, 2004;Aya et al, 2009;Liu et al, 2010), UNDEVELOPED TAPETUM1 (UDT1; Jung et al, 2005), TAPETUM DE-GENERATION RETARDATION (TDR; Li et al, 2006), and MADS3 (Hu et al, 2011), their detailed functional roles in regulating tapetal PCD during anther development are unclear. We have shown that the Arabidopsis ms1 mutant displays altered tapetal development, with a lack of normal PCD and abnormal tapetal degeneration associated with large autophagic vacuoles and mitochondrial swelling (Vizcay-Barrena and Wilson, 2006).…”

mentioning

confidence: 99%

PERSISTENT TAPETAL CELL1Encodes a PHD-Finger Protein That Is Required for Tapetal Cell Death and Pollen Development in Rice

et al. 2011

View full text Add to dashboard Cite

In higher plants, timely degradation of tapetal cells, the innermost sporophytic cells of the anther wall layer, is a prerequisite for the development of viable pollen grains. However, relatively little is known about the mechanism underlying programmed tapetal cell development and degradation. Here, we report a key regulator in monocot rice (Oryza sativa), PERSISTANT TAPETAL CELL1 (PTC1), which controls programmed tapetal development and functional pollen formation. The evolutionary significance of PTC1 was revealed by partial genetic complementation of the homologous mutation MALE STERILITY1 (MS1) in the dicot Arabidopsis (Arabidopsis thaliana). PTC1 encodes a PHD-finger (for plant homeodomain) protein, which is expressed specifically in tapetal cells and microspores during anther development in stages 8 and 9, when the wild-type tapetal cells initiate a typical apoptosis-like cell death. Even though ptc1 mutants show phenotypic similarity to ms1 in a lack of tapetal DNA fragmentation, delayed tapetal degeneration, as well as abnormal pollen wall formation and aborted microspore development, the ptc1 mutant displays a previously unreported phenotype of uncontrolled tapetal proliferation and subsequent commencement of necrosis-like tapetal death. Microarray analysis indicated that 2,417 tapetum-and microspore-expressed genes, which are principally associated with tapetal development, degeneration, and pollen wall formation, had changed expression in ptc1 anthers. Moreover, the regulatory role of PTC1 in anther development was revealed by comparison with MS1 and other rice anther developmental regulators. These findings suggest a diversified and conserved switch of PTC1/MS1 in regulating programmed male reproductive development in both dicots and monocots, which provides new insights in plant anther development.

show abstract

The Arabidopsis ABORTED MICROSPORES (AMS) gene encodes a MYC class transcription factor

Cited by 378 publications

References 49 publications

Genome-Wide Analysis of Basic/Helix-Loop-Helix Transcription Factor Family in Rice and Arabidopsis

Genome-Wide Analysis of Basic/Helix-Loop-Helix Transcription Factor Family in Rice and Arabidopsis

Global Expression Profiling Applied to the Analysis of Arabidopsis Stamen Development

PERSISTENT TAPETAL CELL1Encodes a PHD-Finger Protein That Is Required for Tapetal Cell Death and Pollen Development in Rice

Contact Info

Product

Resources

About