The <i>Aquilegia pubiflora</i> (Himalayan columbine) mediated synthesis of nanoceria for diverse biomedical applications

Jan, Hasnain; Khan, Muhammad Aslam; Usman, Hazrat; Shah, Muzamil; Ansir, Rotaba; Faisal, Shah; Ullah, Niamat; Rahman, Lubna

doi:10.1039/d0ra01971b

Cited by 63 publications

(25 citation statements)

References 58 publications

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“…Orientin and chlorogenic acid protect the plant from stress conditions and perform various biological activities such as antioxidant, antiaging, anti-inflammatory, antidiabetic, antifungal, antibacterial, hepatoprotective, and anticancer (Lam et al, 2016;Naveed et al, 2018). The presence of flavonoids and hydroxycinnamic acid derivatives on the surface of nanoparticles is according to the results of our previous reports (Jan et al, 2020;Shah et al, 2020). These plant active compounds are considered to be involved in the capping of ZnO-NPs, as shown in Figure 2.…”

Section: Biocompatibility Of Zno Nanoparticles With Rbcsmentioning

confidence: 74%

Biogenic Synthesis and Characterization of Antimicrobial and Antiparasitic Zinc Oxide (ZnO) Nanoparticles Using Aqueous Extracts of the Himalayan Columbine (Aquilegia pubiflora)

et al. 2020

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Herein, we report a facile, economic, one-pot green synthesis of zinc oxide nanoparticles (ZnO-NPs) for diverse biomedical applications. In the study, ZnO-NPs were synthesized using an aqueous extract of Aquilegia pubiflora as an effective reducing and capping agent. The biomediated nanoparticles were characterized using various techniques including high-performance liquid chromatography (HPLC), X-ray diffraction, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy, Dynamic light scattering, photoluminescence, and RAMAN. The particles were highly pure, having an average size of 34.23 nm with spherical or elliptical morphology, and displayed good aqueous dispersion capability. FTIR and HPLC confirmed the successful capping of flavonoids and hydroxycinnamic acid derivatives. The characterized NPs were then explored for their antimicrobial and anti-leishmanial potential. Among the tested bacterial and fungal strains, ZnO-NPs were more potent toward Pseudomonas aeruginosa and Fusarium solani with inhibition zone of 10.3 ± 0.19 mm and 13 ± 1.4 mm, respectively. A dose-dependent cytotoxic effect was observed against Leishmania tropica (KWH23) with significant IC 50 for both the promastigote (48 µg/mL) and amastigote form (51 µg/mL) of the parasite. In addition, bacterial kinase enzymes were inhibited by ZnO-NPs, thus allowing us to elaborate a possible action mechanism. Finally, the remarkable biocompatible nature of the particles was confirmed against freshly isolated human red blood cells (hRBCs). Altogether, these results affirmed the high antimicrobial and antiparasitic potential of ZnO-NPs obtained through a biogenic synthetic approach using aqueous extract of the Himalayan Columbine (Aquilegia pubiflora).

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Section: Biocompatibility Of Zno Nanoparticles With Rbcsmentioning

confidence: 74%

Biogenic Synthesis and Characterization of Antimicrobial and Antiparasitic Zinc Oxide (ZnO) Nanoparticles Using Aqueous Extracts of the Himalayan Columbine (Aquilegia pubiflora)

et al. 2020

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“…Himalayan Columbine (Aquilegia pubiflora Wall. Ex Royle) is a medicinal plant that has been used for decades to cure skin burns, jaundice, hepatitis, wound healing, cardiovascular and circulation problems [111].…”

Section: Discussionmentioning

confidence: 99%

“…The purpose of this study was to investigate the phytochemical constituents and further evaluate the antimicrobial, anti-parasitic, anti-Alzheimer, and cytotoxic properties of the Aquilegia pubiflora extracts (AP DW , AP M , APE, and AP EA ). Genus Aquilegia belongs to the Ranunculaceae family, which has more than 60 species of plants that are used primarily in South Asia and [67,111]. Antibiotic resistance is a severe problem that affects healthcare systems in both developing and developed countries across the world.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical analysis and versatile in vitro evaluation of antimicrobial, cytotoxic and enzyme inhibition potential of different extracts of traditionally used Aquilegia pubiflora Wall. Ex Royle

Jan

Usman

Shah

et al. 2021

BMC Complement Med Ther

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Background Himalayan Columbine (Aquilegia pubiflora Wall. Ex Royle) is a medicinal plant and have been used as traditional treatments for various human diseases including skin burns, jaundice, hepatitis, wound healing, cardiovascular and circulatory diseases. Till now there is no report available on phytochemical investigation of Himalayan Columbine and to the best of our knowledge, through present study we have reported for the first time, the phytochemical analysis and pharmacological potentials of different leaf extracts of Aquilegia pubiflora. Methods Four types of extracts were prepared using solvent of different polarities (Distilled water APDW, Methanol APM, Ethanol APE and Ethyl acetate APEA), and were evaluated to determine the best candidate for potent bioactivity. Phytochemical constituents in prepared extracts were quantified through HPLC analysis. Subsequently, all four types of leaf extracts were then evaluated for their potential bioactivities including antimicrobial, protein kinase inhibition, anti-inflammatory, anti-diabetic, antioxidant, anti-Alzheimer, anti-aging and cytotoxic effect. Results HPLC analysis demonstrated the presence of dvitexin, isovitexin, orientin, isoorientin, ferulic acid, sinapic acid and chlorogenic acid in varied proportions in all plant extracts. Antimicrobial studies showed that, K. pneumonia was found to be most susceptible to inhibition zones of 11.2 ± 0.47, 13.9 ± 0.33, 12.7 ± 0.41, and 13.5 ± 0.62 measured at 5 mg/mL for APDW, APM, APE and APEA respectively. A. niger was the most susceptible strain in case of APDW with the highest zone of inhibition 14.3 ± 0.32, 13.2 ± 0.41 in case of APM, 13.7 ± 0.39 for APE while 15.4 ± 0.43 zone of inhibition was recorded in case of APEA at 5 mg/mL. The highest antioxidant activity of 92.6 ± 1.8 μgAAE/mg, 89.2 ± 2.4 μgAAE/mg, 277.5 ± 2.9 μM, 289.9 ± 1.74 μM for TAC, TRP, ABTS and FRAP, respectively, was shown by APE. APM, APE and APEA extracts showed a significant % cell inhibition (above 40%) against HepG2 cells. The highest anti-inflammatory of the samples was shown by APE (52.5 ± 1.1) against sPLA2, (41.2 ± 0.8) against 15-LOX, followed by (38.5 ± 1.5) and (32.4 ± 0.8) against COX-1 and COX-2, respectively. Conclusions Strong antimicrobial, Protein Kinase potency and considerable α-glucosidase, α-amylase, and cytotoxic potential were exhibited by plant samples. Significant anti-Alzheimer, anti-inflammatory, anti-aging, and kinase inhibitory potential of each plant sample thus aware us for further detailed research to determine novel drugs.

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“…The anti-leishmanial potential of the plant extracts was assessed against the amastigote and promastigote cultures of L. tropica KWH23 (Department of Biotechnology IIUI Pakistan) ( Ahmad et al, 2015 , Jan et al, 2020a , Jan et al, 2020b ). M199 media having 10% fetal bovine serum helped for culturing leishmanial parasites.…”

Section: Methodsmentioning

confidence: 99%

In vitro examination of anti-parasitic, anti-Alzheimer, insecticidal and cytotoxic potential of Ajuga bracteosa Wallich leaves extracts

Imran

Jan

Faisal

et al. 2021

Saudi Journal of Biological Sciences

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This research study is mainly focused to evaluate the anti-parasitic, insecticidal, cytotoxic and anti-alzheimer potential of various leaf extracts of Ajuga bracteosa Wallich ex Bentham. 04 different extracts were prepared using solvent of different polarity to determine the best candidate for potent bioactivity i.e. n-hexane (NH), Ethyl acetate (EA), Ethanol (EL) and Chloroform (CH). Concentrations of each extracts were made specified for all activities. All extracts were exploited for broad range of biomedical applications including leishmaniasis, in vitro anti-Alzheimer, insecticidal and cytotoxic studies. Our results showed that A. bracteosa n-hexane extract was highly active against Leishmania Tropica with significant inhibition of 58 ± 1.61 for promastigote and 63 ± 2.29 for amastigote at 1000 μg/mL. Furthermore, promising anti-alzheimer activity acetylcholinesterase (AChE) 46 ± 0.83 and butrylcholineterase (BChE) 49 ± 1.17 was noted for n-hexane. The insecticidal potential of these extracts were test against five different insects ( Rhyzopertha dominica, Trogoderma granarium, Tribolium castaneum, Sitophilus oryze, and Callosobruchus analis ). The higest mortality rate of insecticidal activity was recorded by n-hexane followed by Ethyl acetate whereas ethanol extract was found to be less effective against all the test species. Significant cytotoxic potential of each plant sample against Artemia salina thus aware us for further detailed research to find out novel drugs. Based on our results we believe that Ajuga bracteosa could be used to develop as a potential botanical insecticide against different insect and pests, such as aphids as well as an excellent source for the compound isolation as anti-tumor agent.

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The Aquilegia pubiflora (Himalayan columbine) mediated synthesis of nanoceria for diverse biomedical applications

Abstract: Graphical illustration of eco-friendly, facile, one-pot, green synthesis of nanoceria for multiple biomedical applications.

Cited by 63 publications

References 58 publications

Biogenic Synthesis and Characterization of Antimicrobial and Antiparasitic Zinc Oxide (ZnO) Nanoparticles Using Aqueous Extracts of the Himalayan Columbine (Aquilegia pubiflora)

Biogenic Synthesis and Characterization of Antimicrobial and Antiparasitic Zinc Oxide (ZnO) Nanoparticles Using Aqueous Extracts of the Himalayan Columbine (Aquilegia pubiflora)

Phytochemical analysis and versatile in vitro evaluation of antimicrobial, cytotoxic and enzyme inhibition potential of different extracts of traditionally used Aquilegia pubiflora Wall. Ex Royle

In vitro examination of anti-parasitic, anti-Alzheimer, insecticidal and cytotoxic potential of Ajuga bracteosa Wallich leaves extracts

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